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Article: Detection and serogroup differerntiation of Salmonella spp. in food within 30 hours by enrichment-immunoassay with a T6 monoclonal antibody capture enzyme-linked immunosorbent assay

TitleDetection and serogroup differerntiation of Salmonella spp. in food within 30 hours by enrichment-immunoassay with a T6 monoclonal antibody capture enzyme-linked immunosorbent assay
Authors
Issue Date1996
PublisherAmerican Society for Microbiology.
Citation
Applied and Environmental Microbiology, 1996, v. 62 n. 7, p. 2294-2302 How to Cite?
AbstractWe previously described an antigen capture enzyme-linked immunosorbent assay which makes use of monoclonal antibody T6, which recognizes an epitope on the outer core polysaccharide of Salmonella lipopolysaccharide molecules that is common to almost all Salmonella serovars. In this paper, we show that this assay can detect between 10(5) and 10(7) Salmonella cells per ml even in the presence of excess Escherichia coli. A total of 153 of 154 (99%) serogroup A to E strains and 51 of 78 (71%) serogroup F to 67 strains were reactive as determined by this assay. This corresponds to a detection rate of approximately 98% of all salmonellae known to affect humans. None of the 65 strains of non-Salmonella bacteria tested positive. Taking advantage of the O-factor polysaccharides also present on the antigen captured by the immobilized T6 antibody, we showed that 136 of 154 Salmonella serogroup A to E strains (88%) were correctly differentiated according to their serogroups by use of enzyme conjugates of a panel of O-factor-specific monoclonal antibodies. We evaluated this assay for the detection and serogroup differentiation of salmonellae directly from enrichment cultures of simulated food, eggs, pork, and infant formula milk. All 26 samples which had been contaminated with Salmonella spp. were detected by T6 (100% sensitivity), with only one false-positive result from 101 samples not contaminated by Salmonella spp. (99% specificity). The detection time was substantially reduced to between 17 and 29 h, depending on the enrichment methods used. Since there were no false-negative results, we concluded that this enrichment-immunoassay method can afford rapid screening for Salmonella spp. in food samples.
Persistent Identifierhttp://hdl.handle.net/10722/49178
ISSN
2015 Impact Factor: 3.823
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PubMed Central ID
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DC FieldValueLanguage
dc.contributor.authorNg, SPen_HK
dc.contributor.authorTsui, COen_HK
dc.contributor.authorRoberts, Den_HK
dc.contributor.authorChau, PYen_HK
dc.contributor.authorNg, MHen_HK
dc.date.accessioned2008-06-12T06:36:09Z-
dc.date.available2008-06-12T06:36:09Z-
dc.date.issued1996en_HK
dc.identifier.citationApplied and Environmental Microbiology, 1996, v. 62 n. 7, p. 2294-2302en_HK
dc.identifier.issn0099-2240en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49178-
dc.description.abstractWe previously described an antigen capture enzyme-linked immunosorbent assay which makes use of monoclonal antibody T6, which recognizes an epitope on the outer core polysaccharide of Salmonella lipopolysaccharide molecules that is common to almost all Salmonella serovars. In this paper, we show that this assay can detect between 10(5) and 10(7) Salmonella cells per ml even in the presence of excess Escherichia coli. A total of 153 of 154 (99%) serogroup A to E strains and 51 of 78 (71%) serogroup F to 67 strains were reactive as determined by this assay. This corresponds to a detection rate of approximately 98% of all salmonellae known to affect humans. None of the 65 strains of non-Salmonella bacteria tested positive. Taking advantage of the O-factor polysaccharides also present on the antigen captured by the immobilized T6 antibody, we showed that 136 of 154 Salmonella serogroup A to E strains (88%) were correctly differentiated according to their serogroups by use of enzyme conjugates of a panel of O-factor-specific monoclonal antibodies. We evaluated this assay for the detection and serogroup differentiation of salmonellae directly from enrichment cultures of simulated food, eggs, pork, and infant formula milk. All 26 samples which had been contaminated with Salmonella spp. were detected by T6 (100% sensitivity), with only one false-positive result from 101 samples not contaminated by Salmonella spp. (99% specificity). The detection time was substantially reduced to between 17 and 29 h, depending on the enrichment methods used. Since there were no false-negative results, we concluded that this enrichment-immunoassay method can afford rapid screening for Salmonella spp. in food samples.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsApplied and Environmental Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Applied and Environmental Microbiology, 1996, v. 62 n. 7, p. 2294-2302en_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methods - statistics & numerical dataen_HK
dc.subject.meshFood Microbiologyen_HK
dc.subject.meshSalmonella - classification - immunology - isolation & purificationen_HK
dc.subject.meshEscherichia coli - isolation & purificationen_HK
dc.subject.meshEvaluation Studies as Topicen_HK
dc.titleDetection and serogroup differerntiation of Salmonella spp. in food within 30 hours by enrichment-immunoassay with a T6 monoclonal antibody capture enzyme-linked immunosorbent assayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0099-2240&volume=62&issue=7&spage=2294&epage=2302&date=1996&atitle=Detection+and+serogroup+differerntiation+of+Salmonella+spp.+in+food+within+30+hours+by+enrichment-immunoassay+with+a+T6+monoclonal+antibody+capture+enzyme-linked+immunosorbent+assayen_HK
dc.identifier.emailNg, SP: spng@hku.hken_HK
dc.identifier.emailNg, MH: hrmmnmh@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8779567en_HK
dc.identifier.pmcidPMC168010-
dc.identifier.hkuros14555-
dc.identifier.isiWOS:A1996UV79200014-

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