File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Pituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish

TitlePituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish
Authors
KeywordsAnd teleost
Calcium
cAMP
Goldfish
Gonadotropin-II
Growth hormone
PAC1 receptor
PACAP
Pituitary cells
Protein kinase A
Protein kinase C
VIP
VPAC1 receptor
VPAC2 receptor
Issue Date2000
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?bcb
Citation
Biochemistry And Cell Biology, 2000, v. 78 n. 3, p. 329-343 How to Cite?
AbstractPituitary adenylate cyclase activating polypeptide (PACAP) is a novel member of the secretin-glucagon peptide family. In mammals, this peptide has been located in a wide range of tissues and is involved in a variety of biological functions. In lower vertebrates, especially fish, increasing evidence suggests that PACAP may function as a hypophysiotropic factor regulating pituitary hormone secretion. PACAP has been identified in the brain-pituitary axis of representative fish species. The molecular structure of fish PACAP is highly homologous to mammalian PACAP. The prepro-PACAP in fish, however, is distinct from that of mammals as it also contains the sequence of fish GHRH. In teleosts, the anterior pituitary is under direct innervation of the hypothalamus and PACAP nerve fibers have been identified in the pars distalis. Using the goldfish as a fish model, mRNA transcripts of PACAP receptors, namely the PAC1 and VPAC1 receptors, have been identified in the pituitary as well as in various brain areas. Consistent with the pituitary expression of PACAP receptors, PACAP analogs are effective in stimulating growth hormone (GH) and gonadotropin (GTH)-II secretion in the goldfish both in vivo and in vitro. The GH-releasing action of PACAP is mediated via pituitary PAC1 receptors coupled to the adenylate cyclase-cAMP-protein kinase A and phospholipase C-IP3-protein kinase C pathways. Subsequent stimulation of Ca 2+ entry through voltage-sensitive Ca 2+ channels followed by activation of Ca 2+-calmodulin protein kinase II is likely the downstream mechanism mediating PACAP-stimulated GH release in goldfish. Although the PACAP receptor subtype(s) and the associated post-receptor signaling events responsible for PACAP-stimulated GTH-II release have not been characterized in goldfish, these findings support the hypothesis that PACAP is produced in the hypothalamus and delivered to the anterior pituitary to regulate GH and GTH-II release in fish.
Persistent Identifierhttp://hdl.handle.net/10722/45438
ISSN
2015 Impact Factor: 1.527
2015 SCImago Journal Rankings: 0.859
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorLi, WSen_HK
dc.contributor.authorLee, EKYen_HK
dc.contributor.authorLeung, MYen_HK
dc.contributor.authorTse, LYen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorLin, HRen_HK
dc.contributor.authorChang, JPen_HK
dc.date.accessioned2007-10-30T06:25:33Z-
dc.date.available2007-10-30T06:25:33Z-
dc.date.issued2000en_HK
dc.identifier.citationBiochemistry And Cell Biology, 2000, v. 78 n. 3, p. 329-343en_HK
dc.identifier.issn0829-8211en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45438-
dc.description.abstractPituitary adenylate cyclase activating polypeptide (PACAP) is a novel member of the secretin-glucagon peptide family. In mammals, this peptide has been located in a wide range of tissues and is involved in a variety of biological functions. In lower vertebrates, especially fish, increasing evidence suggests that PACAP may function as a hypophysiotropic factor regulating pituitary hormone secretion. PACAP has been identified in the brain-pituitary axis of representative fish species. The molecular structure of fish PACAP is highly homologous to mammalian PACAP. The prepro-PACAP in fish, however, is distinct from that of mammals as it also contains the sequence of fish GHRH. In teleosts, the anterior pituitary is under direct innervation of the hypothalamus and PACAP nerve fibers have been identified in the pars distalis. Using the goldfish as a fish model, mRNA transcripts of PACAP receptors, namely the PAC1 and VPAC1 receptors, have been identified in the pituitary as well as in various brain areas. Consistent with the pituitary expression of PACAP receptors, PACAP analogs are effective in stimulating growth hormone (GH) and gonadotropin (GTH)-II secretion in the goldfish both in vivo and in vitro. The GH-releasing action of PACAP is mediated via pituitary PAC1 receptors coupled to the adenylate cyclase-cAMP-protein kinase A and phospholipase C-IP3-protein kinase C pathways. Subsequent stimulation of Ca 2+ entry through voltage-sensitive Ca 2+ channels followed by activation of Ca 2+-calmodulin protein kinase II is likely the downstream mechanism mediating PACAP-stimulated GH release in goldfish. Although the PACAP receptor subtype(s) and the associated post-receptor signaling events responsible for PACAP-stimulated GTH-II release have not been characterized in goldfish, these findings support the hypothesis that PACAP is produced in the hypothalamus and delivered to the anterior pituitary to regulate GH and GTH-II release in fish.en_HK
dc.format.extent1677524 bytes-
dc.format.extent2366 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?bcben_HK
dc.relation.ispartofBiochemistry and Cell Biologyen_HK
dc.rightsBiochemistry and Cell Biology. Copyright © N R C Research Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAnd teleosten_HK
dc.subjectCalciumen_HK
dc.subjectcAMPen_HK
dc.subjectGoldfishen_HK
dc.subjectGonadotropin-IIen_HK
dc.subjectGrowth hormoneen_HK
dc.subjectPAC1 receptoren_HK
dc.subjectPACAPen_HK
dc.subjectPituitary cellsen_HK
dc.subjectProtein kinase Aen_HK
dc.subjectProtein kinase Cen_HK
dc.subjectVIPen_HK
dc.subjectVPAC1 receptoren_HK
dc.subjectVPAC2 receptoren_HK
dc.subject.meshNeuropeptides - metabolism - pharmacology - physiologyen_HK
dc.subject.meshCalcium Channels - metabolismen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshPituitary Adenylate Cyclase-Activating Polypeptideen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.titlePituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fishen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0829-8211&volume=78&issue=3&spage=329&epage=343&date=2000&atitle=Pituitary+adenylate+cyclase+activating+polypeptide+as+a+novel+hypophysiotropic+factor+in+fishen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.emailLeung, MY: kmyleung@hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.identifier.authorityLeung, MY=rp00733en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10949084-
dc.identifier.scopuseid_2-s2.0-0033851205en_HK
dc.identifier.hkuros53073-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033851205&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume78en_HK
dc.identifier.issue3en_HK
dc.identifier.spage329en_HK
dc.identifier.epage343en_HK
dc.identifier.isiWOS:000088408600016-
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridLi, WS=36062301700en_HK
dc.identifier.scopusauthoridLee, EKY=7406968652en_HK
dc.identifier.scopusauthoridLeung, MY=7401860738en_HK
dc.identifier.scopusauthoridTse, LY=36891045500en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridLin, HR=7405570040en_HK
dc.identifier.scopusauthoridChang, JP=7601547649en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats