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Article: Glycoproteins present in human follicular fluid that inhibit the zona-binding capacity of spermatozoa

TitleGlycoproteins present in human follicular fluid that inhibit the zona-binding capacity of spermatozoa
Authors
KeywordsFollicular fluid
Glycoprotein
Hemizona binding assay
Spermatozoa
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1998, v. 13 n. 9, p. 2541-2547 How to Cite?
AbstractPrevious studies have suggested that human follicular fluid contains factors that reduce the zona-binding capacity of spermatozoa. The present study provides further evidence of the existence of such factors. Using the hemizona binding assay (HZA), we have shown that the inhibitory effect of human follicular fluid on the zona-binding capacity of spermatozoa is concentration-dependent, an inhibitory effect being detected when the concentration of human follicular fluid was ≤ 10%. A 1% concentration of human follicular fluid did not possess this inhibitory activity. Heating human follicular fluid at 56°C for 30 min did not affect its inhibitory properties; treatment with proteinase-K abolished such inhibition. Human follicular fluid was fractionated sequentially by concanavalin-A affinity chromatography, Mono Q ion-exchange chromatography and Superose-12 gel filtration. The zona binding inhibitory activity resided in the fraction which bound to the lectin and Mono Q column and contained molecules with native molecular weights of 32 and 192 kDa. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis suggested that the 192 kDa glycoprotein was a tetramer, while the 32 kDa glycoprotein remained as a single molecular species under denaturing conditions. We conclude that two glycoproteins were responsible for the zona binding inhibitory activity of human follicular fluid. The physiological role of these factors remains unclear.
Persistent Identifierhttp://hdl.handle.net/10722/49085
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorChiu, CNPen_HK
dc.contributor.authorIp, SMen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2008-06-12T06:34:05Z-
dc.date.available2008-06-12T06:34:05Z-
dc.date.issued1998en_HK
dc.identifier.citationHuman Reproduction, 1998, v. 13 n. 9, p. 2541-2547en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49085-
dc.description.abstractPrevious studies have suggested that human follicular fluid contains factors that reduce the zona-binding capacity of spermatozoa. The present study provides further evidence of the existence of such factors. Using the hemizona binding assay (HZA), we have shown that the inhibitory effect of human follicular fluid on the zona-binding capacity of spermatozoa is concentration-dependent, an inhibitory effect being detected when the concentration of human follicular fluid was ≤ 10%. A 1% concentration of human follicular fluid did not possess this inhibitory activity. Heating human follicular fluid at 56°C for 30 min did not affect its inhibitory properties; treatment with proteinase-K abolished such inhibition. Human follicular fluid was fractionated sequentially by concanavalin-A affinity chromatography, Mono Q ion-exchange chromatography and Superose-12 gel filtration. The zona binding inhibitory activity resided in the fraction which bound to the lectin and Mono Q column and contained molecules with native molecular weights of 32 and 192 kDa. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis suggested that the 192 kDa glycoprotein was a tetramer, while the 32 kDa glycoprotein remained as a single molecular species under denaturing conditions. We conclude that two glycoproteins were responsible for the zona binding inhibitory activity of human follicular fluid. The physiological role of these factors remains unclear.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectFollicular fluiden_HK
dc.subjectGlycoproteinen_HK
dc.subjectHemizona binding assayen_HK
dc.subjectSpermatozoaen_HK
dc.titleGlycoproteins present in human follicular fluid that inhibit the zona-binding capacity of spermatozoaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=13&issue=9&spage=2541&epage=2547&date=1998&atitle=Glycoproteins+present+in+human+follicular+fluid+that+inhibit+the+zona-binding+capacity+of+spermatozoaen_HK
dc.identifier.emailChiu, CNP:pchiucn@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, CNP=rp00424en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/humrep/13.9.2541en_HK
dc.identifier.pmid9806280-
dc.identifier.scopuseid_2-s2.0-0031689675en_HK
dc.identifier.hkuros39322-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031689675&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2541en_HK
dc.identifier.epage2547en_HK
dc.identifier.isiWOS:000077290900041-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridChiu, CNP=25959969200en_HK
dc.identifier.scopusauthoridIp, SM=55041321600en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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