Article: Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells

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TitleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
AuthorsZhou, Y1
Chan, JH1
Chan, AY1
Chak, RKF1
Wong, EYL1
Chye, ML1
Peiris, JSM1
Poon, LLM1
Lam, E1
KeywordsEYFP, gene encoding the enhanced yellow fluorescent protein
MDCK, Madin-Darby canine kidney
NS1, gene encoding the non-structural protein NS1
PTGS, post-transcriptional gene silencing
RNAi, RNA interference
siRNA, small-interfering RNA
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
CitationFebs Letters, 2004, v. 577 n. 3, p. 345-350 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.febslet.2004.10.027
AbstractAs an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
ISSN0014-5793
2011 Impact Factor: 3.538
2011 SCImago Journal Rankings: 0.484
DOIhttp://dx.doi.org/10.1016/j.febslet.2004.10.027
ISI Accession Number IDWOS:000225368300006
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorZhou, Y
dc.contributor.authorChan, JH
dc.contributor.authorChan, AY
dc.contributor.authorChak, RKF
dc.contributor.authorWong, EYL
dc.contributor.authorChye, ML
dc.contributor.authorPeiris, JSM
dc.contributor.authorPoon, LLM
dc.contributor.authorLam, E
dc.date.accessioned2008-05-22T04:15:41Z
dc.date.available2008-05-22T04:15:41Z
dc.date.issued2004
dc.description.abstractAs an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
dc.description.naturepostprint
dc.format.extent324184 bytes
dc.format.extent56832 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/msword
dc.identifier.citationFebs Letters, 2004, v. 577 n. 3, p. 345-350 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.febslet.2004.10.027
dc.identifier.doihttp://dx.doi.org/10.1016/j.febslet.2004.10.027
dc.identifier.epage350
dc.identifier.hkuros96621
dc.identifier.isiWOS:000225368300006
dc.identifier.issn0014-5793
2011 Impact Factor: 3.538
2011 SCImago Journal Rankings: 0.484
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid15556607
dc.identifier.scopuseid_2-s2.0-8844252335
dc.identifier.spage345
dc.identifier.urihttp://hdl.handle.net/10722/48507
dc.identifier.volume577
dc.languageeng
dc.languagefre
dc.languageger
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
dc.publisher.placeNetherlands
dc.relation.ispartofFEBS Letters
dc.relation.referencesReferences in Scopus
dc.rightsF E B S Letters. Copyright © Elsevier BV.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subjectEYFP, gene encoding the enhanced yellow fluorescent protein
dc.subjectMDCK, Madin-Darby canine kidney
dc.subjectNS1, gene encoding the non-structural protein NS1
dc.subjectPTGS, post-transcriptional gene silencing
dc.subjectRNAi, RNA interference
dc.subjectsiRNA, small-interfering RNA
dc.titleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong