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Article: Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)

TitleIsolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)
Authors
Issue Date2006
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2006, v. 71 n. 4, p. 473-479 How to Cite?
AbstractThe green alga Chlorella zofingiensis produces large amounts of the valuable ketocarotenoid astaxanthin under dark, heterotrophic growth conditions, making it potentially employable for commercial production of astaxanthin as feed additives, colorants, and health products. Here, we report the identification and characterization of a β-carotene oxygenase (CRTO) gene that is directly involved in the biosynthesis of ketocarotenoids in C. zofingiensis. The open reading frame of the crtO gene, which is interrupted by three introns of 243, 318, and 351 bp, respectively, encodes a polypeptide of 312 amino acid residues. Only one crtO gene was detected in the genome of C. zofingiensis. Furthermore, the expression of the crtO gene was transiently up-regulated upon glucose treatment. Functional complementation in Escherichia coli showed that the coding protein of the crtO gene not only exhibits normal CRTO activity by converting β-carotene to canthaxanthin via echinenone, but also displays a high enzymatic activity of converting zeaxanthin to astaxanthin via adonixanthin. Based on the bifunctional CRTO, a predicted pathway for astaxanthin biosynthesis in C. zofingiensis is described, and the CRTO is termed as carotenoid 4,4′-β-ionone ring oxygenase. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/48500
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, JCen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorSandmann, Gen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2008-05-22T04:15:28Z-
dc.date.available2008-05-22T04:15:28Z-
dc.date.issued2006en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2006, v. 71 n. 4, p. 473-479en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/48500-
dc.description.abstractThe green alga Chlorella zofingiensis produces large amounts of the valuable ketocarotenoid astaxanthin under dark, heterotrophic growth conditions, making it potentially employable for commercial production of astaxanthin as feed additives, colorants, and health products. Here, we report the identification and characterization of a β-carotene oxygenase (CRTO) gene that is directly involved in the biosynthesis of ketocarotenoids in C. zofingiensis. The open reading frame of the crtO gene, which is interrupted by three introns of 243, 318, and 351 bp, respectively, encodes a polypeptide of 312 amino acid residues. Only one crtO gene was detected in the genome of C. zofingiensis. Furthermore, the expression of the crtO gene was transiently up-regulated upon glucose treatment. Functional complementation in Escherichia coli showed that the coding protein of the crtO gene not only exhibits normal CRTO activity by converting β-carotene to canthaxanthin via echinenone, but also displays a high enzymatic activity of converting zeaxanthin to astaxanthin via adonixanthin. Based on the bifunctional CRTO, a predicted pathway for astaxanthin biosynthesis in C. zofingiensis is described, and the CRTO is termed as carotenoid 4,4′-β-ionone ring oxygenase. © Springer-Verlag 2005.en_HK
dc.format.extent337398 bytes-
dc.format.extent3386 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.rightsThe original publication is available at www.springerlink.comen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshOxygenases - biosynthesis - genetics - isolation & purification - metabolismen_HK
dc.subject.meshChlorella - enzymology - geneticsen_HK
dc.subject.meshRandom Amplified Polymorphic DNA Techniqueen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA, Complementary - geneticsen_HK
dc.titleIsolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0175-7598&volume=71&issue=4&spage=473&epage=479&date=2006&atitle=Isolation+and+characterization+of+a+carotenoid+oxygenase+gene+from+Chlorella+zofingiensis+(Chlorophyta).en_HK
dc.identifier.emailHuang, JC: huangjc@hku.hken_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityHuang, JC=rp00710en_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1007/s00253-005-0166-8en_HK
dc.identifier.pmid16215714-
dc.identifier.scopuseid_2-s2.0-33745977148en_HK
dc.identifier.hkuros122401-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745977148&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume71en_HK
dc.identifier.issue4en_HK
dc.identifier.spage473en_HK
dc.identifier.epage479en_HK
dc.identifier.isiWOS:000239020100013-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridHuang, JC=7408108735en_HK
dc.identifier.scopusauthoridWang, Y=7601486161en_HK
dc.identifier.scopusauthoridSandmann, G=7006654333en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK

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