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Article: Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
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TitleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
 
AuthorsZhou, Y1
Chan, JH1
Chan, AY1
Chak, RKF1
Wong, EYL1
Chye, ML1
Peiris, JSM1
Poon, LLM1
Lam, E1
 
KeywordsEYFP, gene encoding the enhanced yellow fluorescent protein
MDCK, Madin-Darby canine kidney
NS1, gene encoding the non-structural protein NS1
PTGS, post-transcriptional gene silencing
RNAi, RNA interference
siRNA, small-interfering RNA
 
Issue Date2004
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
 
CitationFebs Letters, 2004, v. 577 n. 3, p. 345-350 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.febslet.2004.10.027
 
AbstractAs an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
 
ISSN0014-5793
2012 Impact Factor: 3.582
2012 SCImago Journal Rankings: 1.848
 
DOIhttp://dx.doi.org/10.1016/j.febslet.2004.10.027
 
ISI Accession Number IDWOS:000225368300006
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhou, Y
 
dc.contributor.authorChan, JH
 
dc.contributor.authorChan, AY
 
dc.contributor.authorChak, RKF
 
dc.contributor.authorWong, EYL
 
dc.contributor.authorChye, ML
 
dc.contributor.authorPeiris, JSM
 
dc.contributor.authorPoon, LLM
 
dc.contributor.authorLam, E
 
dc.date.accessioned2008-05-22T04:15:41Z
 
dc.date.available2008-05-22T04:15:41Z
 
dc.date.issued2004
 
dc.description.abstractAs an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
 
dc.description.naturepostprint
 
dc.format.extent324184 bytes
 
dc.format.extent56832 bytes
 
dc.format.mimetypeapplication/pdf
 
dc.format.mimetypeapplication/msword
 
dc.identifier.citationFebs Letters, 2004, v. 577 n. 3, p. 345-350 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.febslet.2004.10.027
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.febslet.2004.10.027
 
dc.identifier.epage350
 
dc.identifier.hkuros96621
 
dc.identifier.isiWOS:000225368300006
 
dc.identifier.issn0014-5793
2012 Impact Factor: 3.582
2012 SCImago Journal Rankings: 1.848
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid15556607
 
dc.identifier.scopuseid_2-s2.0-8844252335
 
dc.identifier.spage345
 
dc.identifier.urihttp://hdl.handle.net/10722/48507
 
dc.identifier.volume577
 
dc.languageeng
 
dc.languagefre
 
dc.languageger
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofFEBS Letters
 
dc.relation.referencesReferences in Scopus
 
dc.rightsF E B S Letters. Copyright © Elsevier BV.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectEYFP, gene encoding the enhanced yellow fluorescent protein
 
dc.subjectMDCK, Madin-Darby canine kidney
 
dc.subjectNS1, gene encoding the non-structural protein NS1
 
dc.subjectPTGS, post-transcriptional gene silencing
 
dc.subjectRNAi, RNA interference
 
dc.subjectsiRNA, small-interfering RNA
 
dc.titleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong