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Article: Identification of some heat-induced genes of Trichinella spiralis

TitleIdentification of some heat-induced genes of Trichinella spiralis
Authors
Issue Date2001
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PAR
Citation
Parasitology (Cambridge), 2001, v. 123 n. pt 3, p. 293-300 How to Cite?
AbstractThree heat-induced genes of the infective-stage larvae of Trichinella spiralis were successfully identified by the suppression subtractive hybridization (SSH) technique. As indicated by reverse Northern blotting, 19 of 25 clones were scored as differentially transcribed in the heat-shocked infective-stage larvae. The sequencing data showed the presence of 12 different genes. Three were homologous to histone H3, histone H2B and translationally controlled tumour protein (TCTP). A 0.6 kb cDNA of histone H3 was generated by the RACE method and sequenced. It contained an open reading frame of 136 amino acids that demonstrated 94% identity with genes from Drosophila hydei. Semi-quantitative RT-PCR indicated that after heat-shock treatment, the expression levels of histone H3, histone H2B and TCTP increased 4.8, 27 and 5.7-fold, respectively. Northern analysis confirmed the upregulation of histone H3, histone H2B and TCTP transcripts. The upregulation of these genes during stress conditions has not been reported in parasitic organisms. The stress proteins may play an active role to sustain the parasite after exposure to hostile host factors.
Persistent Identifierhttp://hdl.handle.net/10722/45439
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.327
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, ASCen_HK
dc.contributor.authorSun, KWen_HK
dc.contributor.authorKo, RCCen_HK
dc.date.accessioned2007-10-30T06:25:35Z-
dc.date.available2007-10-30T06:25:35Z-
dc.date.issued2001en_HK
dc.identifier.citationParasitology (Cambridge), 2001, v. 123 n. pt 3, p. 293-300en_HK
dc.identifier.issn0031-1820en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45439-
dc.description.abstractThree heat-induced genes of the infective-stage larvae of Trichinella spiralis were successfully identified by the suppression subtractive hybridization (SSH) technique. As indicated by reverse Northern blotting, 19 of 25 clones were scored as differentially transcribed in the heat-shocked infective-stage larvae. The sequencing data showed the presence of 12 different genes. Three were homologous to histone H3, histone H2B and translationally controlled tumour protein (TCTP). A 0.6 kb cDNA of histone H3 was generated by the RACE method and sequenced. It contained an open reading frame of 136 amino acids that demonstrated 94% identity with genes from Drosophila hydei. Semi-quantitative RT-PCR indicated that after heat-shock treatment, the expression levels of histone H3, histone H2B and TCTP increased 4.8, 27 and 5.7-fold, respectively. Northern analysis confirmed the upregulation of histone H3, histone H2B and TCTP transcripts. The upregulation of these genes during stress conditions has not been reported in parasitic organisms. The stress proteins may play an active role to sustain the parasite after exposure to hostile host factors.en_HK
dc.format.extent355996 bytes-
dc.format.extent1771 bytes-
dc.format.extent3069 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PARen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsParasitology (Cambridge). Copyright © Cambridge University Press.en_HK
dc.subject.meshCalcium-Binding Proteins - biosynthesis - chemistry - geneticsen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshHeat-Shock Proteins - biosynthesis - chemistry - geneticsen_HK
dc.subject.meshHistones - biosynthesis - chemistry - geneticsen_HK
dc.subject.meshTrichinella spiralis - chemistry - genetics - physiologyen_HK
dc.titleIdentification of some heat-induced genes of Trichinella spiralisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-1820&volume=123&issue=pt 3&spage=293&epage=300&date=2001&atitle=Identification+of+some+heat-induced+genes+of+Trichinella+spiralisen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0031182001008320en_HK
dc.identifier.pmid11578093-
dc.identifier.scopuseid_2-s2.0-0034832813-
dc.identifier.hkuros66191-
dc.identifier.isiWOS:000171485400010-
dc.identifier.citeulike6821250-

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