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Article: Early diagnosis of primary human herpesvirus 6 infection in childhood: Serology, polymerase chain reaction, and virus load

TitleEarly diagnosis of primary human herpesvirus 6 infection in childhood: Serology, polymerase chain reaction, and virus load
Authors
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org
Citation
Journal Of Infectious Diseases, 1998, v. 178 n. 5, p. 1250-1256 How to Cite?
AbstractQualitative and quantitative polymerase chain reaction (PCR) for human herpesvirus 6 (HHV-6) DNA in whole blood and plasma was correlated with serology and clinical assessment in 143 children hospitalized for undifferentiated febrile illness to evaluate options for diagnosis of primary HHV-6 infection on the acute blood specimen. PCR and serology for HHV-7 were done in parallel to define serologic cross-reactions. Using HHV-6 seroconversion as the reference standard, detection of HHV-6 DNA in whole blood in the absence of antibody in the plasma was the most reliable evidence of primary HHV-6 infection. Detection of HHV-6 DNA in plasma and a high virus load in whole blood (>3.3 log 10 copies/5 μL) had a sensitivity of 90% and 100%, respectively, in diagnosing primary HHV-6 infection. However, both were occasionally found in patients with other infections, possibly associated with HHV-6 reactivation. Maternal antibody may confound interpretation of serology in patients under 3 months of age.
Persistent Identifierhttp://hdl.handle.net/10722/45178
ISSN
2015 Impact Factor: 6.344
2015 SCImago Journal Rankings: 4.000

 

DC FieldValueLanguage
dc.contributor.authorChiu, SSen_HK
dc.contributor.authorCheung, CYen_HK
dc.contributor.authorTse, CYCen_HK
dc.contributor.authorPeiris, Men_HK
dc.date.accessioned2007-10-30T06:19:08Z-
dc.date.available2007-10-30T06:19:08Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Infectious Diseases, 1998, v. 178 n. 5, p. 1250-1256en_HK
dc.identifier.issn0022-1899en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45178-
dc.description.abstractQualitative and quantitative polymerase chain reaction (PCR) for human herpesvirus 6 (HHV-6) DNA in whole blood and plasma was correlated with serology and clinical assessment in 143 children hospitalized for undifferentiated febrile illness to evaluate options for diagnosis of primary HHV-6 infection on the acute blood specimen. PCR and serology for HHV-7 were done in parallel to define serologic cross-reactions. Using HHV-6 seroconversion as the reference standard, detection of HHV-6 DNA in whole blood in the absence of antibody in the plasma was the most reliable evidence of primary HHV-6 infection. Detection of HHV-6 DNA in plasma and a high virus load in whole blood (>3.3 log 10 copies/5 μL) had a sensitivity of 90% and 100%, respectively, in diagnosing primary HHV-6 infection. However, both were occasionally found in patients with other infections, possibly associated with HHV-6 reactivation. Maternal antibody may confound interpretation of serology in patients under 3 months of age.en_HK
dc.format.extent258994 bytes-
dc.format.extent2677 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org en_HK
dc.relation.ispartofJournal of Infectious Diseasesen_HK
dc.rightsJournal of Infectious Diseases. Copyright © University of Chicago Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshExanthema-Subitum-diagnosisen_HK
dc.subject.meshViral-Loaden_HK
dc.titleEarly diagnosis of primary human herpesvirus 6 infection in childhood: Serology, polymerase chain reaction, and virus loaden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1899&volume=178&issue=5&spage=1250&epage=1256&date=1998&atitle=Early+diagnosis+of+primary+human+herpesvirus+6+infection+in+childhood:+serology,+polymerase+chain+reaction,+and+virus+loaden_HK
dc.identifier.emailChiu, SS: ssschiu@hku.hken_HK
dc.identifier.emailPeiris, M: malik@hkucc.hku.hken_HK
dc.identifier.authorityChiu, SS=rp00421en_HK
dc.identifier.authorityPeiris, M=rp00410en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1086/314432en_HK
dc.identifier.pmid9780243-
dc.identifier.scopuseid_2-s2.0-0031755699en_HK
dc.identifier.hkuros44816-
dc.identifier.volume178en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1250en_HK
dc.identifier.epage1256en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, SS=7202291500en_HK
dc.identifier.scopusauthoridCheung, CY=55437572500en_HK
dc.identifier.scopusauthoridTse, CYC=36958767800en_HK
dc.identifier.scopusauthoridPeiris, M=7005486823en_HK

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