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Article: Molecular diagnostics in tuberculosis

TitleMolecular diagnostics in tuberculosis
Authors
KeywordsMolecular Diagnostic Techniques
Tuberculosis - diagnosis
Mycobacterium tuberculosis - classification - genetics - isolation & purification
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htm
Citation
European Journal Of Clinical Microbiology And Infectious Diseases, 2005, v. 24 n. 11, p. 711-720 How to Cite?
AbstractMolecular diagnostics in tuberculosis has enabled rapid detection of Mycobacterium tuberculosis complex in clinical specimens, identification of mycobacterial species, detection of drug resistance, and typing for epidemiological investigation. In the laboratory diagnosis of tuberculosis, the nucleic acid amplification (NAA) test is rapid and specific but not as sensitive as culture of mycobacteria. The primary determinant of successful NAA testing for tuberculosis depends on the shedding of mycobacterial DNA in secretions from caseating granulomas and its dissemination into sterile body fluids or tissue biopsies. In multibacillary diseases with a high mycobacterial load, a positive Ziehl-Neelsen smear with a positive NAA test is diagnostic of active tuberculosis, whereas a positive Ziehl-Neelsen smear with a negative NAA test in the absence of inhibitors would indicate nontuberculous mycobacterial disease. The role of the NAA test is more important in paucibacillary diseases with low mycobacterial loads. The presence of polymerase chain reaction (PCR) inhibitors, however, especially in extrapulmonary specimens, may produce false-negative results. Although this problem can be overcome to some extent by extra extraction steps, the additional processing invariably leads to the loss of mycobacterial DNA. To circumvent this problem, a brief culture augmentation step is carried out before the NAA test is performed, which can enhance the mycobacterial load while concomitantly diluting inhibitors, thereby maintaining the sensitivity of the test without excessively increasing turnaround time. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/48639
ISSN
2015 Impact Factor: 2.857
2015 SCImago Journal Rankings: 1.173
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheng, VCCen_HK
dc.contributor.authorYew, WWen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-05-22T04:19:46Z-
dc.date.available2008-05-22T04:19:46Z-
dc.date.issued2005en_HK
dc.identifier.citationEuropean Journal Of Clinical Microbiology And Infectious Diseases, 2005, v. 24 n. 11, p. 711-720en_HK
dc.identifier.issn0934-9723en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48639-
dc.description.abstractMolecular diagnostics in tuberculosis has enabled rapid detection of Mycobacterium tuberculosis complex in clinical specimens, identification of mycobacterial species, detection of drug resistance, and typing for epidemiological investigation. In the laboratory diagnosis of tuberculosis, the nucleic acid amplification (NAA) test is rapid and specific but not as sensitive as culture of mycobacteria. The primary determinant of successful NAA testing for tuberculosis depends on the shedding of mycobacterial DNA in secretions from caseating granulomas and its dissemination into sterile body fluids or tissue biopsies. In multibacillary diseases with a high mycobacterial load, a positive Ziehl-Neelsen smear with a positive NAA test is diagnostic of active tuberculosis, whereas a positive Ziehl-Neelsen smear with a negative NAA test in the absence of inhibitors would indicate nontuberculous mycobacterial disease. The role of the NAA test is more important in paucibacillary diseases with low mycobacterial loads. The presence of polymerase chain reaction (PCR) inhibitors, however, especially in extrapulmonary specimens, may produce false-negative results. Although this problem can be overcome to some extent by extra extraction steps, the additional processing invariably leads to the loss of mycobacterial DNA. To circumvent this problem, a brief culture augmentation step is carried out before the NAA test is performed, which can enhance the mycobacterial load while concomitantly diluting inhibitors, thereby maintaining the sensitivity of the test without excessively increasing turnaround time. © Springer-Verlag 2005.en_HK
dc.format.extent122025 bytes-
dc.format.extent1902 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/10096/index.htmen_HK
dc.relation.ispartofEuropean Journal of Clinical Microbiology and Infectious Diseasesen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMolecular Diagnostic Techniquesen_HK
dc.subjectTuberculosis - diagnosisen_HK
dc.subjectMycobacterium tuberculosis - classification - genetics - isolation & purificationen_HK
dc.titleMolecular diagnostics in tuberculosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0934-9723&volume=24&issue=11&spage=711&epage=720&date=2005&atitle=Molecular+diagnostics+in+tuberculosisen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1007/s10096-005-0039-1en_HK
dc.identifier.pmid16283213-
dc.identifier.scopuseid_2-s2.0-28644442966en_HK
dc.identifier.hkuros117188-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28644442966&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue11en_HK
dc.identifier.spage711en_HK
dc.identifier.epage720en_HK
dc.identifier.isiWOS:000233736700001-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridCheng, VCC=23670479400en_HK
dc.identifier.scopusauthoridYew, WW=7005934631en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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