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Article: Human parainfluenza virus 4 outbreak and the role of diagnostic tests

TitleHuman parainfluenza virus 4 outbreak and the role of diagnostic tests
Authors
Issue Date2005
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2005, v. 43 n. 9, p. 4515-4521 How to Cite?
Abstract
Owing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, involving 38 institutionalized children and three staff members, during a 3-week period in autumn 2004. Most subjects had upper respiratory tract infections (URTI), while lower respiratory tract infections (LRTI) occurred in three children (7%), one complicated by respiratory failure requiring ventilation support. All patients recovered. Nasopharyngeal aspirates tested for HPIV-4 were positive by reverse transcriptase PCR (RT-PCR) in all 41 cases (100%), by direct immunofluorescence in 29 of 39 tested cases (74%), and by cell cultures in 6 of 37 cases (16%), and serum was positive for antibodies against HPIV-4 in all 35 cases (100%) with serum samples available. In addition, RT-PCR detected HPIV-4 in four children (three LRTI and one URTI) out of 115 patients with community-acquired respiratory tract infection. Molecular analysis of the 1,198-bp phosphoprotein sequences showed that HPIV-4 isolates among the cases were genetically similar, whereas the community controls were more genetically distant, supporting nosocomial transmission of a single HPIV-4 genotype during the outbreak. Moreover, the HPIV-4 causing the outbreak is more closely related to HPIV-4A than HPIV-4B. HPIV-4 may be an important cause of more severe respiratory illness in children. The present RT-PCR assay is a sensitive, specific, and rapid method for the diagnosing HPIV-4 infection. To better define the epidemiology and clinical spectrum of disease of HPIV-4 infections, HPIV-4 should be included in the routine panels of respiratory virus detection on respiratory specimens. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/49171
ISSN
2013 Impact Factor: 4.232
PubMed Central ID
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Centre for Health Protection
  3. Caritas Medical Centre Hong Kong
  4. Chinese University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorTo, WKen_HK
dc.contributor.authorTse, PWTen_HK
dc.contributor.authorChan, AKHen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorLeung, AFYen_HK
dc.contributor.authorLi, KSMen_HK
dc.contributor.authorChan, PKSen_HK
dc.contributor.authorLim, WWLen_HK
dc.contributor.authorYung, RWHen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:00Z-
dc.date.available2008-06-12T06:36:00Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2005, v. 43 n. 9, p. 4515-4521en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49171-
dc.description.abstractOwing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, involving 38 institutionalized children and three staff members, during a 3-week period in autumn 2004. Most subjects had upper respiratory tract infections (URTI), while lower respiratory tract infections (LRTI) occurred in three children (7%), one complicated by respiratory failure requiring ventilation support. All patients recovered. Nasopharyngeal aspirates tested for HPIV-4 were positive by reverse transcriptase PCR (RT-PCR) in all 41 cases (100%), by direct immunofluorescence in 29 of 39 tested cases (74%), and by cell cultures in 6 of 37 cases (16%), and serum was positive for antibodies against HPIV-4 in all 35 cases (100%) with serum samples available. In addition, RT-PCR detected HPIV-4 in four children (three LRTI and one URTI) out of 115 patients with community-acquired respiratory tract infection. Molecular analysis of the 1,198-bp phosphoprotein sequences showed that HPIV-4 isolates among the cases were genetically similar, whereas the community controls were more genetically distant, supporting nosocomial transmission of a single HPIV-4 genotype during the outbreak. Moreover, the HPIV-4 causing the outbreak is more closely related to HPIV-4A than HPIV-4B. HPIV-4 may be an important cause of more severe respiratory illness in children. The present RT-PCR assay is a sensitive, specific, and rapid method for the diagnosing HPIV-4 infection. To better define the epidemiology and clinical spectrum of disease of HPIV-4 infections, HPIV-4 should be included in the routine panels of respiratory virus detection on respiratory specimens. Copyright © 2005, American Society for Microbiology. All Rights Reserved.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2005, v. 43 n. 9, p. 4515-4521en_HK
dc.subject.meshCross Infection - diagnosis - epidemiology - virologyen_HK
dc.subject.meshDisease Outbreaksen_HK
dc.subject.meshParainfluenza Virus 4, Human - classification - genetics - isolation & purificationen_HK
dc.subject.meshRubulavirus Infections - diagnosis - epidemiology - virologyen_HK
dc.subject.meshAntibodies, Viral - blooden_HK
dc.titleHuman parainfluenza virus 4 outbreak and the role of diagnostic testsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=43&issue=9&spage=4515&epage=4521&date=2005&atitle=Human+parainfluenza+virus+4+outbreak+and+the+role+of+diagnostic+testsen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.43.9.4515-4521.2005en_HK
dc.identifier.pmid16145100en_HK
dc.identifier.pmcidPMC1234116en_HK
dc.identifier.scopuseid_2-s2.0-24744440562en_HK
dc.identifier.hkuros114674-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24744440562&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue9en_HK
dc.identifier.spage4515en_HK
dc.identifier.epage4521en_HK
dc.identifier.isiWOS:000232020400031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridTo, WK=7004294514en_HK
dc.identifier.scopusauthoridTse, PWT=7005336881en_HK
dc.identifier.scopusauthoridChan, AKH=8669885400en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridLeung, AFY=8907665900en_HK
dc.identifier.scopusauthoridLi, KSM=24759122500en_HK
dc.identifier.scopusauthoridChan, PKS=7403497792en_HK
dc.identifier.scopusauthoridLim, WWL=7202378267en_HK
dc.identifier.scopusauthoridYung, RWH=7005594277en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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