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Article: A latex slide agglutination test for rapid detection of antimyeloperoxidase antibody

TitleA latex slide agglutination test for rapid detection of antimyeloperoxidase antibody
Authors
Issue Date1999
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal of Clinical Pathology, 1999, v. 52 n. 10, p. 770-772 How to Cite?
AbstractAIM: To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum. METHODS: Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin. RESULTS: There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO. CONCLUSIONS: MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.
Persistent Identifierhttp://hdl.handle.net/10722/45177
ISSN
2015 Impact Factor: 2.912
2015 SCImago Journal Rankings: 1.260
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKo, KHen_HK
dc.contributor.authorLee, SSen_HK
dc.contributor.authorLawton, JWen_HK
dc.date.accessioned2007-10-30T06:19:06Z-
dc.date.available2007-10-30T06:19:06Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal of Clinical Pathology, 1999, v. 52 n. 10, p. 770-772en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45177-
dc.description.abstractAIM: To develop and test a new latex slide agglutination test (MPO-LSAT) to detect antimyeloperoxidase (anti-MPO) antibody in serum. METHODS: Latex bead coating was adjusted to give maximum sensitivity by attending to latex size, MPO to latex ratio for coupling, ratio of diluted serum to MPO-latex, reaction time and temperature for coupling, and reaction time for agglutination. Inhibition studies were performed using MPO, proteinase 3, bactericidal/permeability increasing protein, and lactoferrin. RESULTS: There was very good correlation between this test and the conventional anti-MPO enzyme linked immunosorbent assay (ELISA): 81% of sera positive in the ELISA were positive by MPO-LSAT. MPO-LSAT results correlated better with IgM anti-MPO than with IgG anti-MPO. CONCLUSIONS: MPO-LSAT is a simple diagnostic test that is potentially useful in the clinical laboratory as a rapid screening tool for vasculitic diseases.en_HK
dc.format.extent105542 bytes-
dc.format.extent2424 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.subject.meshAutoantibodies-blooden_HK
dc.subject.meshPeroxidase-immunologyen_HK
dc.subject.meshVasculitis-diagnosisen_HK
dc.titleA latex slide agglutination test for rapid detection of antimyeloperoxidase antibodyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=52&issue=10&spage=770&epage=772&date=1999&atitle=A+latex+slide+agglutination+test+for+rapid+detection+of+antimyeloperoxidase+antibodyen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10674037-
dc.identifier.pmcidPMC501574-
dc.identifier.scopuseid_2-s2.0-0032888556-
dc.identifier.isiWOS:000082996700014-

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