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Article: DNA-binding activity in the excretory-secretory products of Trichinella pseudospiralis (Nematoda: Trichinelloidea)

TitleDNA-binding activity in the excretory-secretory products of Trichinella pseudospiralis (Nematoda: Trichinelloidea)
Authors
Issue Date2001
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PAR
Citation
Parasitology (Cambridge), 2001, v. 123 n. pt 3, p. 301-308 How to Cite?
AbstractA novel DNA-binding peptide of Mr approximately 30 kDa was documented for the first time in the excretory-secretory (E-S) products of the infective-stage larvae of Trichinella pseudospiralis. Larvae recovered from muscles of infected mice were maintained for 48 h in DMEM medium. E-S products of worms extracted from the medium were analysed for DNA-binding activity by the electrophoretic mobility shift assay (EMSA). Multiple DNA-protein complexes were detected. A comparison of the Mr of proteins in the complexes indicated that they could bind to the target DNA as a dimer, tetramer or multiples of tetramers. Site selection and competition analysis showed that the binding has a low specificity. A (G/C-rich)-gap-(G/T-rich)-DNA sequence pattern was extracted from a pool of degenerate PCR fragments binding to the E-S products. Results of immunoprecipitation and electrophoretic mobility supershift assay confirmed the authenticity of the DNA-binding protein as an E-S product.
Persistent Identifierhttp://hdl.handle.net/10722/45440
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.327
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, CHen_HK
dc.contributor.authorKo, RCCen_HK
dc.date.accessioned2007-10-30T06:25:37Z-
dc.date.available2007-10-30T06:25:37Z-
dc.date.issued2001en_HK
dc.identifier.citationParasitology (Cambridge), 2001, v. 123 n. pt 3, p. 301-308en_HK
dc.identifier.issn0031-1820en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45440-
dc.description.abstractA novel DNA-binding peptide of Mr approximately 30 kDa was documented for the first time in the excretory-secretory (E-S) products of the infective-stage larvae of Trichinella pseudospiralis. Larvae recovered from muscles of infected mice were maintained for 48 h in DMEM medium. E-S products of worms extracted from the medium were analysed for DNA-binding activity by the electrophoretic mobility shift assay (EMSA). Multiple DNA-protein complexes were detected. A comparison of the Mr of proteins in the complexes indicated that they could bind to the target DNA as a dimer, tetramer or multiples of tetramers. Site selection and competition analysis showed that the binding has a low specificity. A (G/C-rich)-gap-(G/T-rich)-DNA sequence pattern was extracted from a pool of degenerate PCR fragments binding to the E-S products. Results of immunoprecipitation and electrophoretic mobility supershift assay confirmed the authenticity of the DNA-binding protein as an E-S product.en_HK
dc.format.extent548305 bytes-
dc.format.extent3069 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PARen_HK
dc.rightsParasitology (Cambridge). Copyright © Cambridge University Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshDNA-Binding Proteins - chemistry - physiology - secretionen_HK
dc.subject.meshHelminth Proteins - chemistry - physiology - secretionen_HK
dc.subject.meshTrichinella - chemistry - metabolism - physiologyen_HK
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_HK
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_HK
dc.titleDNA-binding activity in the excretory-secretory products of Trichinella pseudospiralis (Nematoda: Trichinelloidea)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-1820&volume=123&issue=pt 3&spage=301&epage=308&date=2001&atitle=DNA-binding+activity+in+the+excretory-secretory+products+of+Trichinella+pseudospiralis+(Nematoda:+Trichinelloidea)en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0031182001008459en_HK
dc.identifier.pmid11578094-
dc.identifier.scopuseid_2-s2.0-0034834899-
dc.identifier.hkuros66192-
dc.identifier.isiWOS:000171485400011-

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