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Article: Effects of male accessory sex glands on deoxyribonucleic acid synthesis in the first cell cycle of golden hamster embryos

TitleEffects of male accessory sex glands on deoxyribonucleic acid synthesis in the first cell cycle of golden hamster embryos
Authors
Issue Date1998
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 1998, v. 58 n. 3, p. 659-663 How to Cite?
AbstractTo study the paternal effect, particularly of accessory sex gland secretions, on DNA synthesis in golden hamster zygotes, the glands were surgically removed from golden hamsters resulting in 4 groups: SH, sham- operated; AGX, bilateral excision of ampullary glands; VPX, bilateral excision of ventral prostates; and TX, excision of all accessory sex glands. Each female was mated with one male and killed at 6, 8, 10, or 12 h postcoitus (p.c.). Embryos were collected, and zygotes in S-phase were identified by immunocytochemical techniques after pulse-labeling with 50 μM 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU) in Tyrode's albumin lactate pyruvate medium for 30 min. In another experiment, embryos at 6 h p.c. were incubated in 50 μM BrdU for 6 h. Presumptive zygotes were stained with propidium iodide to visualize pronuclei. The BrdU pulse-labeling results showed that the percentages of embryos with 1 or 2 pronuclei in S-phase at 8 h p.c. in VPX (44.3 ± 10.4) and TX (41.6 ± 10.6) groups were significantly lower (p < 0.05) than that in the SH group (69.0 ± 5.5). In the AGX group, although the percentage of embryos with I or 2 pronuclei in S-phase was not significantly different from that of the SH group, the percentage of 8-h embryos with 2 pronuclei in S-phase was 51.6 ± 2.8, significantly lower than in the SH control (68.1 ± 6.0, p < 0.05). When the embryos were labeled with BrdU for 6 h, the percentages of embryos with 1 or 2 pronuclei in S-phase were not different among the 4 groups. However, the percentage of embryos showing 2 pronuclei in S-phase was significantly lower in the AGX group (78.4 ± 6.7) than in the SH group (91.8 ± 2.9, p < 0.05). These results suggest that accessory sex glands can affect DNA synthesis in hamster zygotes and that the mechanisms by which ampullary gland and ventral prostrate secretions affect the first cell cycle are probably different. A significantly higher incidence (p < 0.001) of polyspermy was observed in embryos sired by males without ampullary glands (5.2 ± 1.0%) compared with those sired by the SH group (0.8 ± 0.3%).
Persistent Identifierhttp://hdl.handle.net/10722/48952
ISSN
2015 Impact Factor: 3.471
2015 SCImago Journal Rankings: 1.646
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYing, Yen_HK
dc.contributor.authorChow, PHen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2008-06-12T06:30:34Z-
dc.date.available2008-06-12T06:30:34Z-
dc.date.issued1998en_HK
dc.identifier.citationBiology Of Reproduction, 1998, v. 58 n. 3, p. 659-663en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48952-
dc.description.abstractTo study the paternal effect, particularly of accessory sex gland secretions, on DNA synthesis in golden hamster zygotes, the glands were surgically removed from golden hamsters resulting in 4 groups: SH, sham- operated; AGX, bilateral excision of ampullary glands; VPX, bilateral excision of ventral prostates; and TX, excision of all accessory sex glands. Each female was mated with one male and killed at 6, 8, 10, or 12 h postcoitus (p.c.). Embryos were collected, and zygotes in S-phase were identified by immunocytochemical techniques after pulse-labeling with 50 μM 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU) in Tyrode's albumin lactate pyruvate medium for 30 min. In another experiment, embryos at 6 h p.c. were incubated in 50 μM BrdU for 6 h. Presumptive zygotes were stained with propidium iodide to visualize pronuclei. The BrdU pulse-labeling results showed that the percentages of embryos with 1 or 2 pronuclei in S-phase at 8 h p.c. in VPX (44.3 ± 10.4) and TX (41.6 ± 10.6) groups were significantly lower (p < 0.05) than that in the SH group (69.0 ± 5.5). In the AGX group, although the percentage of embryos with I or 2 pronuclei in S-phase was not significantly different from that of the SH group, the percentage of 8-h embryos with 2 pronuclei in S-phase was 51.6 ± 2.8, significantly lower than in the SH control (68.1 ± 6.0, p < 0.05). When the embryos were labeled with BrdU for 6 h, the percentages of embryos with 1 or 2 pronuclei in S-phase were not different among the 4 groups. However, the percentage of embryos showing 2 pronuclei in S-phase was significantly lower in the AGX group (78.4 ± 6.7) than in the SH group (91.8 ± 2.9, p < 0.05). These results suggest that accessory sex glands can affect DNA synthesis in hamster zygotes and that the mechanisms by which ampullary gland and ventral prostrate secretions affect the first cell cycle are probably different. A significantly higher incidence (p < 0.001) of polyspermy was observed in embryos sired by males without ampullary glands (5.2 ± 1.0%) compared with those sired by the SH group (0.8 ± 0.3%).en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshDNA - biosynthesisen_HK
dc.subject.meshGenitalia, Male - physiologyen_HK
dc.subject.meshZygote - cytology - metabolismen_HK
dc.subject.meshBromodeoxyuridine - metabolismen_HK
dc.subject.meshCell Cycle - physiologyen_HK
dc.titleEffects of male accessory sex glands on deoxyribonucleic acid synthesis in the first cell cycle of golden hamster embryosen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=58&issue=3&spage=659&epage=663&date=1998&atitle=Effects+of+male+accessory+sex+glands+on+deoxyribonucleic+acid+synthesis+in+the+first+cell+cycle+of+golden+hamster+embryosen_HK
dc.identifier.emailOWS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityOWS=rp00315en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1095/biolreprod58.3.659en_HK
dc.identifier.pmid9510953-
dc.identifier.scopuseid_2-s2.0-0031933236en_HK
dc.identifier.hkuros30144-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031933236&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume58en_HK
dc.identifier.issue3en_HK
dc.identifier.spage659en_HK
dc.identifier.epage663en_HK
dc.identifier.isiWOS:000072193100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYing, Y=36889049700en_HK
dc.identifier.scopusauthoridChow, PH=7202656919en_HK
dc.identifier.scopusauthoridOWS=6701729369en_HK

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