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Article: The influence of predegenerated nerve grafts on axonal regeneration from prelesioned peripheral nerves

TitleThe influence of predegenerated nerve grafts on axonal regeneration from prelesioned peripheral nerves
Authors
KeywordsPeripheral nerve regeneration
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOA
Citation
Journal Of Anatomy, 1996, v. 189 n. 2, p. 293-302 How to Cite?
AbstractRecent in vitro work has indicated that predegenerated segments of peripheral nerve are more capable of supporting neurite growth from adult neurons than fresh segments of nerve, whereas previous in vivo studies which investigated whether predegenerated nerve segments used as grafts are capable of enhancing axonal regeneration produced conflicting results. We have reinvestigated this question by using predegenerated nerve grafts in combination with conditioning lesions of the host nerve to determine the optimal conditions for obtaining the maximal degree of regeneration of myelinated axons. The sciatic nerve of adult Dark Agouti rats were sectioned at midthigh level, and the distal portion was allowed to predegenerate for 0, 6 or 12 d in situ. 10-15 mm lengths of these distal nerve segments were then syngenically grafted onto the central stumps of sciatic nerves which had themselves received a conditioning lesion 0, 6, and 12 d previously, making a total of 9 different donor-host combinations. The grafts were assessed histologically 3 or 8 wk after grafting. Axonal regeneration in the 9 different donor-host combinations was determined by counting the numbers of myelinated axons in transverse sections through the grafts. All grafts examined contained regenerating myelinated axons. The rats given a 3 wk postgrafting survival period had an average of between 1400 and 5300 such axons. The rats given an 8 wk postgrafting survival period had between about 13 000 and 25 000 regenerating myelinated axons. Analysis of variance revealed significant main effects for both the Donor and Host conditions as well as Weeks (i.e. survival period after grafting). These results indicate that both a conditioning lesion of the host neurons and the degree of predegeneration of peripheral nerve segments to be used as grafts are of importance in influencing the degree of axonal regeneration. Of these 2 factors the conditioning lesion of the host appears to have the greater effect on the final number of regenerating myelinated axons.
Persistent Identifierhttp://hdl.handle.net/10722/48950
ISSN
2015 Impact Factor: 2.154
2015 SCImago Journal Rankings: 1.056
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHasan, NAKESen_HK
dc.contributor.authorNeumann, MMen_HK
dc.contributor.authorDe Souky, MAen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorBedi, KSen_HK
dc.date.accessioned2008-06-12T06:30:31Z-
dc.date.available2008-06-12T06:30:31Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal Of Anatomy, 1996, v. 189 n. 2, p. 293-302en_HK
dc.identifier.issn0021-8782en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48950-
dc.description.abstractRecent in vitro work has indicated that predegenerated segments of peripheral nerve are more capable of supporting neurite growth from adult neurons than fresh segments of nerve, whereas previous in vivo studies which investigated whether predegenerated nerve segments used as grafts are capable of enhancing axonal regeneration produced conflicting results. We have reinvestigated this question by using predegenerated nerve grafts in combination with conditioning lesions of the host nerve to determine the optimal conditions for obtaining the maximal degree of regeneration of myelinated axons. The sciatic nerve of adult Dark Agouti rats were sectioned at midthigh level, and the distal portion was allowed to predegenerate for 0, 6 or 12 d in situ. 10-15 mm lengths of these distal nerve segments were then syngenically grafted onto the central stumps of sciatic nerves which had themselves received a conditioning lesion 0, 6, and 12 d previously, making a total of 9 different donor-host combinations. The grafts were assessed histologically 3 or 8 wk after grafting. Axonal regeneration in the 9 different donor-host combinations was determined by counting the numbers of myelinated axons in transverse sections through the grafts. All grafts examined contained regenerating myelinated axons. The rats given a 3 wk postgrafting survival period had an average of between 1400 and 5300 such axons. The rats given an 8 wk postgrafting survival period had between about 13 000 and 25 000 regenerating myelinated axons. Analysis of variance revealed significant main effects for both the Donor and Host conditions as well as Weeks (i.e. survival period after grafting). These results indicate that both a conditioning lesion of the host neurons and the degree of predegeneration of peripheral nerve segments to be used as grafts are of importance in influencing the degree of axonal regeneration. Of these 2 factors the conditioning lesion of the host appears to have the greater effect on the final number of regenerating myelinated axons.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOAen_HK
dc.relation.ispartofJournal of Anatomyen_HK
dc.rightsJournal of Anatomy: molecular, cellular and experimental morphology. Copyright © Blackwell Publishing Ltd.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_HK
dc.subjectPeripheral nerve regenerationen_HK
dc.titleThe influence of predegenerated nerve grafts on axonal regeneration from prelesioned peripheral nervesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8782&volume=189&issue=Pt 2&spage=293&epage=302&date=1996&atitle=The+influence+of+predegenerated+nerve+grafts+on+axonal+regeneration+from+prelesioned+peripheral+nervesen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8886951-
dc.identifier.pmcidPMC1167746-
dc.identifier.scopuseid_2-s2.0-0029908715en_HK
dc.identifier.hkuros21776-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029908715&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume189en_HK
dc.identifier.issue2en_HK
dc.identifier.spage293en_HK
dc.identifier.epage302en_HK
dc.identifier.isiWOS:A1996VP26400005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHasan, NAKES=16935204700en_HK
dc.identifier.scopusauthoridNeumann, MM=16936238800en_HK
dc.identifier.scopusauthoridDe Souky, MA=16934333900en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridBedi, KS=7005465634en_HK

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