File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Dopaminergic and GABAergic amacrine cells are direct targets of melatonin: Immunocytochemical study of mt1 melatonin receptor in guinea pig retina

TitleDopaminergic and GABAergic amacrine cells are direct targets of melatonin: Immunocytochemical study of mt1 melatonin receptor in guinea pig retina
Authors
KeywordsMt1 Melatonin receptor
Immunocytochemistry
Tyrosine hydroxylase
GABA
Retina
Issue Date2000
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNS
Citation
Visual Neuroscience, 2000, v. 17 n. 1, p. 63-70 How to Cite?
AbstractDistribution of the mt1 melatonin receptor in the guinea pig retina was immunocytochemically investigated using peptide-specific anti-mt1 receptor antibody. Western blots of the guinea pig retina showed a single band at approximately 37 kilodalton (kD) immunoreactive to the anti-mt1 antibody. The most intense immunoreactivity for the mt1 receptor was detected in the cell bodies of ganglion cells. Their dendrites and axons were also immunolabeled. Subpopulations of amacrine cells, the inner plexiform layer, and the outer plexiform layer also exhibited moderate to weak immunolabeling. The mt1-positive amacrine cells were located either at the vitreal border of the inner nuclear layer or displaced in the ganglion cell layer. Double immunolabeling using antibodies to the mt1 receptor and tyrosine hydroxylase revealed that the majority of dopaminergic amacrine cells showed mt1 immunoreactivity. Almost all the 1CA type dopaminergic cells were mt1 positive while the 2CA type cells less frequently exhibited mt1 immunoreaction. By double immunolabeling for the mt1 receptor and GABA, more than 50% of the mt1-immunoreactive amacrine cells were shown to be GABAergic neurons. Approximately one-third of the GABAergic amacrine cells were immunolabeled for the mt1 receptor. The present results demonstrate expression of the mt1 receptor in diverse neuronal cell types in the guinea pig retina and provide the first evidence for the direct effect of melatonin on dopaminergic and GABAergic amacrine cells via the mt1 receptor.
Persistent Identifierhttp://hdl.handle.net/10722/45288
ISSN
2015 Impact Factor: 1.871
2015 SCImago Journal Rankings: 1.231
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFujieda, Hen_HK
dc.contributor.authorScher, Jen_HK
dc.contributor.authorHamadanizadeh, SAen_HK
dc.contributor.authorWankiewicz, Een_HK
dc.contributor.authorPang, SFen_HK
dc.contributor.authorBrown, GMen_HK
dc.date.accessioned2007-10-30T06:21:52Z-
dc.date.available2007-10-30T06:21:52Z-
dc.date.issued2000en_HK
dc.identifier.citationVisual Neuroscience, 2000, v. 17 n. 1, p. 63-70en_HK
dc.identifier.issn0952-5238en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45288-
dc.description.abstractDistribution of the mt1 melatonin receptor in the guinea pig retina was immunocytochemically investigated using peptide-specific anti-mt1 receptor antibody. Western blots of the guinea pig retina showed a single band at approximately 37 kilodalton (kD) immunoreactive to the anti-mt1 antibody. The most intense immunoreactivity for the mt1 receptor was detected in the cell bodies of ganglion cells. Their dendrites and axons were also immunolabeled. Subpopulations of amacrine cells, the inner plexiform layer, and the outer plexiform layer also exhibited moderate to weak immunolabeling. The mt1-positive amacrine cells were located either at the vitreal border of the inner nuclear layer or displaced in the ganglion cell layer. Double immunolabeling using antibodies to the mt1 receptor and tyrosine hydroxylase revealed that the majority of dopaminergic amacrine cells showed mt1 immunoreactivity. Almost all the 1CA type dopaminergic cells were mt1 positive while the 2CA type cells less frequently exhibited mt1 immunoreaction. By double immunolabeling for the mt1 receptor and GABA, more than 50% of the mt1-immunoreactive amacrine cells were shown to be GABAergic neurons. Approximately one-third of the GABAergic amacrine cells were immunolabeled for the mt1 receptor. The present results demonstrate expression of the mt1 receptor in diverse neuronal cell types in the guinea pig retina and provide the first evidence for the direct effect of melatonin on dopaminergic and GABAergic amacrine cells via the mt1 receptor.en_HK
dc.format.extent410190 bytes-
dc.format.extent1843 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=VNSen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsVisual Neuroscience. Copyright © Cambridge University Press.en_HK
dc.subjectMt1 Melatonin receptoren_HK
dc.subjectImmunocytochemistryen_HK
dc.subjectTyrosine hydroxylaseen_HK
dc.subjectGABAen_HK
dc.subjectRetinaen_HK
dc.titleDopaminergic and GABAergic amacrine cells are direct targets of melatonin: Immunocytochemical study of mt1 melatonin receptor in guinea pig retinaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-5238&volume=17&issue=1&spage=63&epage=70&date=2000&atitle=Dopaminergic+and+GABAergic+amacrine+cells+are+direct+targets+of+melatonin:+Immunocytochemical+study+of+mt1+melatonin+receptor+in+guinea+pig+retinaen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0952523800171068en_HK
dc.identifier.pmid10750827-
dc.identifier.scopuseid_2-s2.0-0034022819-
dc.identifier.isiWOS:000085927000006-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats