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- Publisher Website: 10.1074/jbc.272.32.19827
- Scopus: eid_2-s2.0-0030741972
- PMID: 9242644
- WOS: WOS:A1997XQ05900031
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Article: Molecular analysis of a novel winged helix protein, WIN: Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain
Title | Molecular analysis of a novel winged helix protein, WIN: Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain |
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Authors | |
Issue Date | 1997 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal of Biological Chemistry, 1997, v. 272 n. 32, p. 19827-19836 How to Cite? |
Abstract | We have cloned a novel winged helix factor, WIN, from the rat insulinoma cell line, INS-1. Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in thymus, testis, lung, and several intestinal regions. We determined the DNA sequences bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction- based selection procedure. WIN was found to bind with high affinity to the selected sequence 5'-AGATTGAGTA-3', which is similar to the recently identified HNF-6 binding sequence 5'-DHWATTGAYTWWD-3' (where W = A or T, Y = T or C, H is not G, and D is not C). We have isolated human WIN cDNAs by library screening and 5'-rapid amplification of cDNA ends. Sequence analysis indicates that the carboxyl terminus of human WIN has been previously isolated as a putative phosphorylation substrate, MPM2-reactive phosphoprotein 2 (MPP2); WIN may be regulated by phosphorylation. Alignment of the rat and human WIN cDNAs and their comparison with mouse genomic sequence revealed that the WIN DNA binding domain is encoded by four exons, two of which (exons 4 and 6) are alternatively spliced to generate at least three classes of mRNA transcripts. These transcripts were shown by RNase protection assay to be differentially expressed in different tissues. Alternative splicing within the winged helix DNA binding domain might result in modulation of DNA binding specificity. |
Persistent Identifier | http://hdl.handle.net/10722/48970 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yao, KM | en_HK |
dc.contributor.author | Sha, M | en_HK |
dc.contributor.author | Lu, Z | en_HK |
dc.contributor.author | Wong, GG | en_HK |
dc.date.accessioned | 2008-06-12T06:31:04Z | - |
dc.date.available | 2008-06-12T06:31:04Z | - |
dc.date.issued | 1997 | en_HK |
dc.identifier.citation | Journal of Biological Chemistry, 1997, v. 272 n. 32, p. 19827-19836 | en_HK |
dc.identifier.issn | 0021-9258 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/48970 | - |
dc.description.abstract | We have cloned a novel winged helix factor, WIN, from the rat insulinoma cell line, INS-1. Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in thymus, testis, lung, and several intestinal regions. We determined the DNA sequences bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction- based selection procedure. WIN was found to bind with high affinity to the selected sequence 5'-AGATTGAGTA-3', which is similar to the recently identified HNF-6 binding sequence 5'-DHWATTGAYTWWD-3' (where W = A or T, Y = T or C, H is not G, and D is not C). We have isolated human WIN cDNAs by library screening and 5'-rapid amplification of cDNA ends. Sequence analysis indicates that the carboxyl terminus of human WIN has been previously isolated as a putative phosphorylation substrate, MPM2-reactive phosphoprotein 2 (MPP2); WIN may be regulated by phosphorylation. Alignment of the rat and human WIN cDNAs and their comparison with mouse genomic sequence revealed that the WIN DNA binding domain is encoded by four exons, two of which (exons 4 and 6) are alternatively spliced to generate at least three classes of mRNA transcripts. These transcripts were shown by RNase protection assay to be differentially expressed in different tissues. Alternative splicing within the winged helix DNA binding domain might result in modulation of DNA binding specificity. | en_HK |
dc.format.extent | 418 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | eng | en_HK |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_HK |
dc.relation.ispartof | Journal of Biological Chemistry | en_HK |
dc.subject.mesh | Alternative Splicing | en_HK |
dc.subject.mesh | DNA - metabolism | en_HK |
dc.subject.mesh | Helix-Loop-Helix Motifs | en_HK |
dc.subject.mesh | DNA-Binding Proteins - genetics - metabolism | en_HK |
dc.subject.mesh | Transcription Factors | en_HK |
dc.title | Molecular analysis of a novel winged helix protein, WIN: Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Yao, KM:kmyao@hku.hk | en_HK |
dc.identifier.authority | Yao, KM=rp00344 | en_HK |
dc.description.nature | link_to_OA_fulltext | en_HK |
dc.identifier.doi | 10.1074/jbc.272.32.19827 | en_HK |
dc.identifier.pmid | 9242644 | - |
dc.identifier.scopus | eid_2-s2.0-0030741972 | en_HK |
dc.identifier.hkuros | 36586 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0030741972&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 272 | en_HK |
dc.identifier.issue | 32 | en_HK |
dc.identifier.spage | 19827 | en_HK |
dc.identifier.epage | 19836 | en_HK |
dc.identifier.isi | WOS:A1997XQ05900031 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Yao, KM=7403234578 | en_HK |
dc.identifier.scopusauthorid | Sha, M=7004461592 | en_HK |
dc.identifier.scopusauthorid | Lu, Z=7404769521 | en_HK |
dc.identifier.scopusauthorid | Wong, GG=7402527602 | en_HK |
dc.identifier.issnl | 0021-9258 | - |