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Article: Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2

TitleReceptor and viral determinants of SARS-coronavirus adaptation to human ACE2
Authors
KeywordsAngiotensin-converting enzyme 2
Coronavirus
Severe acute respiratory syndrome
Viral adaptation
Issue Date2005
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.html
Citation
Embo Journal, 2005, v. 24 n. 8, p. 1634-1643 How to Cite?
AbstractHuman angiotensin-converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS-CoV). Here we identify the SARS-CoV spike (S)-protein-binding site on ACE2. We also compare S proteins of SARS-CoV isolated during the 2002-2003 SARS outbreak and during the much less severe 2003-2004 outbreak, and from palm civets, a possible source of SARS-CoV found in humans. All three S proteins bound to and utilized palm-civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S-protein-binding site of human ACE2 to those of civet ACE2, or by altering S-protein residues 479 and 487 to residues conserved during the 2002-2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS-CoV to human cells, and provide insight into the severity of the 2002-2003 SARS epidemic. © 2005 European Molecular Biology Organization | All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/49159
ISSN
2015 Impact Factor: 9.643
2015 SCImago Journal Rankings: 7.450
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Wen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorSui, Jen_HK
dc.contributor.authorKuhn, JHen_HK
dc.contributor.authorMoore, MJen_HK
dc.contributor.authorLuo, Sen_HK
dc.contributor.authorWong, SKen_HK
dc.contributor.authorHuang, ICen_HK
dc.contributor.authorXu, Ken_HK
dc.contributor.authorVasilieva, Nen_HK
dc.contributor.authorMurakami, Aen_HK
dc.contributor.authorHe, Yen_HK
dc.contributor.authorMarasco, WAen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorChoe, Hen_HK
dc.contributor.authorFarzan, Men_HK
dc.date.accessioned2008-06-12T06:35:45Z-
dc.date.available2008-06-12T06:35:45Z-
dc.date.issued2005en_HK
dc.identifier.citationEmbo Journal, 2005, v. 24 n. 8, p. 1634-1643en_HK
dc.identifier.issn0261-4189en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49159-
dc.description.abstractHuman angiotensin-converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS-CoV). Here we identify the SARS-CoV spike (S)-protein-binding site on ACE2. We also compare S proteins of SARS-CoV isolated during the 2002-2003 SARS outbreak and during the much less severe 2003-2004 outbreak, and from palm civets, a possible source of SARS-CoV found in humans. All three S proteins bound to and utilized palm-civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S-protein-binding site of human ACE2 to those of civet ACE2, or by altering S-protein residues 479 and 487 to residues conserved during the 2002-2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS-CoV to human cells, and provide insight into the severity of the 2002-2003 SARS epidemic. © 2005 European Molecular Biology Organization | All Rights Reserved.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/emboj/index.htmlen_HK
dc.relation.ispartofEMBO Journalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAngiotensin-converting enzyme 2en_HK
dc.subjectCoronavirusen_HK
dc.subjectSevere acute respiratory syndromeen_HK
dc.subjectViral adaptationen_HK
dc.titleReceptor and viral determinants of SARS-coronavirus adaptation to human ACE2en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0261-4189&volume=24&issue=8&spage=1634&epage=1643&date=2005&atitle=Receptor+and+viral+determinants+of+SARS-coronavirus+adaptation+to+human+ACE2en_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1038/sj.emboj.7600640en_HK
dc.identifier.pmid15791205-
dc.identifier.pmcidPMC1142572en_HK
dc.identifier.scopuseid_2-s2.0-20844452721en_HK
dc.identifier.hkuros110243-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20844452721&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1634en_HK
dc.identifier.epage1643en_HK
dc.identifier.isiWOS:000228943900013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, W=7501793921en_HK
dc.identifier.scopusauthoridZhang, C=7405496386en_HK
dc.identifier.scopusauthoridSui, J=7006812991en_HK
dc.identifier.scopusauthoridKuhn, JH=7201530949en_HK
dc.identifier.scopusauthoridMoore, MJ=7404469988en_HK
dc.identifier.scopusauthoridLuo, S=55254453500en_HK
dc.identifier.scopusauthoridWong, SK=36912447800en_HK
dc.identifier.scopusauthoridHuang, IC=8574051200en_HK
dc.identifier.scopusauthoridXu, K=35228266300en_HK
dc.identifier.scopusauthoridVasilieva, N=7003442041en_HK
dc.identifier.scopusauthoridMurakami, A=7202094217en_HK
dc.identifier.scopusauthoridHe, Y=35285643800en_HK
dc.identifier.scopusauthoridMarasco, WA=35502112300en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridChoe, H=7103055410en_HK
dc.identifier.scopusauthoridFarzan, M=7003535041en_HK
dc.identifier.citeulike139446-

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