File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis

TitleStress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis
Authors
Keywordsβ-Carotene ketolase
Astaxanthin
Haematococcus pluvialis
Physiological conditions
Real-time PCR
Issue Date2006
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotec
Citation
Journal Of Biotechnology, 2006, v. 122 n. 2, p. 176-185 How to Cite?
AbstractThe unicellular green alga Haematococcus pluvialis is used as a biological production system for astaxanthin. It accumulates large amounts of this commercially interesting ketocarotenoid under a variety of environmental stresses. Here we report the identification and expression of three different β-carotene ketolase genes (bkt) that are involved in the biosynthesis of astaxanthin in a single strain of the alga. Bkt1 and bkt2 proved to be the crtO and bkt previously isolated from two different strains of H. pluvialis. Bkt3 is a novel third gene, which shared 95% identical nucleotide sequence with bkt2. Nitrogen deficiency alone could not induce the alga cells to produce astaxanthin in 3 days even though it enhances the expression of the bkt genes to three times of that in normal growing cells within 16 h. High light irradiation (125 μmol m -2 s -1) or 45 mM sodium acetate greatly increased the expression of bkt genes to 18 or 52 times of that in normal growing cells, resulting in an accumulation of substantial astaxanthin (about 6 mg g -1 dry biomass) in 3 days. It is suggested that the existence of the multiple bkt genes and their strong up-regulation by different stress conditions is one of the reasons that H. pluvialis accumulates large amounts of astaxanthin in an instant response to stress environments. © 2005 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/48501
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.064
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, JCen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorSandmann, Gen_HK
dc.date.accessioned2008-05-22T04:15:30Z-
dc.date.available2008-05-22T04:15:30Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Biotechnology, 2006, v. 122 n. 2, p. 176-185en_HK
dc.identifier.issn0168-1656en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48501-
dc.description.abstractThe unicellular green alga Haematococcus pluvialis is used as a biological production system for astaxanthin. It accumulates large amounts of this commercially interesting ketocarotenoid under a variety of environmental stresses. Here we report the identification and expression of three different β-carotene ketolase genes (bkt) that are involved in the biosynthesis of astaxanthin in a single strain of the alga. Bkt1 and bkt2 proved to be the crtO and bkt previously isolated from two different strains of H. pluvialis. Bkt3 is a novel third gene, which shared 95% identical nucleotide sequence with bkt2. Nitrogen deficiency alone could not induce the alga cells to produce astaxanthin in 3 days even though it enhances the expression of the bkt genes to three times of that in normal growing cells within 16 h. High light irradiation (125 μmol m -2 s -1) or 45 mM sodium acetate greatly increased the expression of bkt genes to 18 or 52 times of that in normal growing cells, resulting in an accumulation of substantial astaxanthin (about 6 mg g -1 dry biomass) in 3 days. It is suggested that the existence of the multiple bkt genes and their strong up-regulation by different stress conditions is one of the reasons that H. pluvialis accumulates large amounts of astaxanthin in an instant response to stress environments. © 2005 Elsevier B.V. All rights reserved.en_HK
dc.format.extent268392 bytes-
dc.format.extent3386 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jbiotecen_HK
dc.relation.ispartofJournal of Biotechnologyen_HK
dc.rightsJournal of Biotechnology. Copyright © Elsevier BV.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectβ-Carotene ketolaseen_HK
dc.subjectAstaxanthinen_HK
dc.subjectHaematococcus pluvialisen_HK
dc.subjectPhysiological conditionsen_HK
dc.subjectReal-time PCRen_HK
dc.titleStress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-1656&volume=122&issue=2&spage=176&epage=185&date=2006&atitle=Stress-related+differential+expression+of+multiple+beta-carotene+ketolase+genes+in+the+unicellular+green+alga+Haematococcus+pluvialisen_HK
dc.identifier.emailHuang, JC: huangjc@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityHuang, JC=rp00710en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.jbiotec.2005.09.002en_HK
dc.identifier.pmid16242201-
dc.identifier.scopuseid_2-s2.0-33644550277en_HK
dc.identifier.hkuros122418-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644550277&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue2en_HK
dc.identifier.spage176en_HK
dc.identifier.epage185en_HK
dc.identifier.isiWOS:000236225900003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHuang, JC=7408108735en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridSandmann, G=7006654333en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats