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Article: Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells

TitleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
Authors
KeywordsEYFP, gene encoding the enhanced yellow fluorescent protein
MDCK, Madin-Darby canine kidney
NS1, gene encoding the non-structural protein NS1
PTGS, post-transcriptional gene silencing
RNAi, RNA interference
siRNA, small-interfering RNA
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 2004, v. 577 n. 3, p. 345-350 How to Cite?
Abstract
As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Persistent Identifierhttp://hdl.handle.net/10722/48507
ISSN
2013 Impact Factor: 3.341
2013 SCImago Journal Rankings: 2.342
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorChan, JHen_HK
dc.contributor.authorChan, AYen_HK
dc.contributor.authorChak, RKFen_HK
dc.contributor.authorWong, EYLen_HK
dc.contributor.authorChye, MLen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorLam, Een_HK
dc.date.accessioned2008-05-22T04:15:41Z-
dc.date.available2008-05-22T04:15:41Z-
dc.date.issued2004en_HK
dc.identifier.citationFebs Letters, 2004, v. 577 n. 3, p. 345-350en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48507-
dc.description.abstractAs an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5 ′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. © 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.en_HK
dc.format.extent324184 bytes-
dc.format.extent56832 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.languagefreen_HK
dc.languagegeren_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEYFP, gene encoding the enhanced yellow fluorescent proteinen_HK
dc.subjectMDCK, Madin-Darby canine kidneyen_HK
dc.subjectNS1, gene encoding the non-structural protein NS1en_HK
dc.subjectPTGS, post-transcriptional gene silencingen_HK
dc.subjectRNAi, RNA interferenceen_HK
dc.subjectsiRNA, small-interfering RNAen_HK
dc.titleTransgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=577&issue=3&spage=345&epage=350&date=2004&atitle=Transgenic+plant-derived+siRNAs+can+suppress+propagation+of+influenza+virus+in+mammalian+cellsen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.febslet.2004.10.027en_HK
dc.identifier.pmid15556607en_HK
dc.identifier.scopuseid_2-s2.0-8844252335en_HK
dc.identifier.hkuros96621-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-8844252335&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume577en_HK
dc.identifier.issue3en_HK
dc.identifier.spage345en_HK
dc.identifier.epage350en_HK
dc.identifier.isiWOS:000225368300006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhou, Y=23136063700en_HK
dc.identifier.scopusauthoridChan, JH=55230702600en_HK
dc.identifier.scopusauthoridChan, AY=26021082600en_HK
dc.identifier.scopusauthoridChak, RKF=7801603929en_HK
dc.identifier.scopusauthoridWong, EYL=8234468400en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridLam, E=7102890014en_HK

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