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Article: Molecular characterization and in silico expression analysis of a chalcone synthase gene family in Sorghum bicolor

TitleMolecular characterization and in silico expression analysis of a chalcone synthase gene family in Sorghum bicolor
Authors
KeywordsChalcone synthase
In silico gene expression analysis
Sorghum bicolor
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/pmpp
Citation
Physiological And Molecular Plant Pathology, 2002, v. 61 n. 3, p. 179-188 How to Cite?
AbstractRecent use of Sorghum bicolor as a target for grass genomics has presented new resources for gene discovery in novel metabolic pathways in Poaceae. Sorghum synthesizes a unique class of flavonoid phytoalexins, the 3-deoxyanthocyanidins, in response to fungal infection. The biosynthetic pathways for 3-deoxyflavonoids are largely uncharacterized but are known to involve transcriptional activation of chalcone synthase (CHS). CHS, or naringenin CHS, catalyses the formation of naringenin, the precursor for different flavonoids. We have isolated seven sorghum CHS genes, CHS1-7, from a genomic library on high-density filters. CHS1-7 are highly conserved and closely related to the maize C2 and Whp genes. Several of them are also linked in the genome. These findings suggest that they are the result of recent gene duplication events. Expression of the individual CHS genes was studied in silico by examination of expressed sequence tag (EST) data available in the public domain. Our analyses suggested that CHS1-7 were not differentially expressed in the various growth and developmental conditions represented by the cDNA libraries used to generate the EST data. However, we identified a CHS-like gene, CHS8, with significantly higher EST abundance in the pathogen-induced library. CHS8 shows only 81-82% identity to CHS1-7 and forms a distinct subgroup in our phylogenetic analysis. In addition, the active site region contains substitutions that distinguish CHS8 from naringenin CHS. We propose that CHS8 has evolved new enzymatic functions that are involved in the synthesis of defence-related flavonoids, such as the 3-deoxyanthocyanidins, during fungal infection. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/48505
ISSN
2015 Impact Factor: 1.371
2015 SCImago Journal Rankings: 0.613
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, Cen_HK
dc.contributor.authorCoolbaugh, RCen_HK
dc.contributor.authorNicholson, RLen_HK
dc.date.accessioned2008-05-22T04:15:38Z-
dc.date.available2008-05-22T04:15:38Z-
dc.date.issued2002en_HK
dc.identifier.citationPhysiological And Molecular Plant Pathology, 2002, v. 61 n. 3, p. 179-188en_HK
dc.identifier.issn0885-5765en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48505-
dc.description.abstractRecent use of Sorghum bicolor as a target for grass genomics has presented new resources for gene discovery in novel metabolic pathways in Poaceae. Sorghum synthesizes a unique class of flavonoid phytoalexins, the 3-deoxyanthocyanidins, in response to fungal infection. The biosynthetic pathways for 3-deoxyflavonoids are largely uncharacterized but are known to involve transcriptional activation of chalcone synthase (CHS). CHS, or naringenin CHS, catalyses the formation of naringenin, the precursor for different flavonoids. We have isolated seven sorghum CHS genes, CHS1-7, from a genomic library on high-density filters. CHS1-7 are highly conserved and closely related to the maize C2 and Whp genes. Several of them are also linked in the genome. These findings suggest that they are the result of recent gene duplication events. Expression of the individual CHS genes was studied in silico by examination of expressed sequence tag (EST) data available in the public domain. Our analyses suggested that CHS1-7 were not differentially expressed in the various growth and developmental conditions represented by the cDNA libraries used to generate the EST data. However, we identified a CHS-like gene, CHS8, with significantly higher EST abundance in the pathogen-induced library. CHS8 shows only 81-82% identity to CHS1-7 and forms a distinct subgroup in our phylogenetic analysis. In addition, the active site region contains substitutions that distinguish CHS8 from naringenin CHS. We propose that CHS8 has evolved new enzymatic functions that are involved in the synthesis of defence-related flavonoids, such as the 3-deoxyanthocyanidins, during fungal infection. © 2002 Elsevier Science Ltd. All rights reserved.en_HK
dc.format.extent149997 bytes-
dc.format.extent235804 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/pmppen_HK
dc.relation.ispartofPhysiological and Molecular Plant Pathologyen_HK
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectChalcone synthaseen_HK
dc.subjectIn silico gene expression analysisen_HK
dc.subjectSorghum bicoloren_HK
dc.titleMolecular characterization and in silico expression analysis of a chalcone synthase gene family in Sorghum bicoloren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0885-5765&volume=61&spage=179&epage=188&date=2002&atitle=Molecular+characterization+and+in+silico+expression+analysis+of+a+chalcone+synthase+gene+family+in+Sorghum+bicoloren_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1006/pmpp.2002.0428en_HK
dc.identifier.scopuseid_2-s2.0-0036930213en_HK
dc.identifier.hkuros81086-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036930213&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume61en_HK
dc.identifier.issue3en_HK
dc.identifier.spage179en_HK
dc.identifier.epage188en_HK
dc.identifier.isiWOS:000179890700004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK
dc.identifier.scopusauthoridCoolbaugh, RC=6602857913en_HK
dc.identifier.scopusauthoridNicholson, RL=7201624684en_HK

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