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Article: Cytopathic effects of Treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cells

TitleCytopathic effects of Treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cells
Authors
Issue Date1995
PublisherAmerican Society for Microbiology.
Citation
Infection And Immunity, 1995, v. 63 n. 9, p. 3401-3410 How to Cite?
AbstractThe effects of Treponema denticola and its outer membrane-bound chymotrypsin-like proteinase on periodontal ligament epithelial cell cultures at different stages of maturity were studied. In sparse cultures with migrating epithelial cells, large intracellular vacuoles were formed rapidly following exposure to live T. denticola. Treponemes showing structural damage were seen occasionally inside membrane-bound vesicles. Intensive membrane blebbing occurred in infected cells and continued for up to 48 h before the cell died. Blebbing could also be induced by a purified chymotrypsin-like proteinase of T. denticola. Cortical actin and α-actinin of the bacterium- treated cells showed disorganization, and pericellular fibronectin was degraded by both whole T. denticola and the isolated proteinase. Epithelial cells with well-formed lateral cell contacts appeared to be more resistant to the effects of T. denticola than migrating isolated cells. In multilayer epithelial cultures, adhesion of T. denticola and membrane blebbing were observed infrequently. There was no evidence of invasion of T. denticola into epithelial multilayers. However, immunogold electron microscopy showed rapid transport of T. denticola chymotrypsin-like proteinase into newly formed large intracellular vacuoles within the epithelial layers. These vacuoles were lined by membranes studded with ribosomes. T. denticola-treated epithelial multilayers had loose cell contacts, collapsed intercellular spaces, and increased permeability. Through its capacity to cause these unique cytopathic effects, the chymotrypsin-like proteinase of T. denticola has the potential to contribute to the initiation of periodontal disease.
Persistent Identifierhttp://hdl.handle.net/10722/48918
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorUitto, VJen_HK
dc.contributor.authorPan, YMen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorLarjava, Hen_HK
dc.contributor.authorEllen, RPen_HK
dc.contributor.authorFinlay, BBen_HK
dc.contributor.authorMcBride, BCen_HK
dc.date.accessioned2008-06-12T06:29:40Z-
dc.date.available2008-06-12T06:29:40Z-
dc.date.issued1995en_HK
dc.identifier.citationInfection And Immunity, 1995, v. 63 n. 9, p. 3401-3410en_HK
dc.identifier.issn0019-9567en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48918-
dc.description.abstractThe effects of Treponema denticola and its outer membrane-bound chymotrypsin-like proteinase on periodontal ligament epithelial cell cultures at different stages of maturity were studied. In sparse cultures with migrating epithelial cells, large intracellular vacuoles were formed rapidly following exposure to live T. denticola. Treponemes showing structural damage were seen occasionally inside membrane-bound vesicles. Intensive membrane blebbing occurred in infected cells and continued for up to 48 h before the cell died. Blebbing could also be induced by a purified chymotrypsin-like proteinase of T. denticola. Cortical actin and α-actinin of the bacterium- treated cells showed disorganization, and pericellular fibronectin was degraded by both whole T. denticola and the isolated proteinase. Epithelial cells with well-formed lateral cell contacts appeared to be more resistant to the effects of T. denticola than migrating isolated cells. In multilayer epithelial cultures, adhesion of T. denticola and membrane blebbing were observed infrequently. There was no evidence of invasion of T. denticola into epithelial multilayers. However, immunogold electron microscopy showed rapid transport of T. denticola chymotrypsin-like proteinase into newly formed large intracellular vacuoles within the epithelial layers. These vacuoles were lined by membranes studded with ribosomes. T. denticola-treated epithelial multilayers had loose cell contacts, collapsed intercellular spaces, and increased permeability. Through its capacity to cause these unique cytopathic effects, the chymotrypsin-like proteinase of T. denticola has the potential to contribute to the initiation of periodontal disease.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofInfection and Immunityen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsInfection and Immunity. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Infection and Immunity, 1995, v. 63 n. 9, p. 3401-3410en_HK
dc.subject.meshChymotrypsin - toxicityen_HK
dc.subject.meshPeriodontal Ligament - cytology - drug effectsen_HK
dc.subject.meshTreponema - enzymology - immunology - pathogenicityen_HK
dc.subject.meshAntigens, Bacterial - metabolismen_HK
dc.subject.meshCell Movementen_HK
dc.titleCytopathic effects of Treponema denticola chymotrypsin-like proteinase on migrating and stratified epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0019-9567&volume=63&issue=9&spage=3401&epage=3410&date=1995&atitle=Cytopathic+effects+of+Treponema+denticola+chymotrypsin-like+proteinase+on+migrating+and+stratified+epithelial+cellsen_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid7642269-
dc.identifier.pmcidPMC173468-
dc.identifier.scopuseid_2-s2.0-0029112596en_HK
dc.identifier.hkuros13896-
dc.identifier.volume63en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3401en_HK
dc.identifier.epage3410en_HK
dc.identifier.isiWOS:A1995RQ79200023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridUitto, VJ=7004455896en_HK
dc.identifier.scopusauthoridPan, YM=7403340328en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridLarjava, H=7005475246en_HK
dc.identifier.scopusauthoridEllen, RP=7005449768en_HK
dc.identifier.scopusauthoridFinlay, BB=7102051213en_HK
dc.identifier.scopusauthoridMcBride, BC=7102465580en_HK

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