Article: Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5′ end of the viral intron

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TitleGeneration of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5′ end of the viral intron
AuthorsCheung, TKW1
Guan, Y1
Ng, SSF1
Chen, H1
Wong, CHK1
Peiris, JSM1
Poon, LLM1
Issue Date2005
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
CitationJournal Of General Virology, 2005, v. 86 n. 5, p. 1447-1454 [How to Cite?]
DOI: http://dx.doi.org/10.1099/vir.0.80727-0
AbstractThe aim of this study was to inhibit influenza virus M2 protein expression by mutating the splicing signal of the M gene. Mutations were introduced into the GU dinucleotide sequence at the 5′-proximal splicing site of the M gene (corresponding to nt 52-53 of M cRNA). Transfected cells expressing mutated M viral ribonucleoproteins failed to generate M2 mRNA. Interestingly, recombinant viruses with mutations at the dinucleotide sequence were viable, albeit attenuated, in cell culture. These recombinants failed to express M2 mRNA and M2 protein. These observations demonstrated that the GU invariant dinucleotide sequence at the 5′-proximal splicing site of M gene is essential for M2 mRNA synthesis. These results also indicated that the M2 ion-channel protein is critical, but not essential, for virus replication in cell culture. This approach may provide a new way of producing attenuated influenza A virus. © 2005 SGM.
ISSN0022-1317
2011 Impact Factor: 3.363
2011 SCImago Journal Rankings: 0.360
DOIhttp://dx.doi.org/10.1099/vir.0.80727-0
ISI Accession Number IDWOS:000228717200024
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCheung, TKW
dc.contributor.authorGuan, Y
dc.contributor.authorNg, SSF
dc.contributor.authorChen, H
dc.contributor.authorWong, CHK
dc.contributor.authorPeiris, JSM
dc.contributor.authorPoon, LLM
dc.date.accessioned2008-05-22T04:19:43Z
dc.date.available2008-05-22T04:19:43Z
dc.date.issued2005
dc.description.abstractThe aim of this study was to inhibit influenza virus M2 protein expression by mutating the splicing signal of the M gene. Mutations were introduced into the GU dinucleotide sequence at the 5′-proximal splicing site of the M gene (corresponding to nt 52-53 of M cRNA). Transfected cells expressing mutated M viral ribonucleoproteins failed to generate M2 mRNA. Interestingly, recombinant viruses with mutations at the dinucleotide sequence were viable, albeit attenuated, in cell culture. These recombinants failed to express M2 mRNA and M2 protein. These observations demonstrated that the GU invariant dinucleotide sequence at the 5′-proximal splicing site of M gene is essential for M2 mRNA synthesis. These results also indicated that the M2 ion-channel protein is critical, but not essential, for virus replication in cell culture. This approach may provide a new way of producing attenuated influenza A virus. © 2005 SGM.
dc.description.naturepostprint
dc.format.extent322748 bytes
dc.format.extent110067 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.identifier.citationJournal Of General Virology, 2005, v. 86 n. 5, p. 1447-1454 [How to Cite?]
DOI: http://dx.doi.org/10.1099/vir.0.80727-0
dc.identifier.doihttp://dx.doi.org/10.1099/vir.0.80727-0
dc.identifier.epage1454
dc.identifier.isiWOS:000228717200024
dc.identifier.issn0022-1317
2011 Impact Factor: 3.363
2011 SCImago Journal Rankings: 0.360
dc.identifier.issue5
dc.identifier.openurl
dc.identifier.pmid15831957
dc.identifier.scopuseid_2-s2.0-18144385402
dc.identifier.spage1447
dc.identifier.urihttp://hdl.handle.net/10722/48637
dc.identifier.volume86
dc.languageeng
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of General Virology
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshInfluenza a virus
dc.subject.meshChemistry
dc.subject.meshGenetics
dc.subject.meshPoint mutation
dc.subject.meshIntrons
dc.titleGeneration of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5′ end of the viral intron
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong