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Article: The human ovarian granulosa cell: a stereological approach

TitleThe human ovarian granulosa cell: a stereological approach
Authors
KeywordsOvary
Stereology
Selector
In vitro fertilisation
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOA
Citation
Journal of Anatomy: molecular, cellular and experimental morphology, 1996, v. 188 n. Pt 3, p. 671-676 How to Cite?
AbstractThe human ovarian granulosa cell is perhaps the most widely studied endocrine cell, but little quantitative structural information exists for this cell. In the present study new and traditional stereological probes have been employed to provide quantitative structural information on these functionally important cells. Granulosa cells were obtained from follicular aspirations from 10 women during in vitro fertilisation procedures. Initially 2 methods were used to estimate the mean nuclear volume of these cells: the mean number weighted nuclear volume was estimated by the Selector and the mean volume weighted nuclear volume by the point sampled intercept method. It was found that the difference between the 2 volume estimates was only 8.5%. The volume weighted mean nuclear volume was used as an estimate of nuclear volume. This was subsequently corrected (taking the percentage difference as the empirical bias) and combined with fractional cell volumes (Vv) to produce estimates of cell, mitochondrial, lipid and nucleolar volume. The proportion of the cell occupied by the nucleus had a remarkably low interindividual variation (CV = 7.6%). The proportion of the nucleus occupied by euchromatin also had a striking low variation (CV < 6%). All other cellular parameters had CVs of less than 35%. The lipid composition of these cells showed the greatest interindividual variability, with a CV of 42% for relative and 54% for absolute volume. The present study outlines a simple protocol for the quantitation of granulosa cell structure using new unbiased stereological probes and providing baseline structural information.
Persistent Identifierhttp://hdl.handle.net/10722/48948
ISSN
2015 Impact Factor: 2.154
2015 SCImago Journal Rankings: 1.056
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDhar, Aen_HK
dc.contributor.authorDockery, Pen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorTurner, Ken_HK
dc.contributor.authorLenton, EAen_HK
dc.contributor.authorCooke, IDen_HK
dc.date.accessioned2008-06-12T06:30:28Z-
dc.date.available2008-06-12T06:30:28Z-
dc.date.issued1996en_HK
dc.identifier.citationJournal of Anatomy: molecular, cellular and experimental morphology, 1996, v. 188 n. Pt 3, p. 671-676en_HK
dc.identifier.issn0021-8782en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48948-
dc.description.abstractThe human ovarian granulosa cell is perhaps the most widely studied endocrine cell, but little quantitative structural information exists for this cell. In the present study new and traditional stereological probes have been employed to provide quantitative structural information on these functionally important cells. Granulosa cells were obtained from follicular aspirations from 10 women during in vitro fertilisation procedures. Initially 2 methods were used to estimate the mean nuclear volume of these cells: the mean number weighted nuclear volume was estimated by the Selector and the mean volume weighted nuclear volume by the point sampled intercept method. It was found that the difference between the 2 volume estimates was only 8.5%. The volume weighted mean nuclear volume was used as an estimate of nuclear volume. This was subsequently corrected (taking the percentage difference as the empirical bias) and combined with fractional cell volumes (Vv) to produce estimates of cell, mitochondrial, lipid and nucleolar volume. The proportion of the cell occupied by the nucleus had a remarkably low interindividual variation (CV = 7.6%). The proportion of the nucleus occupied by euchromatin also had a striking low variation (CV < 6%). All other cellular parameters had CVs of less than 35%. The lipid composition of these cells showed the greatest interindividual variability, with a CV of 42% for relative and 54% for absolute volume. The present study outlines a simple protocol for the quantitation of granulosa cell structure using new unbiased stereological probes and providing baseline structural information.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOAen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Anatomy: molecular, cellular and experimental morphology. Copyright © Blackwell Publishing Ltd.en_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_HK
dc.subjectOvaryen_HK
dc.subjectStereologyen_HK
dc.subjectSelectoren_HK
dc.subjectIn vitro fertilisationen_HK
dc.titleThe human ovarian granulosa cell: a stereological approachen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8782&volume=188&issue=Pt 3&spage=671&epage=676&date=1996&atitle=The+human+ovarian+granulosa+cell:+a+stereological+approachen_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8763484-
dc.identifier.pmcidPMC1167495-
dc.identifier.scopuseid_2-s2.0-0029815218-
dc.identifier.hkuros13058-
dc.identifier.isiWOS:A1996UX32200017-

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