Article: Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia

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TitleCharacterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
AuthorsWoo, PCY2
Lau, SKP2
Chu, CM3
Chan, KH2
Tsoi, HW2
Huang, Y2
Wong, BHL2
Poon, RWS2
Cai, JJ2
Luk, WK1
Poon, LLM2
Wong, SSY2
Guan, Y2
Peiris, JSM2
Yuen, KY2
Issue Date2005
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
CitationJournal Of Virology, 2005, v. 79 n. 2, p. 884-895 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.2.884-895.2005
AbstractDespite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.
ISSN0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
DOIhttp://dx.doi.org/10.1128/JVI.79.2.884-895.2005
ISI Accession Number IDWOS:000226149700024
PubMed Central IDPMC538593
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWoo, PCY
dc.contributor.authorLau, SKP
dc.contributor.authorChu, CM
dc.contributor.authorChan, KH
dc.contributor.authorTsoi, HW
dc.contributor.authorHuang, Y
dc.contributor.authorWong, BHL
dc.contributor.authorPoon, RWS
dc.contributor.authorCai, JJ
dc.contributor.authorLuk, WK
dc.contributor.authorPoon, LLM
dc.contributor.authorWong, SSY
dc.contributor.authorGuan, Y
dc.contributor.authorPeiris, JSM
dc.contributor.authorYuen, KY
dc.date.accessioned2008-06-12T06:35:28Z
dc.date.available2008-06-12T06:35:28Z
dc.date.issued2005
dc.description.abstractDespite extensive laboratory investigations in patients with respiratory tract infections, no microbiological cause can be identified in a significant proportion of patients. In the past 3 years, several novel respiratory viruses, including human metapneumovirus, severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV), and human coronavirus NL63, were discovered. Here we report the discovery of another novel coronavirus, coronavirus HKU1 (CoV-HKU1), from a 71-year-old man with pneumonia who had just returned from Shenzhen, China. Quantitative reverse transcription-PCR showed that the amount of CoV-HKU1 RNA was 8.5 to 9.6 × 106 copies per ml in his nasopharyngeal aspirates (NPAs) during the first week of the illness and dropped progressively to undetectable levels in subsequent weeks. He developed increasing serum levels of specific antibodies against the recombinant nucleocapsid protein of CoV-HKU1, with immunoglobulin M (IgM) titers of 1:20, 1:40, and 1:80 and IgG titers of <1:1,000, 1:2,000, and 1:8,000 in the first, second and fourth weeks of the illness, respectively. Isolation of the virus by using various cell lines, mixed neuron-glia culture, and intracerebral inoculation of suckling mice was unsuccessful. The complete genome sequence of CoV-HKU1 is a 29,926-nucleotide, polyadenylated RNA, with G+C content of 32%, the lowest among all known coronaviruses with available genome sequence. Phylogenetic analysis reveals that CoV-HKU1 is a new group 2 coronavirus. Screening of 400 NPAs, negative for SARS-CoV, from patients with respiratory illness during the SARS period identified the presence of CoV-HKU1 RNA in an additional specimen, with a viral load of 1.13 × 106 copies per ml, from a 35-year-old woman with pneumonia. Our data support the existence of a novel group 2 coronavirus associated with pneumonia in humans.
dc.description.naturepublished_or_final_version
dc.format.extent386 bytes
dc.format.mimetypetext/html
dc.identifier.citationJournal Of Virology, 2005, v. 79 n. 2, p. 884-895 [How to Cite?]
DOI: http://dx.doi.org/10.1128/JVI.79.2.884-895.2005
dc.identifier.doihttp://dx.doi.org/10.1128/JVI.79.2.884-895.2005
dc.identifier.epage895
dc.identifier.hkuros100255
dc.identifier.hkuros114695
dc.identifier.isiWOS:000226149700024
dc.identifier.issn0022-538X
2011 Impact Factor: 5.402
2011 SCImago Journal Rankings: 0.745
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmcidPMC538593
dc.identifier.pmid15613317
dc.identifier.scopuseid_2-s2.0-19944427959
dc.identifier.spage884
dc.identifier.urihttp://hdl.handle.net/10722/49146
dc.identifier.volume79
dc.languageeng
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Virology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2005, v. 79 n. 2, p. 884-895
dc.subject.meshCoronavirus - classification - genetics
dc.subject.meshGenome, Viral
dc.subject.meshPneumonia, Viral - virology
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction
dc.subject.meshSequence Analysis, DNA
dc.titleCharacterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia
dc.typeArticle
Author Affiliations
  1. Tseung Kwan O Hospital
  2. The University of Hong Kong
  3. United Christian Hospital Hong Kong