Article: Molecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms

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TitleMolecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms
AuthorsKan, B3
Wang, M1
Jing, H3
Xu, H1
Jiang, X3
Yan, M3
Liang, W3
Zheng, H3
Wan, K3
Liu, Q3
Cui, B3
Xu, Y3
Zhang, E3
Wang, H3
Ye, J3
Li, G3
Li, M3
Cui, Z3
Qi, X3
Chen, K1
Du, L1
Gao, K1
Zhao, YT1
Zou, XZ1
Feng, YJ1
Gao, YF1
Hai, R3
Yu, D3
Guan, Y2
Xu, J3
Issue Date2005
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
CitationJournal Of Virology, 2005, v. 79 n. 18, p. 11892-11900 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.18.11892-11900.2005
AbstractMassive numbers of palm civets were culled to remove sources for the reemergence of severe acute respiratory syndrome (SARS) in Guangdong Province, China, in January 2004, following SARS coronavirus detection in market animals. The virus was identified in all 91 palm civets and 15 raccoon dogs of animal market origin sampled prior to culling, but not in 1,107 palm civets later sampled at 25 farms, spread over 12 provinces, which were claimed to be the source of traded animals. Twenty-seven novel signature variation residues (SNVs) were identified on the spike gene and were analyzed for their phylogenetic relationships, based on 17 sequences obtained from animals in our study and from other published studies. Analysis indicated that the virus in palm civets at the live-animal market had evolved to infect humans. The evolutionary starting point was a prototype group consisting of three viral sequences of animal origin. Initially, seven SNV sites caused six amino acid changes, at positions 147, 228, 240, 479, 821, and 1080 of the spike protein, to generate low-pathogenicity viruses. One of these was linked to the first SARS patient in the 2003-2004 period. A further 14 SNVs caused 11 amino acid residue changes, at positions 360, 462, 472, 480, 487, 609, 613, 665, 743, 765, and 1163. The resulting high-pathogenicity groups were responsible for infections during the so-called early-phase epidemic of 2003. Finally, the remaining six SNVs caused four amino acid changes, at positions 227, 244, 344, and 778, which resulted in the group of viruses responsible for the global epidemic. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
DescriptionErratum in: Journal of Virology 2006 Aug;80(15):7786
ISSN0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
DOIhttp://dx.doi.org/10.1128/JVI.79.18.11892-11900.2005
PubMed Central IDPMC1212604
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorKan, B
dc.contributor.authorWang, M
dc.contributor.authorJing, H
dc.contributor.authorXu, H
dc.contributor.authorJiang, X
dc.contributor.authorYan, M
dc.contributor.authorLiang, W
dc.contributor.authorZheng, H
dc.contributor.authorWan, K
dc.contributor.authorLiu, Q
dc.contributor.authorCui, B
dc.contributor.authorXu, Y
dc.contributor.authorZhang, E
dc.contributor.authorWang, H
dc.contributor.authorYe, J
dc.contributor.authorLi, G
dc.contributor.authorLi, M
dc.contributor.authorCui, Z
dc.contributor.authorQi, X
dc.contributor.authorChen, K
dc.contributor.authorDu, L
dc.contributor.authorGao, K
dc.contributor.authorZhao, YT
dc.contributor.authorZou, XZ
dc.contributor.authorFeng, YJ
dc.contributor.authorGao, YF
dc.contributor.authorHai, R
dc.contributor.authorYu, D
dc.contributor.authorGuan, Y
dc.contributor.authorXu, J
dc.date.accessioned2008-06-12T06:35:54Z
dc.date.available2008-06-12T06:35:54Z
dc.date.issued2005
dc.description.abstractMassive numbers of palm civets were culled to remove sources for the reemergence of severe acute respiratory syndrome (SARS) in Guangdong Province, China, in January 2004, following SARS coronavirus detection in market animals. The virus was identified in all 91 palm civets and 15 raccoon dogs of animal market origin sampled prior to culling, but not in 1,107 palm civets later sampled at 25 farms, spread over 12 provinces, which were claimed to be the source of traded animals. Twenty-seven novel signature variation residues (SNVs) were identified on the spike gene and were analyzed for their phylogenetic relationships, based on 17 sequences obtained from animals in our study and from other published studies. Analysis indicated that the virus in palm civets at the live-animal market had evolved to infect humans. The evolutionary starting point was a prototype group consisting of three viral sequences of animal origin. Initially, seven SNV sites caused six amino acid changes, at positions 147, 228, 240, 479, 821, and 1080 of the spike protein, to generate low-pathogenicity viruses. One of these was linked to the first SARS patient in the 2003-2004 period. A further 14 SNVs caused 11 amino acid residue changes, at positions 360, 462, 472, 480, 487, 609, 613, 665, 743, 765, and 1163. The resulting high-pathogenicity groups were responsible for infections during the so-called early-phase epidemic of 2003. Finally, the remaining six SNVs caused four amino acid changes, at positions 227, 244, 344, and 778, which resulted in the group of viruses responsible for the global epidemic. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
dc.description.naturepublished_or_final_version
dc.descriptionErratum in: Journal of Virology 2006 Aug;80(15):7786
dc.format.extent388 bytes
dc.format.mimetypetext/html
dc.identifier.citationJournal Of Virology, 2005, v. 79 n. 18, p. 11892-11900 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.18.11892-11900.2005
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.79.18.11892-11900.2005
dc.identifier.epage11900
dc.identifier.hkuros110152
dc.identifier.isiWOS:000231633900034
dc.identifier.issn0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
dc.identifier.issue18
dc.identifier.openurl
dc.identifier.pmcidPMC1212604
dc.identifier.pmid16140765
dc.identifier.scopuseid_2-s2.0-24644457565
dc.identifier.spage11892
dc.identifier.urihttp://hdl.handle.net/10722/49166
dc.identifier.volume79
dc.languageeng
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Virology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2005, v. 79 n. 18, p. 11892-11900
dc.subject.meshEvolution, Molecular
dc.subject.meshSARS Virus - classification - genetics - isolation & purification
dc.subject.meshViverridae - virology
dc.subject.meshSevere Acute Respiratory Syndrome - epidemiology - transmission - virology
dc.subject.meshViral Envelope Proteins - genetics
dc.titleMolecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms
dc.typeArticle
Author Affiliations
  1. Guangdong Center for Disease Control and Prevention
  2. The University of Hong Kong
  3. Chinese Center for Disease Control and Prevention