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Article: The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi

TitleThe bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi
Authors
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2003, v. 31 n. 16, p. 4822-4827 How to Cite?
AbstractTAGKO is a Tn7-based transposition system for genome wide mutagenesis in filamentous fungi. The effects of transposon insertion on the expression of TAGKO alleles were examined in Magnaporthe grisea and Mycosphaerella graminicola. Northern analysis showed that stable, truncated transcripts were expressed in the TAGKO mutants. Mapping of the 3′-ends of TAGKO cDNAs revealed that they all contain Tn7 end sequences, regardless of the transposon orientation. Polyadenylation signals characteristic of eukaryotic genes, preceded by stop codons in all frames, are located in both ends of the bacterial transposon. Thus, TAGKO transcripts are prematurely polyadenylated, and truncated proteins are predicted to be translated in the fungal mutants. Depending on the extent of protein truncation, TAGKO mutations in HPD4 (encoding p-hydroxyphenylpyruvate dioxygenase) resulted in tyrosine sensitivity in the two fungi. Similarly, a particular M.grisea CBS1 (encoding cystathionine β-synthase) TAGKO cDNA failed to complement cysteine auxotrophy in a yeast CBS mutant. TAGKO, therefore, represents a useful tool for in vivo study of truncated gene products in filamentous fungi.
Persistent Identifierhttp://hdl.handle.net/10722/48506
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, Cen_HK
dc.contributor.authorAdachi, Ken_HK
dc.contributor.authorShuster, JRen_HK
dc.contributor.authorHamer, JEen_HK
dc.contributor.authorHamer, Len_HK
dc.date.accessioned2008-05-22T04:15:39Z-
dc.date.available2008-05-22T04:15:39Z-
dc.date.issued2003en_HK
dc.identifier.citationNucleic Acids Research, 2003, v. 31 n. 16, p. 4822-4827en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48506-
dc.description.abstractTAGKO is a Tn7-based transposition system for genome wide mutagenesis in filamentous fungi. The effects of transposon insertion on the expression of TAGKO alleles were examined in Magnaporthe grisea and Mycosphaerella graminicola. Northern analysis showed that stable, truncated transcripts were expressed in the TAGKO mutants. Mapping of the 3′-ends of TAGKO cDNAs revealed that they all contain Tn7 end sequences, regardless of the transposon orientation. Polyadenylation signals characteristic of eukaryotic genes, preceded by stop codons in all frames, are located in both ends of the bacterial transposon. Thus, TAGKO transcripts are prematurely polyadenylated, and truncated proteins are predicted to be translated in the fungal mutants. Depending on the extent of protein truncation, TAGKO mutations in HPD4 (encoding p-hydroxyphenylpyruvate dioxygenase) resulted in tyrosine sensitivity in the two fungi. Similarly, a particular M.grisea CBS1 (encoding cystathionine β-synthase) TAGKO cDNA failed to complement cysteine auxotrophy in a yeast CBS mutant. TAGKO, therefore, represents a useful tool for in vivo study of truncated gene products in filamentous fungi.en_HK
dc.format.extent1683543 bytes-
dc.format.extent235804 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAscomycota - enzymology - geneticsen_HK
dc.subject.meshDNA Transposable Elements - geneticsen_HK
dc.subject.meshEukaryotic Cells - metabolismen_HK
dc.subject.meshPoly A - genetics - metabolismen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.titleThe bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1048&volume=31&issue=16&spage=4822&epage=4827&date=2003&atitle=The+bacterial+transposon+Tn7+causes+premature+polyadenylation+of+mRNA+in+eukaryotic+organisms:+TAGKO+mutagenesis+in+filamentous+fungien_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1093/nar/gkg676en_HK
dc.identifier.pmid12907724-
dc.identifier.pmcidPMC169947en_HK
dc.identifier.scopuseid_2-s2.0-0344875521en_HK
dc.identifier.hkuros81897-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344875521&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue16en_HK
dc.identifier.spage4822en_HK
dc.identifier.epage4827en_HK
dc.identifier.isiWOS:000184783000019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK
dc.identifier.scopusauthoridAdachi, K=7401602553en_HK
dc.identifier.scopusauthoridShuster, JR=7201487088en_HK
dc.identifier.scopusauthoridHamer, JE=7102288376en_HK
dc.identifier.scopusauthoridHamer, L=6603685512en_HK

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