Links for fulltext
     (May Require Subscription)
Supplementary

Article: False-positive results in a recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by western blotting with recombinant SARS-CoV spike polypeptide

TitleFalse-positive results in a recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by western blotting with recombinant SARS-CoV spike polypeptide
Authors
Issue Date2004
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2004, v. 42 n. 12, p. 5885-5888 How to Cite?
AbstractUsing paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.
Persistent Identifierhttp://hdl.handle.net/10722/49144
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWong, BHLen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorHui, WTen_HK
dc.contributor.authorKwan, GSWen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorCouch, RBen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:35:25Z-
dc.date.available2008-06-12T06:35:25Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2004, v. 42 n. 12, p. 5885-5888en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49144-
dc.description.abstractUsing paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of clinical microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of clinical microbiology, 2004, v. 42 n. 12, p. 5885-8en_HK
dc.subject.meshMembrane Glycoproteins - genetics - immunologyen_HK
dc.subject.meshNucleocapsid Proteins - genetics - immunologyen_HK
dc.subject.meshRecombinant Proteins - immunologyen_HK
dc.subject.meshSARS Virus - immunologyen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - diagnosis - virologyen_HK
dc.titleFalse-positive results in a recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by western blotting with recombinant SARS-CoV spike polypeptideen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=42&issue=12&spage=5885&epage=8&date=2004&atitle=False-positive+results+in+a+recombinant+severe+acute+respiratory+syndrome-associated+coronavirus+(SARS-CoV)+nucleocapsid+enzyme-linked+immunosorbent+assay+due+to+HCoV-OC43+and+HCoV-229E+rectified+by+Western+blotting+with+recombinant+SARS-CoV+spike+polypepen_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.42.12.5885-5888.2004en_HK
dc.identifier.pmid15583332-
dc.identifier.pmcidPMC535232en_HK
dc.identifier.scopuseid_2-s2.0-10844237862en_HK
dc.identifier.hkuros100213-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10844237862&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue12en_HK
dc.identifier.spage5885en_HK
dc.identifier.epage5888en_HK
dc.identifier.isiWOS:000226035800071-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWong, BHL=7402023413en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridHui, WT=7103196480en_HK
dc.identifier.scopusauthoridKwan, GSW=36658582800en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridCouch, RB=7102611225en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats