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Article: Regulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts

TitleRegulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts
Authors
Issue Date2005
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2005, v. 79 n. 3, p. 1724-1733 How to Cite?
AbstractThe Epstein-Barr virus (EBV) BamHI-A rightward transcripts, or BARTs, are a family of mRNAs expressed in all EBV latency programs, including EBV-infected B cells in healthy carriers. Despite their ubiquitous expression, the regulation and biological function of BARTs are still unclear. In this study, the BART 5′ termini were characterized by using a procedure that selects capped, full-length mRNAs. Two TATA-less promoter regions, designated P1 and P2, were mapped. P1 had relatively high basal activity in both epithelial and B cells, whereas P2 exhibited higher activity in epithelial cells. Upon EBV infection of B cells, transcription from P1 was detected soon after infection, while expression from P2 was delayed. Promoter-reporter assays in transiently transfected cells revealed that P1 and P2 were differentially regulated. Interferon regulatory factor 7 (IRF7) and IRF5 negatively regulated P1 activity. c-Myc and C/EBP family members positively regulated P2. Regulation of P2 by C/EBPs was characterized by electrophoretic mobility shift assay, chromatin immunoprecipitation, and reporter assays. More-abundant BART expression in epithelial cells correlated with the relative expression of positive and negative regulators in these cells. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/49156
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Hen_HK
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorWu, FYen_HK
dc.contributor.authorLiao, Gen_HK
dc.contributor.authorHuttFletcher, Len_HK
dc.contributor.authorHayward, SDen_HK
dc.date.accessioned2008-06-12T06:35:41Z-
dc.date.available2008-06-12T06:35:41Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Virology, 2005, v. 79 n. 3, p. 1724-1733en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49156-
dc.description.abstractThe Epstein-Barr virus (EBV) BamHI-A rightward transcripts, or BARTs, are a family of mRNAs expressed in all EBV latency programs, including EBV-infected B cells in healthy carriers. Despite their ubiquitous expression, the regulation and biological function of BARTs are still unclear. In this study, the BART 5′ termini were characterized by using a procedure that selects capped, full-length mRNAs. Two TATA-less promoter regions, designated P1 and P2, were mapped. P1 had relatively high basal activity in both epithelial and B cells, whereas P2 exhibited higher activity in epithelial cells. Upon EBV infection of B cells, transcription from P1 was detected soon after infection, while expression from P2 was delayed. Promoter-reporter assays in transiently transfected cells revealed that P1 and P2 were differentially regulated. Interferon regulatory factor 7 (IRF7) and IRF5 negatively regulated P1 activity. c-Myc and C/EBP family members positively regulated P2. Regulation of P2 by C/EBPs was characterized by electrophoretic mobility shift assay, chromatin immunoprecipitation, and reporter assays. More-abundant BART expression in epithelial cells correlated with the relative expression of positive and negative regulators in these cells. Copyright © 2005, American Society for Microbiology. All Rights Reserved.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2005, v. 79 n. 3, p. 1724-1733en_HK
dc.subject.meshDeoxyribonuclease BamHI - metabolismen_HK
dc.subject.meshGene Expression Regulation, Viralen_HK
dc.subject.meshHerpesvirus 4, Human - genetics - metabolism - pathogenicityen_HK
dc.subject.meshPromoter Regions (Genetics)en_HK
dc.subject.meshRestriction Mappingen_HK
dc.titleRegulation of expression of the Epstein-Barr virus BamHI-A rightward transcriptsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=79&issue=3&spage=1724&epage=1733&date=2005&atitle=Regulation+of+expression+of+the+Epstein-Barr+virus+BamHI-A+rightward+transcriptsen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JVI.79.3.1724-1733.2005en_HK
dc.identifier.pmid15650197en_HK
dc.identifier.pmcidPMC544122en_HK
dc.identifier.scopuseid_2-s2.0-13744259225en_HK
dc.identifier.hkuros109263-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13744259225&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1724en_HK
dc.identifier.epage1733en_HK
dc.identifier.isiWOS:000226634300038-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridHuang, J=34770766800en_HK
dc.identifier.scopusauthoridWu, FY=36991458700en_HK
dc.identifier.scopusauthoridLiao, G=7102949054en_HK
dc.identifier.scopusauthoridHuttFletcher, L=7004909823en_HK
dc.identifier.scopusauthoridHayward, SD=7102776214en_HK

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