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Article: Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
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TitleCharacterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
 
AuthorsWoo, PCY2 2
Lau, SKP2 2
Chu, CM3
Chan, KH2
Tsoi, HW2
Huang, Y2
Wong, BHL2
Poon, RWS2
Cai, JJ2
Luk, WK1
Poon, LLM2 2
Wong, SSY2 2
Guan, Y2 2
Peiris, JSM2 2
Yuen, KY2 2 2
 
Issue Date2005
 
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
CitationJournal Of Virology, 2005, v. 79 n. 2, p. 884-895 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.2.884-895.2005
 
AbstractDespite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.
 
ISSN0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
DOIhttp://dx.doi.org/10.1128/JVI.79.2.884-895.2005
 
PubMed Central IDPMC538593
 
ISI Accession Number IDWOS:000226149700024
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWoo, PCY
 
dc.contributor.authorLau, SKP
 
dc.contributor.authorChu, CM
 
dc.contributor.authorChan, KH
 
dc.contributor.authorTsoi, HW
 
dc.contributor.authorHuang, Y
 
dc.contributor.authorWong, BHL
 
dc.contributor.authorPoon, RWS
 
dc.contributor.authorCai, JJ
 
dc.contributor.authorLuk, WK
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorWong, SSY
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2008-06-12T06:35:28Z
 
dc.date.available2008-06-12T06:35:28Z
 
dc.date.issued2005
 
dc.description.abstractDespite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.
 
dc.description.naturepublished_or_final_version
 
dc.format.extent386 bytes
 
dc.format.mimetypetext/html
 
dc.identifier.citationJournal Of Virology, 2005, v. 79 n. 2, p. 884-895 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.2.884-895.2005
 
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.79.2.884-895.2005
 
dc.identifier.epage895
 
dc.identifier.hkuros100255
 
dc.identifier.hkuros114695
 
dc.identifier.isiWOS:000226149700024
 
dc.identifier.issn0022-538X
2012 Impact Factor: 5.076
2012 SCImago Journal Rankings: 2.559
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC538593
 
dc.identifier.pmid15613317
 
dc.identifier.scopuseid_2-s2.0-19944427959
 
dc.identifier.spage884
 
dc.identifier.urihttp://hdl.handle.net/10722/49146
 
dc.identifier.volume79
 
dc.languageeng
 
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2005, v. 79 n. 2, p. 884-895
 
dc.subject.meshCoronavirus - classification - genetics
 
dc.subject.meshGenome, Viral
 
dc.subject.meshPneumonia, Viral - virology
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
 
dc.subject.meshSequence Analysis, DNA
 
dc.titleCharacterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
 
dc.typeArticle
 
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Author Affiliations
  1. Tseung Kwan O Hospital
  2. The University of Hong Kong
  3. United Christian Hospital Hong Kong