File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Bikunin present in human peritoneal fluid is in part derived from the interaction of serum with peritoneal mesothelial cells

TitleBikunin present in human peritoneal fluid is in part derived from the interaction of serum with peritoneal mesothelial cells
Authors
Issue Date1998
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1998, v. 153 n. 4, p. 1267-1276 How to Cite?
AbstractWe recently reported that peritoneal fluid mainly contains two proteoglycans; one is the interstitial proteoglycan referred to as decorin, and the other an uncharacterized small chondroitin sulfate proteoglycan. In the present study, we have used a two-step process to isolate the small chondroitin sulfate proteoglycan free of decorin. The purified molecule ran as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with apparent molecular mass 50 kd made up of a chondroitin-4-sulfate glycosaminoglycan chain and a 30-kd core protein. NH 2-terminal analysis of the core protein showed significant sequence homology with bikunin, a component of the human inter-α-trypsin inhibitor (IαI) family. A Western blot analysis using anti-human inter-α-trypsin inhibitor confirmed the identity of the small chondroitin sulfate proteoglycan as bikunin, and a trypsin inhibitor counterstain assay confirmed its anti-trypsin activity. Examination of serum from patients receiving continuous peritoneal dialysis suggests that free bikunin in peritoneal fluid may be the result of leakage of serum proteins into the peritoneum. Our findings further show that the interaction of serum with peritoneal mesothelial cells offers a new and novel explanation for the presence of bikunin in peritoneal fluid.
Persistent Identifierhttp://hdl.handle.net/10722/49099
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThomas, GJen_HK
dc.contributor.authorYung, Sen_HK
dc.contributor.authorDavies, Men_HK
dc.date.accessioned2008-06-12T06:34:24Z-
dc.date.available2008-06-12T06:34:24Z-
dc.date.issued1998en_HK
dc.identifier.citationAmerican Journal Of Pathology, 1998, v. 153 n. 4, p. 1267-1276en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49099-
dc.description.abstractWe recently reported that peritoneal fluid mainly contains two proteoglycans; one is the interstitial proteoglycan referred to as decorin, and the other an uncharacterized small chondroitin sulfate proteoglycan. In the present study, we have used a two-step process to isolate the small chondroitin sulfate proteoglycan free of decorin. The purified molecule ran as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with apparent molecular mass 50 kd made up of a chondroitin-4-sulfate glycosaminoglycan chain and a 30-kd core protein. NH 2-terminal analysis of the core protein showed significant sequence homology with bikunin, a component of the human inter-α-trypsin inhibitor (IαI) family. A Western blot analysis using anti-human inter-α-trypsin inhibitor confirmed the identity of the small chondroitin sulfate proteoglycan as bikunin, and a trypsin inhibitor counterstain assay confirmed its anti-trypsin activity. Examination of serum from patients receiving continuous peritoneal dialysis suggests that free bikunin in peritoneal fluid may be the result of leakage of serum proteins into the peritoneum. Our findings further show that the interaction of serum with peritoneal mesothelial cells offers a new and novel explanation for the presence of bikunin in peritoneal fluid.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshHerpesvirus 4, Human - geneticsen_HK
dc.subject.meshLymphoma, B-Cell - etiology - virologyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshPromoter Regions (Genetics)en_HK
dc.subject.meshBurkitt Lymphoma - virologyen_HK
dc.titleBikunin present in human peritoneal fluid is in part derived from the interaction of serum with peritoneal mesothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9440&volume=153&issue=4&spage=1267&epage=1276&date=1998&atitle=Bikunin+is+a+major+proteoglycan+of+the+peritoneum+and+is+secreted+into+the+CAPD+fluiden_HK
dc.identifier.emailYung, S:ssyyung@hku.hken_HK
dc.identifier.authorityYung, S=rp00455en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9777958-
dc.identifier.pmcidPMC1853064en_HK
dc.identifier.scopuseid_2-s2.0-0031695122en_HK
dc.identifier.hkuros46247-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031695122&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume153en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1267en_HK
dc.identifier.epage1276en_HK
dc.identifier.isiWOS:000076364900028-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridThomas, GJ=35464458200en_HK
dc.identifier.scopusauthoridYung, S=22636568800en_HK
dc.identifier.scopusauthoridDavies, M=7404207291en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats