File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Intracellular antibody-caspase-mediated cell killing: An approach for application in cancer therapy

TitleIntracellular antibody-caspase-mediated cell killing: An approach for application in cancer therapy
Authors
KeywordsApoptosis
Chromosomal translocation
Gene therapy
Leukemia
Single-chain Fv
Issue Date2000
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings Of The National Academy Of Sciences Of The United States Of America, 2000, v. 97 n. 22, p. 12266-12271 How to Cite?
AbstractAntibodies have been expressed inside cells in an attempt to ablate the function of oncogene products. To make intracellular antibodies more generally applicable and effective in cancer therapy, we have devised a method in which programmed cell death or apoptosis can be triggered by specific antibody-antigen interaction. When intracellular antibodies are linked to caspase 3, the 'executioner' in the apoptosis pathway, and bind to the target antigen, the caspase 3 moieties are self-activated and thereby induce cell killing. We have used this strategy in a model system with two pairs of intracellular antibodies and antigens. In vivo coexpression of an antibody-caspase 3 fusion with its antigenic target induced apoptosis that was specific for antibody, antigen, and active caspase 3. Moreover, the antibody-caspase 3 fusion protein was not toxic to cells in the absence of antigen. Therefore, intracellular antibody-mediated apoptosis should be useful as a specific therapeutic approach for the treatment of cancers, a situation where target cell killing is required.
Persistent Identifierhttp://hdl.handle.net/10722/49040
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, Een_HK
dc.contributor.authorRabbitts, THen_HK
dc.date.accessioned2008-06-12T06:33:07Z-
dc.date.available2008-06-12T06:33:07Z-
dc.date.issued2000en_HK
dc.identifier.citationProceedings Of The National Academy Of Sciences Of The United States Of America, 2000, v. 97 n. 22, p. 12266-12271en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49040-
dc.description.abstractAntibodies have been expressed inside cells in an attempt to ablate the function of oncogene products. To make intracellular antibodies more generally applicable and effective in cancer therapy, we have devised a method in which programmed cell death or apoptosis can be triggered by specific antibody-antigen interaction. When intracellular antibodies are linked to caspase 3, the 'executioner' in the apoptosis pathway, and bind to the target antigen, the caspase 3 moieties are self-activated and thereby induce cell killing. We have used this strategy in a model system with two pairs of intracellular antibodies and antigens. In vivo coexpression of an antibody-caspase 3 fusion with its antigenic target induced apoptosis that was specific for antibody, antigen, and active caspase 3. Moreover, the antibody-caspase 3 fusion protein was not toxic to cells in the absence of antigen. Therefore, intracellular antibody-mediated apoptosis should be useful as a specific therapeutic approach for the treatment of cancers, a situation where target cell killing is required.en_HK
dc.format.extent390 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of Americaen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsNational Academy of Sciences Proceedings. Copyright © National Academy of Sciences.en_HK
dc.subjectApoptosisen_HK
dc.subjectChromosomal translocationen_HK
dc.subjectGene therapyen_HK
dc.subjectLeukemiaen_HK
dc.subjectSingle-chain Fven_HK
dc.titleIntracellular antibody-caspase-mediated cell killing: An approach for application in cancer therapyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=97&issue=22&spage=12266&epage=12271&date=2000&atitle=Intracellular+antibody-caspase-medicated+cell+killing:+an+approach+for+application+in+cancer+therapyen_HK
dc.identifier.emailTse, E:ewctse@hku.hken_HK
dc.identifier.authorityTse, E=rp00471en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1073/pnas.97.22.12266en_HK
dc.identifier.pmid11050246-
dc.identifier.pmcidPMC17330en_HK
dc.identifier.scopuseid_2-s2.0-0034710984en_HK
dc.identifier.hkuros102971-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034710984&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume97en_HK
dc.identifier.issue22en_HK
dc.identifier.spage12266en_HK
dc.identifier.epage12271en_HK
dc.identifier.isiWOS:000090071000101-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, E=7005019454en_HK
dc.identifier.scopusauthoridRabbitts, TH=7103136845en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats