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Article: Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis

TitleUse of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis
Authors
Issue Date1993
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 1993, v. 46 n. 4, p. 318-322 How to Cite?
AbstractAims - To assess the routine use of a polymerase chain reaction (PCR) assay for the direct detection of Mycobacterium tuberculosis in expectorated sputum specimens. Methods - A pair of primers (20-mer) were designed to amplify the 38 kilodalton protein of M tuberculosis. The specificity of the assay was evaluated in 31 M tuberculosis strains, 15 atypical mycobacterium species, and several commensal bacteria of the upper respiratory tract. The assay was subsequently applied to 519 sputum specimens from 85 inpatients of a chest hospital in Hong Kong. Results - An amplified product of 239 base pairs was found in all M tuberculosis strains, standard strains of M bovis, and M africanum but not in the other bacterial strains tested. For the 51 patients with pulmonary radiographic lesions, the diagnosis of pulmonary tuberculosis was subsequently confirmed by both culture and PCR in 41 of them. Five patients who were treated before admission were positive by PCR alone. All but one patient in the control group (patients with acute exacerbation of chronic obstructive airway diseases) or those with atypical mycobacterial diseases were PCR negative. The PCR remained positive after four weeks of anti-tuberculosis treatment in 29 patients, 16 of whom had become culture negative. Conclusion - This PCR assay is a useful technique for the diagnosis of untreated and recently treated cases of pulmonary tuberculosis.
Persistent Identifierhttp://hdl.handle.net/10722/45180
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChan, KSen_HK
dc.contributor.authorChan, CMen_HK
dc.contributor.authorHo, BSWen_HK
dc.contributor.authorDai, LKen_HK
dc.contributor.authorChau, PYen_HK
dc.contributor.authorNg, MHen_HK
dc.date.accessioned2007-10-30T06:19:11Z-
dc.date.available2007-10-30T06:19:11Z-
dc.date.issued1993en_HK
dc.identifier.citationJournal Of Clinical Pathology, 1993, v. 46 n. 4, p. 318-322en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45180-
dc.description.abstractAims - To assess the routine use of a polymerase chain reaction (PCR) assay for the direct detection of Mycobacterium tuberculosis in expectorated sputum specimens. Methods - A pair of primers (20-mer) were designed to amplify the 38 kilodalton protein of M tuberculosis. The specificity of the assay was evaluated in 31 M tuberculosis strains, 15 atypical mycobacterium species, and several commensal bacteria of the upper respiratory tract. The assay was subsequently applied to 519 sputum specimens from 85 inpatients of a chest hospital in Hong Kong. Results - An amplified product of 239 base pairs was found in all M tuberculosis strains, standard strains of M bovis, and M africanum but not in the other bacterial strains tested. For the 51 patients with pulmonary radiographic lesions, the diagnosis of pulmonary tuberculosis was subsequently confirmed by both culture and PCR in 41 of them. Five patients who were treated before admission were positive by PCR alone. All but one patient in the control group (patients with acute exacerbation of chronic obstructive airway diseases) or those with atypical mycobacterial diseases were PCR negative. The PCR remained positive after four weeks of anti-tuberculosis treatment in 29 patients, 16 of whom had become culture negative. Conclusion - This PCR assay is a useful technique for the diagnosis of untreated and recently treated cases of pulmonary tuberculosis.en_HK
dc.format.extent981804 bytes-
dc.format.extent1783 bytes-
dc.format.extent2150 bytes-
dc.format.extent7189 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshTuberculosis,-Pulmonary-diagnosisen_HK
dc.titleUse of PCR in routine diagnosis of treated and untreated pulmonary tuberculosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=46&issue=4&spage=318&epage=322&date=1993&atitle=Use+of+PCR+in+routine+diagnosis+of+treated+and+untreated+pulmonary+tuberculosisen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8496388-
dc.identifier.pmcidPMC501211-
dc.identifier.scopuseid_2-s2.0-0027318136en_HK
dc.identifier.volume46en_HK
dc.identifier.issue4en_HK
dc.identifier.spage318en_HK
dc.identifier.epage322en_HK
dc.identifier.isiWOS:A1993KW66600008-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridChan, KS=36448818400en_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridHo, BSW=36640210100en_HK
dc.identifier.scopusauthoridDai, LK=36874953100en_HK
dc.identifier.scopusauthoridChau, PY=29267442500en_HK
dc.identifier.scopusauthoridNg, MH=7202076421en_HK

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