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Conference Paper: Identification and characterization of multiple osmotic response sequences in the human aldose reductase gene

TitleIdentification and characterization of multiple osmotic response sequences in the human aldose reductase gene
Authors
KeywordsBiology
BIOLOGY
Issue Date1997
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
The 17th International Congress of Biochemistry and Molecular Biology, San Francisco, California, USA, 24-29 August 1997, v. 11, p. 9 How to Cite?
AbstractAldose reductase (AR) has been implicated in osmoregulation in the kidney because it reduces glucose to sorbitol, which can serve as an osmolite. Under hyperosmotic stress, transcription of this gene is induced to increase the enzyme level. This mode of osmotic regulation of AR gene expression has been observed in a number of nonrenal cells as well, suggesting that this is a common response to hyperosmotic stress. We have identified a 132-base pair sequence 1 kilobase pairs upstream of the transcription start site of the human AR gene that enhances the transcription activity of the AR promoter as well as that of the SV40 promoter when the cells are under hyperosmotic stress. Within this 132-base pair sequence, there are three sequences that resemble TonE, the tonicity response element of the canine betaine transporter gene, and the osmotic response element of the rabbit AR gene, suggesting that the mechanism of osmotic regulation of gene expression in these animals is similar. These three sequences are designated as OreA, OreB, and OreC respectively, Analysis of the mouse AR gene also revealed that these three sequences are highly conserved between the mouse and human. Results from site-directed mutagenesis and gel mobility shift assays suggested that the OreC is the most important element for the osmotic response and cooperative interaction among the three elements in the human AR gene is essential for their enhancer function. The human aldose reductase gene osmotic response elements are the first osmotic response elements characterized in human.
Persistent Identifierhttp://hdl.handle.net/10722/47015
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorKo, BCBen_HK
dc.contributor.authorChung, SSMen_HK
dc.date.accessioned2007-10-30T07:04:10Z-
dc.date.available2007-10-30T07:04:10Z-
dc.date.issued1997en_HK
dc.identifier.citationThe 17th International Congress of Biochemistry and Molecular Biology, San Francisco, California, USA, 24-29 August 1997, v. 11, p. 9en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/47015-
dc.description.abstractAldose reductase (AR) has been implicated in osmoregulation in the kidney because it reduces glucose to sorbitol, which can serve as an osmolite. Under hyperosmotic stress, transcription of this gene is induced to increase the enzyme level. This mode of osmotic regulation of AR gene expression has been observed in a number of nonrenal cells as well, suggesting that this is a common response to hyperosmotic stress. We have identified a 132-base pair sequence 1 kilobase pairs upstream of the transcription start site of the human AR gene that enhances the transcription activity of the AR promoter as well as that of the SV40 promoter when the cells are under hyperosmotic stress. Within this 132-base pair sequence, there are three sequences that resemble TonE, the tonicity response element of the canine betaine transporter gene, and the osmotic response element of the rabbit AR gene, suggesting that the mechanism of osmotic regulation of gene expression in these animals is similar. These three sequences are designated as OreA, OreB, and OreC respectively, Analysis of the mouse AR gene also revealed that these three sequences are highly conserved between the mouse and human. Results from site-directed mutagenesis and gel mobility shift assays suggested that the OreC is the most important element for the osmotic response and cooperative interaction among the three elements in the human AR gene is essential for their enhancer function. The human aldose reductase gene osmotic response elements are the first osmotic response elements characterized in human.en_HK
dc.format.extent403448 bytes-
dc.format.extent1822 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_HK
dc.relation.ispartofFASEB Journalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBiologyen_HK
dc.subjectBIOLOGYen_HK
dc.titleIdentification and characterization of multiple osmotic response sequences in the human aldose reductase geneen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=11&spage=9&epage=&date=1997&atitle=Identification+and+characterization+of+multiple+osmotic+response+sequences+in+the+human+aldose+reductase+geneen_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.scopuseid_2-s2.0-33750179789en_HK
dc.identifier.hkuros36104-
dc.identifier.volume11en_HK
dc.identifier.issue9en_HK
dc.identifier.spageA1073en_HK
dc.identifier.epageA1073en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKo, BCB=7102833927en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK

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