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Article: Transcription factor IIB (TFIIB)-related protein (pBrp), a plant-specific member of the TFIIB-related protein family

TitleTranscription factor IIB (TFIIB)-related protein (pBrp), a plant-specific member of the TFIIB-related protein family
Authors
Issue Date2003
PublisherAmerican Society for Microbiology.
Citation
Molecular and Cellular Biology, 2003, v. 23 n. 9, p. 3274-3286 How to Cite?
AbstractAlthough it is now well documented that metazoans have evolved general transcription factor (GTF) variants to regulate their complex patterns of gene expression, there is so far no information regarding the existence of specific GTFs in plants. Here we report the characterization of a ubiquitously expressed gene that encodes a bona fide novel transcription factor IIB (TFIIB)-related protein in Arabidopsis thaliana. We have shown that this protein is the founding member of a plant-specific TFIIB-related protein family named pBrp (for plant-specific TFIIB-related protein). Surprisingly, in contrast to common GTFs that are localized in the nucleus, the bulk of pBrp proteins are bound to the cytoplasmic face of the plastid envelope, suggesting an organelle-specific function for this novel class of TFIIB-related protein. We show that pBrp proteins harbor conditional proteolytic signals that can target these proteins for rapid turnover by the proteasome-mediated protein degradation pathway. Interestingly, under conditions of proteasome inhibition, pBrp proteins accumulate in the nucleus. Together, our results suggest a possible involvement of these proteins in an intracellular signaling pathway between plastids and the nucleus. Our data provide the first evidence for an organelle-related evolution of the eukaryotic general transcription machinery.
Persistent Identifierhttp://hdl.handle.net/10722/49002
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLagrange, Ten_HK
dc.contributor.authorHakimi, MAen_HK
dc.contributor.authorPontier, Den_HK
dc.contributor.authorCourtois, Fen_HK
dc.contributor.authorAlcaraz, JPen_HK
dc.contributor.authorGrunwald, Den_HK
dc.contributor.authorLam, Een_HK
dc.contributor.authorLerbs-Mache, Sen_HK
dc.date.accessioned2008-06-12T06:31:51Z-
dc.date.available2008-06-12T06:31:51Z-
dc.date.issued2003en_HK
dc.identifier.citationMolecular and Cellular Biology, 2003, v. 23 n. 9, p. 3274-3286en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49002-
dc.description.abstractAlthough it is now well documented that metazoans have evolved general transcription factor (GTF) variants to regulate their complex patterns of gene expression, there is so far no information regarding the existence of specific GTFs in plants. Here we report the characterization of a ubiquitously expressed gene that encodes a bona fide novel transcription factor IIB (TFIIB)-related protein in Arabidopsis thaliana. We have shown that this protein is the founding member of a plant-specific TFIIB-related protein family named pBrp (for plant-specific TFIIB-related protein). Surprisingly, in contrast to common GTFs that are localized in the nucleus, the bulk of pBrp proteins are bound to the cytoplasmic face of the plastid envelope, suggesting an organelle-specific function for this novel class of TFIIB-related protein. We show that pBrp proteins harbor conditional proteolytic signals that can target these proteins for rapid turnover by the proteasome-mediated protein degradation pathway. Interestingly, under conditions of proteasome inhibition, pBrp proteins accumulate in the nucleus. Together, our results suggest a possible involvement of these proteins in an intracellular signaling pathway between plastids and the nucleus. Our data provide the first evidence for an organelle-related evolution of the eukaryotic general transcription machinery.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular and Cellular Biology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Molecular and Cellular Biology, 2003, v. 23 n. 9, p. 3274-3286en_HK
dc.subject.meshArabidopsis - cytology - genetics - metabolismen_HK
dc.subject.meshArabidopsis Proteins - genetics - metabolismen_HK
dc.subject.meshTranscription Factor TFIIB - genetics - metabolismen_HK
dc.subject.meshTranscription Factors - genetics - metabolismen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleTranscription factor IIB (TFIIB)-related protein (pBrp), a plant-specific member of the TFIIB-related protein familyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-7306&volume=23&issue=9&spage=3274&epage=3286&date=2003&atitle=Transcription+factor+IIB+(TFIIB)-related+protein+(pBrp),+a+plant-specific+member+of+the+TFIIB-related+protein+familyen_HK
dc.identifier.emailLam, E: ericl89@hotmail.comen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/MCB.23.9.3274-3286.2003en_HK
dc.identifier.pmid12697827-
dc.identifier.pmcidPMC153204en_HK
dc.identifier.scopuseid_2-s2.0-0037405692-
dc.identifier.hkuros81884-
dc.identifier.isiWOS:000182325500022-

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