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Article: Evolutionary dynamics of insertion sequences in Helicobacter pylori

TitleEvolutionary dynamics of insertion sequences in Helicobacter pylori
Authors
Issue Date2004
PublisherAmerican Society for Microbiology.
Citation
Journal Of Bacteriology, 2004, v. 186 n. 22, p. 7508-7520 How to Cite?
AbstractProkaryotic insertion sequence (IS) elements behave like parasites in terms of their ability to invade and proliferate in microbial gene pools and like symbionts when they coevolve with their bacterial hosts. Here we investigated the evolutionary history of IS605 and IS607 of Helicobacter pylori, a genetically diverse gastric pathogen. These elements contain unrelated transposase genes (orfA) and also a homolog of the Salmonella virulence gene gipA (orfB). A total of 488 East Asian, Indian, Peruvian, and Spanish isolates were screened, and 18 and 14% of them harbored IS605 and IS607, respectively. IS605 nucleotide sequence analysis (n = 42) revealed geographic subdivisions similar to those of H. pylori; the geographic subdivision was blurred, however, due in part to homologous recombination, as indicated by split decomposition and homoplasy tests (homoplasy ratio, 0.56). In contrast, the IS607 populations (n = 44) showed strong geographic subdivisions with less homologous recombination (homoplasy ratio, 0.2). Diversifying selection (ratio of nonsynonymous change to synonymous change, ≫1) was evident in 1∼5% of the IS605 orfA codons analyzed but not in the IS607 orfA codons. Diversifying selection was also evident in ∼2% of the IS605 orfB and ∼10% of the IS607 orfB codons analyzed. We suggest that the evolution of these elements reflects selection for optimal transposition activity in the case of IS605 orfA and for interactions between the OrfB proteins and other cellular constituents that potentially contribute to bacterial fitness. Taken together, similarities in IS elements and H. pylori population genetic structures and evidence of adaptive evolution in IS elements suggest that there is coevolution between these elements and their bacterial hosts.
Persistent Identifierhttp://hdl.handle.net/10722/49140
ISSN
2015 Impact Factor: 3.198
2015 SCImago Journal Rankings: 2.216
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKalia, Aen_HK
dc.contributor.authorMukhopadhyay, AKen_HK
dc.contributor.authorDailide, Gen_HK
dc.contributor.authorIto, Yen_HK
dc.contributor.authorAzuma, Ten_HK
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorBerg, DEen_HK
dc.date.accessioned2008-06-12T06:35:17Z-
dc.date.available2008-06-12T06:35:17Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Bacteriology, 2004, v. 186 n. 22, p. 7508-7520en_HK
dc.identifier.issn0021-9193en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49140-
dc.description.abstractProkaryotic insertion sequence (IS) elements behave like parasites in terms of their ability to invade and proliferate in microbial gene pools and like symbionts when they coevolve with their bacterial hosts. Here we investigated the evolutionary history of IS605 and IS607 of Helicobacter pylori, a genetically diverse gastric pathogen. These elements contain unrelated transposase genes (orfA) and also a homolog of the Salmonella virulence gene gipA (orfB). A total of 488 East Asian, Indian, Peruvian, and Spanish isolates were screened, and 18 and 14% of them harbored IS605 and IS607, respectively. IS605 nucleotide sequence analysis (n = 42) revealed geographic subdivisions similar to those of H. pylori; the geographic subdivision was blurred, however, due in part to homologous recombination, as indicated by split decomposition and homoplasy tests (homoplasy ratio, 0.56). In contrast, the IS607 populations (n = 44) showed strong geographic subdivisions with less homologous recombination (homoplasy ratio, 0.2). Diversifying selection (ratio of nonsynonymous change to synonymous change, ≫1) was evident in 1∼5% of the IS605 orfA codons analyzed but not in the IS607 orfA codons. Diversifying selection was also evident in ∼2% of the IS605 orfB and ∼10% of the IS607 orfB codons analyzed. We suggest that the evolution of these elements reflects selection for optimal transposition activity in the case of IS605 orfA and for interactions between the OrfB proteins and other cellular constituents that potentially contribute to bacterial fitness. Taken together, similarities in IS elements and H. pylori population genetic structures and evidence of adaptive evolution in IS elements suggest that there is coevolution between these elements and their bacterial hosts.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Bacteriologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Bacteriology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Bacteriology, 2004, v. 186 n. 22, p. 7508-7520en_HK
dc.subject.meshDNA Transposable Elements - geneticsen_HK
dc.subject.meshEvolution, Molecularen_HK
dc.subject.meshHelicobacter pylori - geneticsen_HK
dc.subject.meshSelection (Genetics)en_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleEvolutionary dynamics of insertion sequences in Helicobacter pylorien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9193&volume=186&issue=22&spage=7508&epage=7520&date=2004&atitle=Evolutionary+dynamics+of+insertion+sequences+in+Helicobacter+pylorien_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JB.186.22.7508-7520.2004en_HK
dc.identifier.pmid15516562en_HK
dc.identifier.pmcidPMC524885en_HK
dc.identifier.scopuseid_2-s2.0-7744220354en_HK
dc.identifier.hkuros99180-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-7744220354&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume186en_HK
dc.identifier.issue22en_HK
dc.identifier.spage7508en_HK
dc.identifier.epage7520en_HK
dc.identifier.isiWOS:000224973500006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKalia, A=7005451652en_HK
dc.identifier.scopusauthoridMukhopadhyay, AK=7201816905en_HK
dc.identifier.scopusauthoridDailide, G=6506687791en_HK
dc.identifier.scopusauthoridIto, Y=36559261800en_HK
dc.identifier.scopusauthoridAzuma, T=7201514361en_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridBerg, DE=7202401139en_HK
dc.identifier.citeulike6607532-

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