File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Heat shock response of Trichinella spiralis and T. pseudospiralis

TitleHeat shock response of Trichinella spiralis and T. pseudospiralis
Authors
Issue Date1996
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PAR
Citation
Parasitology (Cambridge), 1996, v. 112 n. pt 1, p. 89-95 How to Cite?
AbstractHeat shock proteins (HSPs) were documented for the first time in both somatic extracts and excretory/secretory (ES) products of the infective-stage larvae of Trichinella spiralis and T. pseudospiralis. Larvae recovered from muscles of infected mice were heat shocked at 37, 40, 43 and 45 degrees C in RPMI 1640 medium containing L(-)[35S]methionine. Somatic extracts and ES products of heat-shocked worms were then analysed by SDS-PAGE, autoradiography and laser densitometry. Prominent bands of HSPs were observed at 43 degrees C which is the optimal heat shock temperature. The major HSPs in somatic extracts of T. spiralis were 20, 47, 50, 70, 80 and 86 kDa. When the temperature was increased from 37 to 43 degrees C, the greatest increase in absorbance was observed in HSPs 70 and 86. In vitro translation of mRNA in a nuclease-treated rabbit reticulocyte lysate system showed an increase in the synthesis of the 80 kDa protein. This suggests that the production of HSP 80 is regulated at the transcriptional level. The major HSPs in the ES products were 11, 45, 53 and 64 kDa. In T. pseudospiralis, the major HSPs in the somatic extracts were 20, 26, 31, 50, 53, 70, 80 and 86 kDa, and in the ES products, 11, 35, 37, 41 and 64 kDa.
Persistent Identifierhttp://hdl.handle.net/10722/45436
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.327
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKo, RCCen_HK
dc.contributor.authorFan, Len_HK
dc.date.accessioned2007-10-30T06:25:31Z-
dc.date.available2007-10-30T06:25:31Z-
dc.date.issued1996en_HK
dc.identifier.citationParasitology (Cambridge), 1996, v. 112 n. pt 1, p. 89-95en_HK
dc.identifier.issn0031-1820en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45436-
dc.description.abstractHeat shock proteins (HSPs) were documented for the first time in both somatic extracts and excretory/secretory (ES) products of the infective-stage larvae of Trichinella spiralis and T. pseudospiralis. Larvae recovered from muscles of infected mice were heat shocked at 37, 40, 43 and 45 degrees C in RPMI 1640 medium containing L(-)[35S]methionine. Somatic extracts and ES products of heat-shocked worms were then analysed by SDS-PAGE, autoradiography and laser densitometry. Prominent bands of HSPs were observed at 43 degrees C which is the optimal heat shock temperature. The major HSPs in somatic extracts of T. spiralis were 20, 47, 50, 70, 80 and 86 kDa. When the temperature was increased from 37 to 43 degrees C, the greatest increase in absorbance was observed in HSPs 70 and 86. In vitro translation of mRNA in a nuclease-treated rabbit reticulocyte lysate system showed an increase in the synthesis of the 80 kDa protein. This suggests that the production of HSP 80 is regulated at the transcriptional level. The major HSPs in the ES products were 11, 45, 53 and 64 kDa. In T. pseudospiralis, the major HSPs in the somatic extracts were 20, 26, 31, 50, 53, 70, 80 and 86 kDa, and in the ES products, 11, 35, 37, 41 and 64 kDa.en_HK
dc.format.extent3247815 bytes-
dc.format.extent3069 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=PARen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsParasitology (Cambridge). Copyright © Cambridge University Press.en_HK
dc.subject.meshHeat-Shock Proteins - biosynthesis - geneticsen_HK
dc.subject.meshHelminth Proteins - biosynthesis - geneticsen_HK
dc.subject.meshTrichinella - genetics - metabolismen_HK
dc.subject.meshTrichinella spiralis - genetics - metabolismen_HK
dc.subject.meshProtein Biosynthesisen_HK
dc.titleHeat shock response of Trichinella spiralis and T. pseudospiralisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0031-1820&volume=112&issue=pt 1&spage=89&epage=95&date=1996&atitle=Heat+shock+response+of+Trichinella+spiralis+and+T.+pseudospiralisen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8587806-
dc.identifier.scopuseid_2-s2.0-0030065985-
dc.identifier.hkuros20896-
dc.identifier.isiWOS:A1996TP76700010-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats