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Article: Proteolytic cleavage of PDZD2 generates a secreted peptide containing two PDZ domains

TitleProteolytic cleavage of PDZD2 generates a secreted peptide containing two PDZ domains
Authors
Issue Date2003
PublisherNature Publishing Group. The Journal's web site is located at http://www.emboreports.org
Citation
Embo Reports, 2003, v. 4 n. 4, p. 412-418 How to Cite?
AbstractPDZD2 (PDZ-domain-containing 2; also known as PAPIN, AIPC and PIN1) is a ubiquitously expressed multi-PDZ-domain protein. We have shown that PDZD2, which shows extensive homology to pro-interleukin-16 (pro-IL-16), is localized mainly to the endoplasmic reticulum (ER). Pro-IL-16 is cleaved in a caspase-3-dependent mechanism to generate the secreted cytokine IL-16. The abundant expression of PDZD2 in the ER, and its sequence similarity to pro-IL-16, suggests that similar post-translational processing of PDZD2 may occur. Indeed, western blotting and mass spectrometry analysis of conditioned medium from cells transfected with epitope-tagged PDZD2 show that there is secretion of a PDZD2 peptide of approximately 37 kDa (sPDZD2, for secreted PDZD2) that contains two PDZ domains. Expression of PDZD2 was detected in several tissues. Furthermore, sPDZD2 secretion is suppressed by the mutation of a sequence that shows similarity to caspase recognition motifs or by treatment with a caspase inhibitor. In summary, PDZD2 is the first reported multi-PDZ protein that is processed by proteolytic cleavage to generate a secreted peptide containing two PDZ domains.
Persistent Identifierhttp://hdl.handle.net/10722/48983
ISSN
2015 Impact Factor: 7.739
2015 SCImago Journal Rankings: 4.291
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, MLen_HK
dc.contributor.authorTam, TSMen_HK
dc.contributor.authorTsang, ACCen_HK
dc.contributor.authorYao, KMen_HK
dc.date.accessioned2008-06-12T06:31:21Z-
dc.date.available2008-06-12T06:31:21Z-
dc.date.issued2003en_HK
dc.identifier.citationEmbo Reports, 2003, v. 4 n. 4, p. 412-418en_HK
dc.identifier.issn1469-221Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/48983-
dc.description.abstractPDZD2 (PDZ-domain-containing 2; also known as PAPIN, AIPC and PIN1) is a ubiquitously expressed multi-PDZ-domain protein. We have shown that PDZD2, which shows extensive homology to pro-interleukin-16 (pro-IL-16), is localized mainly to the endoplasmic reticulum (ER). Pro-IL-16 is cleaved in a caspase-3-dependent mechanism to generate the secreted cytokine IL-16. The abundant expression of PDZD2 in the ER, and its sequence similarity to pro-IL-16, suggests that similar post-translational processing of PDZD2 may occur. Indeed, western blotting and mass spectrometry analysis of conditioned medium from cells transfected with epitope-tagged PDZD2 show that there is secretion of a PDZD2 peptide of approximately 37 kDa (sPDZD2, for secreted PDZD2) that contains two PDZ domains. Expression of PDZD2 was detected in several tissues. Furthermore, sPDZD2 secretion is suppressed by the mutation of a sequence that shows similarity to caspase recognition motifs or by treatment with a caspase inhibitor. In summary, PDZD2 is the first reported multi-PDZ protein that is processed by proteolytic cleavage to generate a secreted peptide containing two PDZ domains.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.emboreports.orgen_HK
dc.relation.ispartofEMBO Reportsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAdaptor Proteins, Signal Transducingen_HK
dc.subject.meshCarrier Proteins - chemistry - genetics - metabolismen_HK
dc.subject.meshNeoplasm Proteins - chemistry - metabolismen_HK
dc.subject.meshProstatic Neoplasms - chemistry - metabolismen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.titleProteolytic cleavage of PDZD2 generates a secreted peptide containing two PDZ domainsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1469-221X&volume=4&issue=4&spage=412&epage=418&date=2003&atitle=Proteolytic+cleavage+of+PDZD2+generates+a+secreted+peptide+containing+two+PDZ+domainsen_HK
dc.identifier.emailYeung, ML:pmlyeung@hku.hken_HK
dc.identifier.emailYao, KM:kmyao@hku.hken_HK
dc.identifier.authorityYeung, ML=rp01402en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1038/sj.embor.embor804en_HK
dc.identifier.pmid12671685-
dc.identifier.pmcidPMC1319160en_HK
dc.identifier.scopuseid_2-s2.0-0037764784en_HK
dc.identifier.hkuros84834-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037764784&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume4en_HK
dc.identifier.issue4en_HK
dc.identifier.spage412en_HK
dc.identifier.epage418en_HK
dc.identifier.isiWOS:000182801300016-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYeung, ML=8350940900en_HK
dc.identifier.scopusauthoridTam, TSM=12772669500en_HK
dc.identifier.scopusauthoridTsang, ACC=7006979260en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK

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