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Article: Inducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco

TitleInducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco
Authors
Keywordsβ-Glucuronidase
dsRNA
Ethanol induction
Gene silencing
Issue Date2005
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 2005, v. 579 n. 6, p. 1498-1502 How to Cite?
AbstractA binary vector amenable to high-throughput cloning was constructed for ethanol-inducible expression of double-stranded RNA (dsRNA) in plants. Silencing of a transgene encoding β-glucuronidase (GUS) was then examined at RNA and protein levels in tobacco. Transient gene silencing could be effectively achieved in plants with higher expression levels of alcR (the ethanol sensor) after single application of 1% ethanol (v/v) through root drenching. GUS activities showed more dramatic pattern of loss and recovery in young leaves than in older leaves. Repeated ethanol treatment resulted in extended gene suppression and increased loss of GUS activities. Interestingly, recovery of GUS transcript level is dramatically earlier than that of GUS protein levels as measured by enzyme assays. These observations indicate that dsRNA-mediated gene silencing may occur through more stable translational inhibition in addition to reversible targeted RNA degradation. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/48508
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, Cen_HK
dc.contributor.authorWang, Nen_HK
dc.contributor.authorLam, Een_HK
dc.date.accessioned2008-05-22T04:15:43Z-
dc.date.available2008-05-22T04:15:43Z-
dc.date.issued2005en_HK
dc.identifier.citationFebs Letters, 2005, v. 579 n. 6, p. 1498-1502en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48508-
dc.description.abstractA binary vector amenable to high-throughput cloning was constructed for ethanol-inducible expression of double-stranded RNA (dsRNA) in plants. Silencing of a transgene encoding β-glucuronidase (GUS) was then examined at RNA and protein levels in tobacco. Transient gene silencing could be effectively achieved in plants with higher expression levels of alcR (the ethanol sensor) after single application of 1% ethanol (v/v) through root drenching. GUS activities showed more dramatic pattern of loss and recovery in young leaves than in older leaves. Repeated ethanol treatment resulted in extended gene suppression and increased loss of GUS activities. Interestingly, recovery of GUS transcript level is dramatically earlier than that of GUS protein levels as measured by enzyme assays. These observations indicate that dsRNA-mediated gene silencing may occur through more stable translational inhibition in addition to reversible targeted RNA degradation. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.en_HK
dc.format.extent259629 bytes-
dc.format.extent235804 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.languagefreen_HK
dc.languagegeren_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectβ-Glucuronidaseen_HK
dc.subjectdsRNAen_HK
dc.subjectEthanol inductionen_HK
dc.subjectGene silencingen_HK
dc.titleInducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobaccoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=579&spage=1498&epage=1502&date=2005&atitle=Inducible+double-stranded+RNA+expression+activates+reversible+transcript+turnover+and+stable+translational+suppression+of+a+target+gene+in+transgenic+tobaccoen_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.febslet.2005.01.062en_HK
dc.identifier.pmid15733863-
dc.identifier.scopuseid_2-s2.0-14244251254en_HK
dc.identifier.hkuros98230-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14244251254&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume579en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1498en_HK
dc.identifier.epage1502en_HK
dc.identifier.isiWOS:000227393700029-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK
dc.identifier.scopusauthoridWang, N=55467281000en_HK
dc.identifier.scopusauthoridLam, E=7102890014en_HK

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