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Article: Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

TitleMale accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters
Authors
KeywordsCa2+ oscillation
Golden hamster
In-vitro fertilization
Male accessory sex glands
Oocyte
Issue Date1999
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 1999, v. 5 n. 6, p. 527-533 How to Cite?
AbstractTo evaluate the effect of male accessory sex gland secretions on Ca2+ oscillations of oocytes, epididymal or ejaculated spermatozoa recovered from uteri were used to inseminate oocytes. Ca2+ oscillations were measured by Fura 2 fluorescence imaging (F340/F380). We showed that although Ca2+ oscillations induced by ejaculated spermatozoa had a pattern similar to those induced by epididymal spermatozoa, the amplitude of the first Ca2+ transient in the former group was significantly higher (P < 0.05) and the duration was significantly longer (P < 0.01). Oocytes inseminated with ejaculated spermatozoa recovered from uteri from males had ampullary glands or ventral prostates removed showed significantly lower Ca2+ oscillations compared to the controls (P < 0.05, P < 0.01 respectively). Moreover, the relative area of the first Ca2+ transient in treatment groups was significantly smaller than the control. In addition, a significantly higher percentage of oocytes (52%) inseminated by spermatozoa from males with all accessory sex glands removed showed non-oscillatory Ca2+ transients, compared to the controls (5%, P < 0.05). These results indicate that accessory sex gland secretions can affect Ca2+ oscillations. The differences between Ca2+ oscillations induced by epididymal and uterine spermatozoa from males with all accessory sex glands removed suggest that uterine factors may also influence this process.
Persistent Identifierhttp://hdl.handle.net/10722/48955
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYing, Yen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorCheung, MPLen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2008-06-12T06:30:38Z-
dc.date.available2008-06-12T06:30:38Z-
dc.date.issued1999en_HK
dc.identifier.citationMolecular Human Reproduction, 1999, v. 5 n. 6, p. 527-533en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48955-
dc.description.abstractTo evaluate the effect of male accessory sex gland secretions on Ca2+ oscillations of oocytes, epididymal or ejaculated spermatozoa recovered from uteri were used to inseminate oocytes. Ca2+ oscillations were measured by Fura 2 fluorescence imaging (F340/F380). We showed that although Ca2+ oscillations induced by ejaculated spermatozoa had a pattern similar to those induced by epididymal spermatozoa, the amplitude of the first Ca2+ transient in the former group was significantly higher (P < 0.05) and the duration was significantly longer (P < 0.01). Oocytes inseminated with ejaculated spermatozoa recovered from uteri from males had ampullary glands or ventral prostates removed showed significantly lower Ca2+ oscillations compared to the controls (P < 0.05, P < 0.01 respectively). Moreover, the relative area of the first Ca2+ transient in treatment groups was significantly smaller than the control. In addition, a significantly higher percentage of oocytes (52%) inseminated by spermatozoa from males with all accessory sex glands removed showed non-oscillatory Ca2+ transients, compared to the controls (5%, P < 0.05). These results indicate that accessory sex gland secretions can affect Ca2+ oscillations. The differences between Ca2+ oscillations induced by epididymal and uterine spermatozoa from males with all accessory sex glands removed suggest that uterine factors may also influence this process.en_HK
dc.format.extent422 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCa2+ oscillationen_HK
dc.subjectGolden hamsteren_HK
dc.subjectIn-vitro fertilizationen_HK
dc.subjectMale accessory sex glandsen_HK
dc.subjectOocyteen_HK
dc.titleMale accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamstersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1360-9947&volume=5&issue=6&spage=527&epage=533&date=1999&atitle=Male+accessory+sex+gland+secretions+affect+oocyte+Ca2++oscillations+during+in-vitro+fertilization+in+gold+hamstersen_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/molehr/5.6.527en_HK
dc.identifier.pmid10340999en_HK
dc.identifier.scopuseid_2-s2.0-0033044967en_HK
dc.identifier.hkuros40877-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033044967&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue6en_HK
dc.identifier.spage527en_HK
dc.identifier.epage533en_HK
dc.identifier.isiWOS:000080661600006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYing, Y=36889049700en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridCheung, MPL=16749051200en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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