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Article: In vitro method to study antifungal perfusion in Candida Biofilms

TitleIn vitro method to study antifungal perfusion in Candida Biofilms
Authors
Issue Date2005
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2005, v. 43 n. 2, p. 818-825 How to Cite?
AbstractAntimycotic perfusion through Candida biofilms was demonstrated by a modification of a simple in vitro diffusion cell bioassay system. Using this model, the perfusion of three commonly used antifungal agents, amphotericin B, fluconazole, and flucytosine, was investigated in biofilms of three different Candida species (i.e., Candida albicans, Candida parapsilosis, and Candida krusei) that were developed on microporous filters. Scanning electron microscopy revealed that C. albicans formed a contiguous biofilm with tightly packed blastospores and occasional hyphae compared with C. parapsilosis and C. krusei, which developed confluent biofilms displaying structural heterogeneity and a lesser cell density, after 48 h of incubation on nutrient agar. Minor structural changes were also perceptible on the superficial layers of the biofilm after antifungal perfusion. The transport of antifungals to the distal biofilm-substratum interface was most impeded by C. albicans biofilms in comparison to C. parapsilosis and C. krusei. Fluconazole and flucytosine demonstrated similar levels of perfusion, while amphotericin B was the least penetrant through all three biofilms, although the latter appeared to cause the most structural damage to the superficial cells of the biofilm compared with the other antifungals. These results suggest that the antifungal perfusion through biofilm mode of growth in Candida is dependent both on the antimycotic and the Candida species in question, and in clinical terms, these phenomena could contribute to the failure of Candida biofilm-associated infections. Finally, the in vitro model we have described should serve as a useful system to investigate the complex interactions that appear to operate in vivo within the biofilm-antifungal interphase.
Persistent Identifierhttp://hdl.handle.net/10722/48936
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorYe, Jen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2008-06-12T06:30:05Z-
dc.date.available2008-06-12T06:30:05Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2005, v. 43 n. 2, p. 818-825en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48936-
dc.description.abstractAntimycotic perfusion through Candida biofilms was demonstrated by a modification of a simple in vitro diffusion cell bioassay system. Using this model, the perfusion of three commonly used antifungal agents, amphotericin B, fluconazole, and flucytosine, was investigated in biofilms of three different Candida species (i.e., Candida albicans, Candida parapsilosis, and Candida krusei) that were developed on microporous filters. Scanning electron microscopy revealed that C. albicans formed a contiguous biofilm with tightly packed blastospores and occasional hyphae compared with C. parapsilosis and C. krusei, which developed confluent biofilms displaying structural heterogeneity and a lesser cell density, after 48 h of incubation on nutrient agar. Minor structural changes were also perceptible on the superficial layers of the biofilm after antifungal perfusion. The transport of antifungals to the distal biofilm-substratum interface was most impeded by C. albicans biofilms in comparison to C. parapsilosis and C. krusei. Fluconazole and flucytosine demonstrated similar levels of perfusion, while amphotericin B was the least penetrant through all three biofilms, although the latter appeared to cause the most structural damage to the superficial cells of the biofilm compared with the other antifungals. These results suggest that the antifungal perfusion through biofilm mode of growth in Candida is dependent both on the antimycotic and the Candida species in question, and in clinical terms, these phenomena could contribute to the failure of Candida biofilm-associated infections. Finally, the in vitro model we have described should serve as a useful system to investigate the complex interactions that appear to operate in vivo within the biofilm-antifungal interphase.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2005, v. 43 n. 2, p. 818-825en_HK
dc.subject.meshAntifungal Agents - metabolism - pharmacologyen_HK
dc.subject.meshBiofilms - drug effects - growth & developmenten_HK
dc.subject.meshCandida - classification - drug effects - growth & development - ultrastructureen_HK
dc.subject.meshAmphotericin B - metabolism - pharmacologyen_HK
dc.subject.meshBiological Assayen_HK
dc.titleIn vitro method to study antifungal perfusion in Candida Biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=43&issue=2&spage=818&epage=825&date=2005&atitle=In+vitro+method+to+study+antifungal+perfusion+in+Candida+biofilmsen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.43.2.818-825.2005en_HK
dc.identifier.pmid15695686-
dc.identifier.pmcidPMC548120en_HK
dc.identifier.scopuseid_2-s2.0-13844280924en_HK
dc.identifier.hkuros97335-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13844280924&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue2en_HK
dc.identifier.spage818en_HK
dc.identifier.epage825en_HK
dc.identifier.isiWOS:000227045600046-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridYe, J=23669624100en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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