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Article: Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004

TitleLethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004
Authors
Issue Date2005
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2005, v. 79 n. 4, p. 2191-2198 How to Cite?
AbstractThe 2004 outbreaks of H5N1 influenza viruses in Vietnam and Thailand were highly lethal to humans and to poultry; therefore, newly emerging avian influenza A viruses pose a continued threat, not only to avian species but also to humans. We studied the pathogenicity of four human and nine avian H5N1/04 influenza viruses in ferrets (an excellent model for influenza studies). All four human isolates were fatal to intranasally inoculated ferrets. The human isolate A/Vietnam/1203/04 (H5N1) was the most pathogenic isolate; the severity of disease was associated with a broad tissue tropism and high virus titers in multiple organs, including the brain. High fever, weight loss, anorexia, extreme lethargy, and diarrhea were observed. Two avian H5N1/04 isolates were as pathogenic as the human viruses, causing lethal systemic infections in ferrets. Seven of nine H5N1/04 viruses isolated from avian species caused mild infections, with virus replication restricted to the upper respiratory tract. All chicken isolates were nonlethal to ferrets. A sequence analysis revealed polybasic amino acids in the hemagglutinin connecting peptides of all H5N1/04 viruses, indicating that multiple molecular differences in other genes are important for a high level of virulence. Interestingly, the human A/Vietnam/ 1203/04 isolate had a lysine substitution at position 627 of PB2 and had one to eight amino acid changes in all gene products except that of the M1 gene, unlike the A/chicken/Vietnam/C58/04 and A/quail/Vietnam/36/04 viruses. Our results indicate that viruses that are lethal to mammals are circulating among birds in Asia and suggest that pathogenicity in ferrets, and perhaps humans, reflects a complex combination of different residues rather than a single amino acid difference.
Persistent Identifierhttp://hdl.handle.net/10722/49152
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
References
Errata

 

DC FieldValueLanguage
dc.contributor.authorGovorkova, EAen_HK
dc.contributor.authorRehg, JEen_HK
dc.contributor.authorKrauss, Sen_HK
dc.contributor.authorYen, HLen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, Men_HK
dc.contributor.authorNguyen, TDen_HK
dc.contributor.authorHanh, THen_HK
dc.contributor.authorPuthavathana, Pen_HK
dc.contributor.authorLong, HTen_HK
dc.contributor.authorBuranathai, Cen_HK
dc.contributor.authorLim, Wen_HK
dc.contributor.authorWebster, RGen_HK
dc.contributor.authorHoffmann, Een_HK
dc.date.accessioned2008-06-12T06:35:35Z-
dc.date.available2008-06-12T06:35:35Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Virology, 2005, v. 79 n. 4, p. 2191-2198en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49152-
dc.description.abstractThe 2004 outbreaks of H5N1 influenza viruses in Vietnam and Thailand were highly lethal to humans and to poultry; therefore, newly emerging avian influenza A viruses pose a continued threat, not only to avian species but also to humans. We studied the pathogenicity of four human and nine avian H5N1/04 influenza viruses in ferrets (an excellent model for influenza studies). All four human isolates were fatal to intranasally inoculated ferrets. The human isolate A/Vietnam/1203/04 (H5N1) was the most pathogenic isolate; the severity of disease was associated with a broad tissue tropism and high virus titers in multiple organs, including the brain. High fever, weight loss, anorexia, extreme lethargy, and diarrhea were observed. Two avian H5N1/04 isolates were as pathogenic as the human viruses, causing lethal systemic infections in ferrets. Seven of nine H5N1/04 viruses isolated from avian species caused mild infections, with virus replication restricted to the upper respiratory tract. All chicken isolates were nonlethal to ferrets. A sequence analysis revealed polybasic amino acids in the hemagglutinin connecting peptides of all H5N1/04 viruses, indicating that multiple molecular differences in other genes are important for a high level of virulence. Interestingly, the human A/Vietnam/ 1203/04 isolate had a lysine substitution at position 627 of PB2 and had one to eight amino acid changes in all gene products except that of the M1 gene, unlike the A/chicken/Vietnam/C58/04 and A/quail/Vietnam/36/04 viruses. Our results indicate that viruses that are lethal to mammals are circulating among birds in Asia and suggest that pathogenicity in ferrets, and perhaps humans, reflects a complex combination of different residues rather than a single amino acid difference.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2005, v. 79 n. 4, p. 2191-2198en_HK
dc.subject.meshHemagglutinin Glycoproteins, Influenza Virus - chemistry - geneticsen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtypeen_HK
dc.subject.meshInfluenza A virus - classification - isolation & purification - pathogenicityen_HK
dc.subject.meshInfluenza, Human - mortality - pathology - virologyen_HK
dc.subject.meshOrthomyxoviridae - pathogenicityen_HK
dc.titleLethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=79&issue=4&spage=2191&epage=2198&date=2005&atitle=Lethality+to+ferrets+of+H5N1+influenza+viruses+isolated+from+humans+and+poultry+in+2004en_HK
dc.identifier.emailYen, HL: hyen@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_HK
dc.identifier.authorityYen, HL=rp00304en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, M=rp00410en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JVI.79.4.2191-2198.2005en_HK
dc.identifier.pmid15681421-
dc.identifier.pmcidPMC546577en_HK
dc.identifier.scopuseid_2-s2.0-13444292912en_HK
dc.identifier.hkuros106411-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13444292912&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue4en_HK
dc.identifier.spage2191en_HK
dc.identifier.epage2198en_HK
dc.identifier.isiWOS:000226772100022-
dc.publisher.placeUnited Statesen_HK
dc.relation.erratumdoi:10.1128/JVI.00686-06-
dc.identifier.scopusauthoridGovorkova, EA=7003837718en_HK
dc.identifier.scopusauthoridRehg, JE=7004835777en_HK
dc.identifier.scopusauthoridKrauss, S=7102769210en_HK
dc.identifier.scopusauthoridYen, HL=7102476668en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, M=7005486823en_HK
dc.identifier.scopusauthoridNguyen, TD=25227791100en_HK
dc.identifier.scopusauthoridHanh, TH=16636566700en_HK
dc.identifier.scopusauthoridPuthavathana, P=7004642707en_HK
dc.identifier.scopusauthoridLong, HT=7102434129en_HK
dc.identifier.scopusauthoridBuranathai, C=6507522489en_HK
dc.identifier.scopusauthoridLim, W=7202378277en_HK
dc.identifier.scopusauthoridWebster, RG=36048363100en_HK
dc.identifier.scopusauthoridHoffmann, E=7201369718en_HK

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