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Article: Unraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model

TitleUnraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion model
Authors
Issue Date2007
PublisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.org
Citation
Journal of Endocrinology, 2007, v. 192 n. 3, p. 563-583 How to Cite?
AbstractDuring spermatogenesis, extensive restructuring takes place at the Sertoli-Sertoli and Sertoli-germ cell interface, which is regulated via intriguing interactions among cytokines, proteases, protease inhibitors, kinases, phosphatases, and transcription factors. This in turn determines the steady-state levels of integral membrane proteins at the cell junctions. We sought to further expand these observations using the Adjudin model. Adjudin is a potential male contraceptive that targets Sertoli-germ cell adhesion, causing exfoliation of spermatids and spermatocytes, but not spermatogonia, from the seminiferous epithelium. This model thus provides the means to identify crucial regulatory molecules and signaling pathways pertinent to junction restructuring events during spermatogenesis. In this study, genome-wide expression profiling of rat testes after treatment with Adjudin at the time of extensive junction restructuring was performed. Differentially regulated genes, such as cytokines, proteases, protease inhibitors, cell junction-associated proteins, and transcription factors pertinent to junction restructuring were identified. These data were consistent with earlier findings; however, much new information was obtained which has been deposited at the Gene Expression Omnibus data repository website: http://www.ncbi.nih.gov/geo/ with Accession number: GSE5131. The primary signaling events pertinent to junction restructuring in the testis induced by Adjudin were also delineated using bioinformatics. These findings were also consistent with recently published reports. The identified molecular signatures or targets pertinent to junction dynamics in the testis as reported herein, many of which have not been investigated, thus offer a framework upon which the regulation of junction restructuring events at the Sertoli-Sertoli and Sertoli-germ cell interface pertinent to spermatogenesis can be further studied.
Persistent Identifierhttp://hdl.handle.net/10722/48724
ISSN
2015 Impact Factor: 4.498
2015 SCImago Journal Rankings: 1.910
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXia, Wen_HK
dc.contributor.authorMruk, DDen_HK
dc.contributor.authorLee, WWMen_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2008-05-22T04:22:27Z-
dc.date.available2008-05-22T04:22:27Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal of Endocrinology, 2007, v. 192 n. 3, p. 563-583en_HK
dc.identifier.issn0022-0795en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48724-
dc.description.abstractDuring spermatogenesis, extensive restructuring takes place at the Sertoli-Sertoli and Sertoli-germ cell interface, which is regulated via intriguing interactions among cytokines, proteases, protease inhibitors, kinases, phosphatases, and transcription factors. This in turn determines the steady-state levels of integral membrane proteins at the cell junctions. We sought to further expand these observations using the Adjudin model. Adjudin is a potential male contraceptive that targets Sertoli-germ cell adhesion, causing exfoliation of spermatids and spermatocytes, but not spermatogonia, from the seminiferous epithelium. This model thus provides the means to identify crucial regulatory molecules and signaling pathways pertinent to junction restructuring events during spermatogenesis. In this study, genome-wide expression profiling of rat testes after treatment with Adjudin at the time of extensive junction restructuring was performed. Differentially regulated genes, such as cytokines, proteases, protease inhibitors, cell junction-associated proteins, and transcription factors pertinent to junction restructuring were identified. These data were consistent with earlier findings; however, much new information was obtained which has been deposited at the Gene Expression Omnibus data repository website: http://www.ncbi.nih.gov/geo/ with Accession number: GSE5131. The primary signaling events pertinent to junction restructuring in the testis induced by Adjudin were also delineated using bioinformatics. These findings were also consistent with recently published reports. The identified molecular signatures or targets pertinent to junction dynamics in the testis as reported herein, many of which have not been investigated, thus offer a framework upon which the regulation of junction restructuring events at the Sertoli-Sertoli and Sertoli-germ cell interface pertinent to spermatogenesis can be further studied.en_HK
dc.format.extent1714769 bytes-
dc.format.extent2457 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherSociety for Endocrinology. The Journal's web site is located at http://joe.endocrinology-journals.orgen_HK
dc.rightsDisclaimer. This is not the definitive version of record of this article. This manuscript has been accepted for publication in Journal of Endocrinology, but the version presented here has not yet been copy edited, formatted or proofed. Consequently, the Society for Endocrinology accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at http://dx.doi.org/10.1677/JOE-06-0158. ©2007 Society for Endocrinologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Endocrinology. Copyright © Society for Endocrinology.-
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshSpermatogenesis - geneticsen_HK
dc.subject.meshContraceptive Agents, Male - pharmacologyen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshHydrazines - pharmacologyen_HK
dc.titleUnraveling the molecular targets pertinent to junction restructuring events during spermatogenesis using the Adjudin-induced germ cell depletion modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0795&volume=192&issue=3&spage=563&epage=583&date=2007&atitle=Unraveling+the+molecular+targets+pertinent+to+junction+restructuring+events+during+spermatogenesis+using+the+Adjudin-induced+germ+cell+depletion+modelen_HK
dc.identifier.emailLee, WWM: hrszlwm@hku.hken_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1677/JOE-06-0158en_HK
dc.identifier.pmid17332525-
dc.identifier.pmcidPMC2804028-
dc.identifier.scopuseid_2-s2.0-33947367483-
dc.identifier.hkuros132222-
dc.identifier.isiWOS:000245123100010-
dc.identifier.scopusauthoridXia, W=8672244100-
dc.identifier.scopusauthoridMruk, DD=6701823934-
dc.identifier.scopusauthoridLee, WM=24799156600-
dc.identifier.scopusauthoridCheng, CY=7404797787-

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