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Article: A quantitative study of silver-stained NORs in different segments of the normal human colorectal crypt

TitleA quantitative study of silver-stained NORs in different segments of the normal human colorectal crypt
Authors
KeywordsAgNORs
Colorectum
Stereology
Epithelium
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOA
Citation
Journal of Anatomy: molecular, cellular and experimental morphology, 1996, v. 188 n. Pt 3, p. 521-527 How to Cite?
AbstractQuantification of silver-stained nucleolar organiser regions (AgNORs) in paraffin sections may provide clues about the proliferation and differentiation in normal and neoplastic tissues. The aim of the present investigation was to determine whether AgNOR quantification could provide useful data about proliferation in the different segments of the normal human colorectal crypt. Samples of histologically 'normal' large intestine (n = 8) were obtained from colorectal cancer resections at a distance of > 5 cm from the tumour margins and were routinely processed for paraffin embedding using strictly standardised procedures. Sections were cut and stained with a one-stage silver colloid impregnation technique. The longitudinally sectioned crypts were divided into proliferative (P), intermediate (I) and surface (S) segments using strict criteria. Clearly defined AgNORs, which appeared as black dots within the nuclear profile, were quantified from each segment for volume density (Vv) and number per unit area (NA) estimates using traditional point-counting techniques. A 1-way analysis of variance followed by Scheffe's test indicated significant progressive reductions of AgNOR Vv and NA from P to S segments. Our data suggest that both volume and frequency of AgNORs may be related to cellular proliferation since both parameters are highest in the P segment. The further exploitation of stereological tools in conjunction with AgNOR staining may be valuable in assessing normal differentiation and proliferation patterns and in predicting the biological behaviour of neoplastic tissues in which increased proliferation is a feature.
Persistent Identifierhttp://hdl.handle.net/10722/48949
ISSN
2015 Impact Factor: 2.154
2015 SCImago Journal Rankings: 1.056
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMorais, Men_HK
dc.contributor.authorDockery, Pen_HK
dc.contributor.authorWhite, FHen_HK
dc.date.accessioned2008-06-12T06:30:29Z-
dc.date.available2008-06-12T06:30:29Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal of Anatomy: molecular, cellular and experimental morphology, 1996, v. 188 n. Pt 3, p. 521-527en_HK
dc.identifier.issn0021-8782en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48949-
dc.description.abstractQuantification of silver-stained nucleolar organiser regions (AgNORs) in paraffin sections may provide clues about the proliferation and differentiation in normal and neoplastic tissues. The aim of the present investigation was to determine whether AgNOR quantification could provide useful data about proliferation in the different segments of the normal human colorectal crypt. Samples of histologically 'normal' large intestine (n = 8) were obtained from colorectal cancer resections at a distance of > 5 cm from the tumour margins and were routinely processed for paraffin embedding using strictly standardised procedures. Sections were cut and stained with a one-stage silver colloid impregnation technique. The longitudinally sectioned crypts were divided into proliferative (P), intermediate (I) and surface (S) segments using strict criteria. Clearly defined AgNORs, which appeared as black dots within the nuclear profile, were quantified from each segment for volume density (Vv) and number per unit area (NA) estimates using traditional point-counting techniques. A 1-way analysis of variance followed by Scheffe's test indicated significant progressive reductions of AgNOR Vv and NA from P to S segments. Our data suggest that both volume and frequency of AgNORs may be related to cellular proliferation since both parameters are highest in the P segment. The further exploitation of stereological tools in conjunction with AgNOR staining may be valuable in assessing normal differentiation and proliferation patterns and in predicting the biological behaviour of neoplastic tissues in which increased proliferation is a feature.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOAen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Anatomy: molecular, cellular and experimental morphology. Copyright © Blackwell Publishing Ltd.en_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_HK
dc.subjectAgNORsen_HK
dc.subjectColorectumen_HK
dc.subjectStereologyen_HK
dc.subjectEpitheliumen_HK
dc.titleA quantitative study of silver-stained NORs in different segments of the normal human colorectal crypten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8782&volume=188&issue=Pt 3&spage=521&epage=527&date=1996&atitle=A+quantitative+study+of+silver-stained+NORs+in+different+segments+of+the+normal+human+colorectal+crypten_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8763469-
dc.identifier.pmcidPMC1167480-
dc.identifier.scopuseid_2-s2.0-0030036106-
dc.identifier.hkuros20177-
dc.identifier.isiWOS:A1996UX32200002-

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