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Article: Antifungal effects of lysozyme and lactoferrin against genetically similar, sequential candida albicans isolates from a human immunodeficiency virus-infected southern chinese cohort

TitleAntifungal effects of lysozyme and lactoferrin against genetically similar, sequential candida albicans isolates from a human immunodeficiency virus-infected southern chinese cohort
Authors
Issue Date2001
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2001, v. 39 n. 9, p. 3296-3302 How to Cite?
AbstractA variety of innate defense factors in saliva such as lysozyme and lactoferrin contribute to mucosal protection and modulate Candida populations in the oral cavity. It is also known that in human immunodeficiency virus (HIV)-infected individuals significant variations in the concentrations of lysozyme and lactoferrin in saliva occur during disease progression. Therefore, the aim of this study was to determine the in vitro susceptibility to human lactoferrin and hen egg white lysozyme of genotypically similar oral Candida albicans isolates obtained from six HIV-infected ethnic Chinese during sequential visits over a 12-month period. The similarity of the genotypes (50 in total) was evaluated using a randomly amplified polymorphic DNA assay. A blastospore viability assay was performed to evaluate the sensitivity of the organisms to lysozyme and lactoferrin. Exposure to physiological concentrations of either lysozyme (30 μg/ml) or lactoferrin (20 μg/ml) caused a rapid loss of viability among all isolates to a varying extent. None of the sequential C. albicans isolates demonstrated significant differences in sensitivity to either protein from one visit to the next; similar results were noted when the different genotypes from the same individual were compared. On Spearman correlation analysis of two genotypes that were sequentially isolated from a single patient, a significant negative correlation between lysozyme (r = -0.88; P < 0.02) (but not lactoferrin) resistance and the duration of HIV disease was seen. These results imply that a minority of C. albicans isolates that persist intraorally in individuals with HIV disease develop progressive resistance to innate salivary antifungal defenses such as lysozyme, possibly as an adaptive response. However, the vast majority of the Candida isolates appear to succumb to these nonspecific host immune mediators abundantly present in the oral environment.
Persistent Identifierhttp://hdl.handle.net/10722/48933
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorPow, EHNen_HK
dc.contributor.authorBeena, VTen_HK
dc.contributor.authorYeung, KWSen_HK
dc.date.accessioned2008-06-12T06:30:01Z-
dc.date.available2008-06-12T06:30:01Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2001, v. 39 n. 9, p. 3296-3302en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48933-
dc.description.abstractA variety of innate defense factors in saliva such as lysozyme and lactoferrin contribute to mucosal protection and modulate Candida populations in the oral cavity. It is also known that in human immunodeficiency virus (HIV)-infected individuals significant variations in the concentrations of lysozyme and lactoferrin in saliva occur during disease progression. Therefore, the aim of this study was to determine the in vitro susceptibility to human lactoferrin and hen egg white lysozyme of genotypically similar oral Candida albicans isolates obtained from six HIV-infected ethnic Chinese during sequential visits over a 12-month period. The similarity of the genotypes (50 in total) was evaluated using a randomly amplified polymorphic DNA assay. A blastospore viability assay was performed to evaluate the sensitivity of the organisms to lysozyme and lactoferrin. Exposure to physiological concentrations of either lysozyme (30 μg/ml) or lactoferrin (20 μg/ml) caused a rapid loss of viability among all isolates to a varying extent. None of the sequential C. albicans isolates demonstrated significant differences in sensitivity to either protein from one visit to the next; similar results were noted when the different genotypes from the same individual were compared. On Spearman correlation analysis of two genotypes that were sequentially isolated from a single patient, a significant negative correlation between lysozyme (r = -0.88; P < 0.02) (but not lactoferrin) resistance and the duration of HIV disease was seen. These results imply that a minority of C. albicans isolates that persist intraorally in individuals with HIV disease develop progressive resistance to innate salivary antifungal defenses such as lysozyme, possibly as an adaptive response. However, the vast majority of the Candida isolates appear to succumb to these nonspecific host immune mediators abundantly present in the oral environment.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2001, v. 39 n. 9, p. 3296-3302en_HK
dc.subject.meshAIDS-Related Opportunistic Infections - microbiologyen_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshCandida albicans - classification - drug effects - genetics - isolation & purificationen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshLactoferrin - pharmacologyen_HK
dc.titleAntifungal effects of lysozyme and lactoferrin against genetically similar, sequential candida albicans isolates from a human immunodeficiency virus-infected southern chinese cohorten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=39&issue=9&spage=3296&epage=3302&date=2001&atitle=Antifungal+effects+of+lysozyme+and+lactoferrin+against+genetically+similar,+sequential+Candida+albicans+isolates+from+a+human+immunodeficiency+virus-infected+southern+Chinese+cohorten_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailPow, EHN:ehnpow@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityPow, EHN=rp00030en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.39.9.3296-3302.2001en_HK
dc.identifier.pmid11526166-
dc.identifier.pmcidPMC88334en_HK
dc.identifier.scopuseid_2-s2.0-0034827946en_HK
dc.identifier.hkuros62642-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034827946&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3296en_HK
dc.identifier.epage3302en_HK
dc.identifier.isiWOS:000170837500044-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridPow, EHN=6603825799en_HK
dc.identifier.scopusauthoridBeena, VT=6603720754en_HK
dc.identifier.scopusauthoridYeung, KWS=13304249300en_HK

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