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Conference Paper: Cell number quantification of USPIO-labeled stem cells by MRI: An in vitro study

TitleCell number quantification of USPIO-labeled stem cells by MRI: An in vitro study
Authors
KeywordsMedical sciences
Computer applications
Issue Date2006
PublisherIEEE.
Citation
Annual International Conference Of The Ieee Engineering In Medicine And Biology - Proceedings, 2006, p. 476-479 How to Cite?
AbstractMRI plays an expanding role in stem cell therapies. The non-invasive nature and high spatial resolution of MR imaging make MR imaging a powerful tool to investigate biologic processes at the molecular and cellular level in vivo longitudinally. Quantitative detection of stem cells after transplantation may allow assessment of stem cell localization and migration, and monitoring of the therapeutic effectiveness of stem cell therapy. In this study, we present a technique for MR quantification of magnetically labeled mouse embryonic stem cells distributed or injected in agarose gel phantoms. Apparent transverse relaxation rate enhancements (ΔR2*) were measured by gradient echo sequences. The linear relationship between ΔR2* and the concentration of USPIO-labeled mouse embryonic stem cells was observed and used for quantifying cell density and cell number after injection or transplantation. The MRI acquisition and analysis protocol were validated by good agreement between actual cell numbers and MRI-estimated cell numbers over a wide range of cell numbers. This MR technique for cell number and cell density quantification is applicable to future in vivo studies. © 2006 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/45727
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, JSen_HK
dc.contributor.authorChow, AMen_HK
dc.contributor.authorHui, ESen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2007-10-30T06:33:50Z-
dc.date.available2007-10-30T06:33:50Z-
dc.date.issued2006en_HK
dc.identifier.citationAnnual International Conference Of The Ieee Engineering In Medicine And Biology - Proceedings, 2006, p. 476-479en_HK
dc.identifier.issn0589-1019en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45727-
dc.description.abstractMRI plays an expanding role in stem cell therapies. The non-invasive nature and high spatial resolution of MR imaging make MR imaging a powerful tool to investigate biologic processes at the molecular and cellular level in vivo longitudinally. Quantitative detection of stem cells after transplantation may allow assessment of stem cell localization and migration, and monitoring of the therapeutic effectiveness of stem cell therapy. In this study, we present a technique for MR quantification of magnetically labeled mouse embryonic stem cells distributed or injected in agarose gel phantoms. Apparent transverse relaxation rate enhancements (ΔR2*) were measured by gradient echo sequences. The linear relationship between ΔR2* and the concentration of USPIO-labeled mouse embryonic stem cells was observed and used for quantifying cell density and cell number after injection or transplantation. The MRI acquisition and analysis protocol were validated by good agreement between actual cell numbers and MRI-estimated cell numbers over a wide range of cell numbers. This MR technique for cell number and cell density quantification is applicable to future in vivo studies. © 2006 IEEE.en_HK
dc.format.extent275942 bytes-
dc.format.extent2851 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherIEEE.en_HK
dc.relation.ispartofAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedingsen_HK
dc.rights©2006 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMedical sciencesen_HK
dc.subjectComputer applicationsen_HK
dc.subject.meshAnimals-
dc.subject.meshCell Count - Methods-
dc.subject.meshCell Line-
dc.subject.meshCells, Cultured-
dc.subject.meshContrast Media-
dc.subject.meshDextrans-
dc.subject.meshEmbryonic Stem Cells - Cytology-
dc.subject.meshFerumoxytol-
dc.subject.meshImage Enhancement - Methods-
dc.subject.meshImage Interpretation, Computer-Assisted - Methods-
dc.subject.meshIron - Diagnostic Use-
dc.subject.meshMagnetic Resonance Imaging - Methods-
dc.subject.meshMagnetite Nanoparticles-
dc.subject.meshMice-
dc.subject.meshOxides - Diagnostic Use-
dc.subject.meshStaining And Labeling-
dc.titleCell number quantification of USPIO-labeled stem cells by MRI: An in vitro studyen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1557-170X&volume=&spage=476&epage=479&date=2006&atitle=Cell+Number+Quantification+of+USPIO-labeled+Stem+Cells+by+MRI:+An+In+Vitro+Studyen_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1109/IEMBS.2006.259696en_HK
dc.identifier.pmid17945980-
dc.identifier.scopuseid_2-s2.0-84903806430en_HK
dc.identifier.hkuros132165-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34047124880&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.spage476en_HK
dc.identifier.epage479en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, JS=16174280400en_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridHui, ES=16175117100en_HK
dc.identifier.scopusauthoridYang, J=10041733800en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.citeulike3488626-

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