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Article: DNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonids

TitleDNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonids
Authors
KeywordsFish and fisheries
Issue Date1994
PublisherNational Research Council of Canada. The Journal's web site is located at http://www.uoguelph.ca/~cjfas
Citation
Canadian Journal of Fisheries and Aquatic Sciences, 1994, v. 51 n. Supp 1, p. 258-266 How to Cite?
AbstractWe used heterologous jeffreys' 33.6 core sequence and microsatellites (CAC)(5) and (CA)(12) as probes and compared them with probes based on the minisatellite sequences from tilapia (Oreochromis niloticus) and Atlantic salmon (Salmo salar) in fingerprinting assays. DNA fingerprints generated with the Jeffreys' 33.6 core sequence and the microsatellite (CAC)(5) and (CA)(12) probes showed complex profiles with high background, but DNA fingerprints using the tilapia and Atlantic salmon probes showed clear, less complex, informative, individual-specific DNA fingerprints suitable for analysis. We cloned and sequenced homologous repetitive sequences using a novel approach of creating a chinook salmon (Oncorhynchus tshawytscha) genomic DNA library with enriched low C(0)t DNA repeats for the development of DNA probes. The four types of repeats identified and sequenced were (CT)(n) and three Alu-like sequences. We generated DNA fingerprints using one of the minisatellite sequences as a probe. This minisatellite sequence was shown to be species specific because it is abundant in chinook and coho salmon (Oncorhynchus kisutch) genomes, but not in Atlantic salmon. These probes will provide us with the tools to study pedigree and linkage analysis, paternity testing, breeding programs, and the analysis of genetic structure within populations for aquaculture and fisheries research.
Persistent Identifierhttp://hdl.handle.net/10722/45442
ISSN
2015 Impact Factor: 2.437
2015 SCImago Journal Rankings: 1.271

 

DC FieldValueLanguage
dc.contributor.authorLeung, FCCen_HK
dc.contributor.authorWelt, Men_HK
dc.contributor.authorQuesenberry, RDen_HK
dc.contributor.authorShen, XZen_HK
dc.date.accessioned2007-10-30T06:25:40Z-
dc.date.available2007-10-30T06:25:40Z-
dc.date.issued1994en_HK
dc.identifier.citationCanadian Journal of Fisheries and Aquatic Sciences, 1994, v. 51 n. Supp 1, p. 258-266en_HK
dc.identifier.issn0706-652Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/45442-
dc.description.abstractWe used heterologous jeffreys' 33.6 core sequence and microsatellites (CAC)(5) and (CA)(12) as probes and compared them with probes based on the minisatellite sequences from tilapia (Oreochromis niloticus) and Atlantic salmon (Salmo salar) in fingerprinting assays. DNA fingerprints generated with the Jeffreys' 33.6 core sequence and the microsatellite (CAC)(5) and (CA)(12) probes showed complex profiles with high background, but DNA fingerprints using the tilapia and Atlantic salmon probes showed clear, less complex, informative, individual-specific DNA fingerprints suitable for analysis. We cloned and sequenced homologous repetitive sequences using a novel approach of creating a chinook salmon (Oncorhynchus tshawytscha) genomic DNA library with enriched low C(0)t DNA repeats for the development of DNA probes. The four types of repeats identified and sequenced were (CT)(n) and three Alu-like sequences. We generated DNA fingerprints using one of the minisatellite sequences as a probe. This minisatellite sequence was shown to be species specific because it is abundant in chinook and coho salmon (Oncorhynchus kisutch) genomes, but not in Atlantic salmon. These probes will provide us with the tools to study pedigree and linkage analysis, paternity testing, breeding programs, and the analysis of genetic structure within populations for aquaculture and fisheries research.en_HK
dc.format.extent2302259 bytes-
dc.format.extent572338 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.languageengen_HK
dc.publisherNational Research Council of Canada. The Journal's web site is located at http://www.uoguelph.ca/~cjfasen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectFish and fisheriesen_HK
dc.titleDNA fingerprinting and cloning of hypervariable minisatellite repeats in salmonidsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0706-652X&volume=51&issue=Supp 1&spage=258&epage=266&date=1994&atitle=DNA+fingerprinting+and+cloning+of+hypervariable+minisatellite+repeats+in+salmonidsen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.hkuros9683-

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