File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Molecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain

TitleMolecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domain
Authors
Issue Date1997
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 1997, v. 272 n. 32, p. 19827-19836 How to Cite?
AbstractWe have cloned a novel winged helix factor, WIN, from the rat insulinoma cell line, INS-1. Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in thymus, testis, lung, and several intestinal regions. We determined the DNA sequences bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction- based selection procedure. WIN was found to bind with high affinity to the selected sequence 5'-AGATTGAGTA-3', which is similar to the recently identified HNF-6 binding sequence 5'-DHWATTGAYTWWD-3' (where W = A or T, Y = T or C, H is not G, and D is not C). We have isolated human WIN cDNAs by library screening and 5'-rapid amplification of cDNA ends. Sequence analysis indicates that the carboxyl terminus of human WIN has been previously isolated as a putative phosphorylation substrate, MPM2-reactive phosphoprotein 2 (MPP2); WIN may be regulated by phosphorylation. Alignment of the rat and human WIN cDNAs and their comparison with mouse genomic sequence revealed that the WIN DNA binding domain is encoded by four exons, two of which (exons 4 and 6) are alternatively spliced to generate at least three classes of mRNA transcripts. These transcripts were shown by RNase protection assay to be differentially expressed in different tissues. Alternative splicing within the winged helix DNA binding domain might result in modulation of DNA binding specificity.
Persistent Identifierhttp://hdl.handle.net/10722/48970
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, KMen_HK
dc.contributor.authorSha, Men_HK
dc.contributor.authorLu, Zen_HK
dc.contributor.authorWong, GGen_HK
dc.date.accessioned2008-06-12T06:31:04Z-
dc.date.available2008-06-12T06:31:04Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of Biological Chemistry, 1997, v. 272 n. 32, p. 19827-19836en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48970-
dc.description.abstractWe have cloned a novel winged helix factor, WIN, from the rat insulinoma cell line, INS-1. Northern blot analysis demonstrated that WIN is highly expressed in a variety of insulinoma cell lines and rat embryonic pancreas and liver. In adults, WIN expression was detected in thymus, testis, lung, and several intestinal regions. We determined the DNA sequences bound in vitro by baculovirus-expressed WIN protein in a polymerase chain reaction- based selection procedure. WIN was found to bind with high affinity to the selected sequence 5'-AGATTGAGTA-3', which is similar to the recently identified HNF-6 binding sequence 5'-DHWATTGAYTWWD-3' (where W = A or T, Y = T or C, H is not G, and D is not C). We have isolated human WIN cDNAs by library screening and 5'-rapid amplification of cDNA ends. Sequence analysis indicates that the carboxyl terminus of human WIN has been previously isolated as a putative phosphorylation substrate, MPM2-reactive phosphoprotein 2 (MPP2); WIN may be regulated by phosphorylation. Alignment of the rat and human WIN cDNAs and their comparison with mouse genomic sequence revealed that the WIN DNA binding domain is encoded by four exons, two of which (exons 4 and 6) are alternatively spliced to generate at least three classes of mRNA transcripts. These transcripts were shown by RNase protection assay to be differentially expressed in different tissues. Alternative splicing within the winged helix DNA binding domain might result in modulation of DNA binding specificity.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAlternative Splicingen_HK
dc.subject.meshDNA - metabolismen_HK
dc.subject.meshHelix-Loop-Helix Motifsen_HK
dc.subject.meshDNA-Binding Proteins - genetics - metabolismen_HK
dc.subject.meshTranscription Factorsen_HK
dc.titleMolecular analysis of a novel winged helix protein, WIN. Expression pattern, DNA binding property, and alternative splicing within the DNA binding domainen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=272&issue=32&spage=19827&epage=19836&date=1997&atitle=Molecular+analysis+of+a+novel+winged+helix+protein,+WIN:+expression+pattern,+DNA+binding+property+and+alternative+splicing+within+the+DNA+binding+domainen_HK
dc.identifier.emailYao, KM:kmyao@hku.hken_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1074/jbc.272.32.19827en_HK
dc.identifier.pmid9242644-
dc.identifier.scopuseid_2-s2.0-0030741972en_HK
dc.identifier.hkuros36586-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030741972&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume272en_HK
dc.identifier.issue32en_HK
dc.identifier.spage19827en_HK
dc.identifier.epage19836en_HK
dc.identifier.isiWOS:A1997XQ05900031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridSha, M=7004461592en_HK
dc.identifier.scopusauthoridLu, Z=7404769521en_HK
dc.identifier.scopusauthoridWong, GG=7402527602en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats