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Article: Single-stranded endonuclease activity in the excretory--secretory products of Trichinella spiralis and Trichinella pseudospiralis

TitleSingle-stranded endonuclease activity in the excretory--secretory products of Trichinella spiralis and Trichinella pseudospiralis
Authors
Issue Date2000
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PAR
Citation
Parasitology (Cambridge), 2000, v. 120 n. pt 5, p. 527-533 How to Cite?
AbstractA novel acidic extracellular single-stranded endonuclease was demonstrated for the first time in the excretory-secretory (E-S) products of 2 species of Trichinella. Unlike the double-stranded endonuclease reported earlier, the single-stranded molecule is divalent cation independent and is detected in both T. spiralis and T. pseudospiralis E-S products. It hydrolysed single-stranded DNA and RNA at comparable rates. The single-stranded endonuclease was sensitive to inhibition by Zn2+ and to high concentrations of NaCl. Zymographic analysis indicated that it was encoded by at least 3 peptides of Mr approximately 50-60 kDa. The rate of hydrolysis of single-stranded targets by the E-S products was substantially higher than that of the double-stranded molecule. Due to the differences in peptide profile, divalent cation dependence, and species-specific expression, the single and double-stranded endonucleases are likely to be encoded by different proteins and may have different functions.
Persistent Identifierhttp://hdl.handle.net/10722/45437
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.327
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMak, Cen_HK
dc.contributor.authorChung, YYYen_HK
dc.contributor.authorKo, RCCen_HK
dc.date.accessioned2007-10-30T06:25:32Z-
dc.date.available2007-10-30T06:25:32Z-
dc.date.issued2000en_HK
dc.identifier.citationParasitology (Cambridge), 2000, v. 120 n. pt 5, p. 527-533en_HK
dc.identifier.issn0031-1820en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45437-
dc.description.abstractA novel acidic extracellular single-stranded endonuclease was demonstrated for the first time in the excretory-secretory (E-S) products of 2 species of Trichinella. Unlike the double-stranded endonuclease reported earlier, the single-stranded molecule is divalent cation independent and is detected in both T. spiralis and T. pseudospiralis E-S products. It hydrolysed single-stranded DNA and RNA at comparable rates. The single-stranded endonuclease was sensitive to inhibition by Zn2+ and to high concentrations of NaCl. Zymographic analysis indicated that it was encoded by at least 3 peptides of Mr approximately 50-60 kDa. The rate of hydrolysis of single-stranded targets by the E-S products was substantially higher than that of the double-stranded molecule. Due to the differences in peptide profile, divalent cation dependence, and species-specific expression, the single and double-stranded endonucleases are likely to be encoded by different proteins and may have different functions.en_HK
dc.format.extent220614 bytes-
dc.format.extent1833 bytes-
dc.format.extent3069 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PARen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsParasitology (Cambridge). Copyright © Cambridge University Press.en_HK
dc.subject.meshEndonucleases - antagonists & inhibitors - metabolismen_HK
dc.subject.meshHelminth Proteins - metabolismen_HK
dc.subject.meshTrichinella - enzymologyen_HK
dc.subject.meshTrichinella spiralis - enzymologyen_HK
dc.subject.meshTrichinosis - parasitologyen_HK
dc.titleSingle-stranded endonuclease activity in the excretory--secretory products of Trichinella spiralis and Trichinella pseudospiralisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-1820&volume=120&issue=pt 5&spage=527&epage=533&date=2000&atitle=Single-stranded+endonuclease+activity+in+the+excretory--secretory+products+of+Trichinella+spiralis+and+Trichinella+pseudospiralisen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0031182099005879en_HK
dc.identifier.pmid10840982en_HK
dc.identifier.scopuseid_2-s2.0-0034019506-
dc.identifier.hkuros52911-
dc.identifier.isiWOS:000087528500010-

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