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Article: Proteomic analysis of a preneoplastic phenotype in ovarian surface epithelial cells derived from prophylactic oophorectomies

TitleProteomic analysis of a preneoplastic phenotype in ovarian surface epithelial cells derived from prophylactic oophorectomies
Authors
KeywordsBRCA1
Hereditary
Ovarian cancers
Ovarian surface epithelium
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2005, v. 98 n. 1, p. 68-76 How to Cite?
AbstractObjective. To study the pattern of protein expression associated with a predisposition to develop ovarian cancer. Methods. Prophylactic oophorectomy is used to prevent ovarian carcinoma in high-risk populations who have a strong family history of breast/ovarian cancer. In ovarian specimens of these women, the ovarian surface epithelium (OSE), which is tissue of origin of epithelial ovarian cancer, often shows altered morphology, growth patterns and differentiation features that are believed to be preneoplastic. This study has used a proteomic approach, based on two-dimensional gel electrophoresis and mass spectrometry, to compare the protein profiles of OSE from women with a history of familial ovarian cancer (FH-OSE), i.e., at least two first-degree relatives with such cancer and/or testing positive for BRCA1 mutations, to those without such history (NFH-OSE). Results. Of >1500 protein spots, there were 8 proteins whose levels were significantly altered in FH-OSE. Three were known ovarian tumor associated proteins, others were novel changes. A number of the alterations seen were accompanied with protein modifications and have not been previously reported. There was a predominance of sequences related to the stress response pathway. Differential expression of selected genes was confirmed by Western blotting and real-time reverse transcription polymerase chain reaction. Conclusions. Our findings define the OSE phenotype of women at a high risk of developing ovarian cancer. Protein alterations seen in these tissues may represent an early, irreversible, non-mutational step in ovarian epithelial neoplastic progression and may be potential early and sensitive markers for the evaluation of cancer risk. © 2005 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/48511
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, QYen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorWong, Een_HK
dc.contributor.authorEhlen, TGen_HK
dc.contributor.authorAuersperg, Nen_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorWong, ASTen_HK
dc.date.accessioned2008-05-22T04:15:52Z-
dc.date.available2008-05-22T04:15:52Z-
dc.date.issued2005en_HK
dc.identifier.citationGynecologic Oncology, 2005, v. 98 n. 1, p. 68-76en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48511-
dc.description.abstractObjective. To study the pattern of protein expression associated with a predisposition to develop ovarian cancer. Methods. Prophylactic oophorectomy is used to prevent ovarian carcinoma in high-risk populations who have a strong family history of breast/ovarian cancer. In ovarian specimens of these women, the ovarian surface epithelium (OSE), which is tissue of origin of epithelial ovarian cancer, often shows altered morphology, growth patterns and differentiation features that are believed to be preneoplastic. This study has used a proteomic approach, based on two-dimensional gel electrophoresis and mass spectrometry, to compare the protein profiles of OSE from women with a history of familial ovarian cancer (FH-OSE), i.e., at least two first-degree relatives with such cancer and/or testing positive for BRCA1 mutations, to those without such history (NFH-OSE). Results. Of >1500 protein spots, there were 8 proteins whose levels were significantly altered in FH-OSE. Three were known ovarian tumor associated proteins, others were novel changes. A number of the alterations seen were accompanied with protein modifications and have not been previously reported. There was a predominance of sequences related to the stress response pathway. Differential expression of selected genes was confirmed by Western blotting and real-time reverse transcription polymerase chain reaction. Conclusions. Our findings define the OSE phenotype of women at a high risk of developing ovarian cancer. Protein alterations seen in these tissues may represent an early, irreversible, non-mutational step in ovarian epithelial neoplastic progression and may be potential early and sensitive markers for the evaluation of cancer risk. © 2005 Elsevier Inc. All rights reserved.en_HK
dc.format.extent70427 bytes-
dc.format.extent254114 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBRCA1en_HK
dc.subjectHereditaryen_HK
dc.subjectOvarian cancersen_HK
dc.subjectOvarian surface epitheliumen_HK
dc.titleProteomic analysis of a preneoplastic phenotype in ovarian surface epithelial cells derived from prophylactic oophorectomiesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=98&spage=68&epage=76&date=2005&atitle=Proteomic+analysis+of+a+preneoplastic+phenotype+in+ovarian+surface+epithelial+cells+derived+from+prophylactic+oophorectomiesen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.ygyno.2005.04.002en_HK
dc.identifier.pmid15913737-
dc.identifier.scopuseid_2-s2.0-20444502473en_HK
dc.identifier.hkuros101945-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20444502473&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume98en_HK
dc.identifier.issue1en_HK
dc.identifier.spage68en_HK
dc.identifier.epage76en_HK
dc.identifier.isiWOS:000230284200012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHe, QY=34770287900en_HK
dc.identifier.scopusauthoridZhou, Y=7405366890en_HK
dc.identifier.scopusauthoridWong, E=23101622300en_HK
dc.identifier.scopusauthoridEhlen, TG=6603203782en_HK
dc.identifier.scopusauthoridAuersperg, N=7006582556en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridWong, AST=23987963300en_HK

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