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Article: Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome

TitleEvaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome
Authors
Issue Date2004
PublisherAmerican Society for Microbiology.
Citation
Clinical And Diagnostic Laboratory Immunology, 2004, v. 11 n. 4, p. 699-703 How to Cite?
AbstractA newly developed severe acute respiratory syndrome (SARS)-specific enzyme-linked immunosorbent assay (ELISA) was further validated to confirm cutoff values and evaluate its diagnostic performance with clinical samples. In parallel, an immunochromatographic test was also evaluated. A total of 227 clinical serum specimens collected from SARS patients were used in the study, together with 385 samples from healthy donors. By use of an immunofluorescent (IF) test as the "gold standard," both the ELISA and the immunochromatographic test were able to detect immunoglobulin G antibodies to SARS not only from late-convalescent-stage samples (>21 days from the onset of clinical symptoms), as previously established, but also from early-acute-phase samples (1 to 10 days from onset). The ELISA, using an optical density (OD) of 0.25 as its cutoff value, produced the best sensitivity while maintaining high specificity. It detected SARS-specific antibodies in 58, 70, 75, and 95%, respectively, of the four groups of samples collected from patients 1 to 10 days, 11 to 20 days, 21 to 30 days, and more than 30 days after the onset of clinical symptoms. Similarly, the immunochromatographic test detected SARS-specific antibodies in 55, 68, 81, and 79% of the four groups, respectively. The overall specificities for the ELISA and the rapid test were 99.5 and 97.7%, respectively. Although the positive correlation observed between the ELISA OD values and the IF titers was moderate (r = 9.6915; P < 0.001), the detection rates of both the ELISA and the rapid test were found well in agreement with the IF titers.
Persistent Identifierhttp://hdl.handle.net/10722/49157
ISSN
2007 Impact Factor: 2.511
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGuan, Men_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorKwan, SWen_HK
dc.contributor.authorLam, SYen_HK
dc.contributor.authorPang, CMen_HK
dc.contributor.authorChu, KWen_HK
dc.contributor.authorChan, KMen_HK
dc.contributor.authorChen, HYen_HK
dc.contributor.authorPhuah, EBen_HK
dc.contributor.authorWong, CJen_HK
dc.date.accessioned2008-06-12T06:35:42Z-
dc.date.available2008-06-12T06:35:42Z-
dc.date.issued2004en_HK
dc.identifier.citationClinical And Diagnostic Laboratory Immunology, 2004, v. 11 n. 4, p. 699-703en_HK
dc.identifier.issn1071-412Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49157-
dc.description.abstractA newly developed severe acute respiratory syndrome (SARS)-specific enzyme-linked immunosorbent assay (ELISA) was further validated to confirm cutoff values and evaluate its diagnostic performance with clinical samples. In parallel, an immunochromatographic test was also evaluated. A total of 227 clinical serum specimens collected from SARS patients were used in the study, together with 385 samples from healthy donors. By use of an immunofluorescent (IF) test as the "gold standard," both the ELISA and the immunochromatographic test were able to detect immunoglobulin G antibodies to SARS not only from late-convalescent-stage samples (>21 days from the onset of clinical symptoms), as previously established, but also from early-acute-phase samples (1 to 10 days from onset). The ELISA, using an optical density (OD) of 0.25 as its cutoff value, produced the best sensitivity while maintaining high specificity. It detected SARS-specific antibodies in 58, 70, 75, and 95%, respectively, of the four groups of samples collected from patients 1 to 10 days, 11 to 20 days, 21 to 30 days, and more than 30 days after the onset of clinical symptoms. Similarly, the immunochromatographic test detected SARS-specific antibodies in 55, 68, 81, and 79% of the four groups, respectively. The overall specificities for the ELISA and the rapid test were 99.5 and 97.7%, respectively. Although the positive correlation observed between the ELISA OD values and the IF titers was moderate (r = 9.6915; P < 0.001), the detection rates of both the ELISA and the rapid test were found well in agreement with the IF titers.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofClinical and Diagnostic Laboratory Immunologyen_HK
dc.rightsClinical and Diagnostic Laboratory Immunology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Clinical and Diagnostic Laboratory Immunology, 2004, v. 11 n. 4, p. 699-703en_HK
dc.subject.meshChromatography - methodsen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methodsen_HK
dc.subject.meshSerologic Tests - standardsen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - blood - diagnosisen_HK
dc.subject.meshAntibodies, Viral - blooden_HK
dc.titleEvaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndromeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1071-412X&volume=11&issue=4&spage=699&epage=703&date=2004&atitle=Evaluation+and+validation+of+an+enzyme-linked+immunosorbent+assay+and+an+immunochromatographic+test+for+serological+diagnosis+of+severe+acute+respiratory+syndromeen_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/CDLI.11.4.699-703.2004en_HK
dc.identifier.pmid15242944-
dc.identifier.pmcidPMC440612en_HK
dc.identifier.scopuseid_2-s2.0-3242700575en_HK
dc.identifier.hkuros109471-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3242700575&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue4en_HK
dc.identifier.spage699en_HK
dc.identifier.epage703en_HK
dc.identifier.isiWOS:000222786000011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuan, M=17343647900en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridKwan, SW=35887222800en_HK
dc.identifier.scopusauthoridLam, SY=23073866700en_HK
dc.identifier.scopusauthoridPang, CM=7201425130en_HK
dc.identifier.scopusauthoridChu, KW=8439274900en_HK
dc.identifier.scopusauthoridChan, KM=36915726300en_HK
dc.identifier.scopusauthoridChen, HY=7501609850en_HK
dc.identifier.scopusauthoridPhuah, EB=6507243341en_HK
dc.identifier.scopusauthoridWong, CJ=7404954478en_HK

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