Article: Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia
| Title | Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia |
|---|---|
| Authors | Woo, PCY2 Lau, SKP2 Tsoi, HW2 Huang, Y2 Poon, RWS2 Chu, CM4 Lee, RA3 Luk, WK1 Wong, GKM2 Wong, BHL2 Cheng, VCC2 Tang, BSF2 Wu, AKL2 Yung, RWH3 Chen, H2 Guan, Y2 Chan, KH2 Yuen, KY2 |
| Issue Date | 2005 |
| Publisher | Oxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org |
| Citation | Journal Of Infectious Diseases, 2005, v. 192 n. 11, p. 1898-1907 [How to Cite?] DOI: http://dx.doi.org/10.1086/497151 |
| Abstract | Background. Recently, we described the discovery of a novel group 2 coronavirus, coronavirus HKU1 (CoV-HKU1), from a patient with pneumonia. However, the clinical and molecular epidemiological features of CoV-HKU1-associated pneumonia are unknown. Methods. Prospectively collected (during a 12-month period) nasopharyngeal aspirates (NPAs) from patients with community-acquired pneumonia from 4 hospitals were subjected to reverse-transcription polymerase chain reaction, for detection of CoV-HKU1. The epidemiological, clinical, and laboratory characteristics of patients with CoV-HKU1-associated pneumonia were analyzed. The pol, spike (S), and nucleocapsid (N) genes were also sequenced. Results. NPAs from 10 (2.4%) of 418 patients with community-acquired pneumonia were found to be positive for CoV-HKU1. All 10 cases occurred in spring and winter. Nine of these patients were adults, and 4 had underlying diseases of the respiratory tract. In the 6 patients from whom serum samples were available, all had a 4-fold change in immunoglobulin (Ig) G titer and/or presence of IgM against CoV-HKU1. The 2 patients who died had significantly lower hemoglobin levels, monocyte counts, albumin levels, and oxygen saturation levels on admission and had more-extensive involvement visible on chest radiographs. Sequence analysis of the pol, S, and N genes revealed 2 genotypes of CoV-HKU1. Conclusions. CoV-HKU1 accounts for 2.4% of community-acquired pneumonia, with 2 genotypes in the study population. Without performance of diagnostic tests, the illness was clinically indistinguishable from other community-acquired pneumonia illnesses. © 2005 by the Infectious Diseases Society of America. All rights reserved. |
| ISSN | 0022-1899 2011 Impact Factor: 6.41 2011 SCImago Journal Rankings: 0.831 |
| DOI | http://dx.doi.org/10.1086/497151 |
| ISI Accession Number ID | WOS:000233018200007 |
| References | References in Scopus |
| dc.contributor.author | Woo, PCY |
|---|---|
| dc.contributor.author | Lau, SKP |
| dc.contributor.author | Tsoi, HW |
| dc.contributor.author | Huang, Y |
| dc.contributor.author | Poon, RWS |
| dc.contributor.author | Chu, CM |
| dc.contributor.author | Lee, RA |
| dc.contributor.author | Luk, WK |
| dc.contributor.author | Wong, GKM |
| dc.contributor.author | Wong, BHL |
| dc.contributor.author | Cheng, VCC |
| dc.contributor.author | Tang, BSF |
| dc.contributor.author | Wu, AKL |
| dc.contributor.author | Yung, RWH |
| dc.contributor.author | Chen, H |
| dc.contributor.author | Guan, Y |
| dc.contributor.author | Chan, KH |
| dc.contributor.author | Yuen, KY |
| dc.date.accessioned | 2007-10-30T06:19:01Z |
| dc.date.available | 2007-10-30T06:19:01Z |
| dc.date.issued | 2005 |
| dc.description.abstract | Background. Recently, we described the discovery of a novel group 2 coronavirus, coronavirus HKU1 (CoV-HKU1), from a patient with pneumonia. However, the clinical and molecular epidemiological features of CoV-HKU1-associated pneumonia are unknown. Methods. Prospectively collected (during a 12-month period) nasopharyngeal aspirates (NPAs) from patients with community-acquired pneumonia from 4 hospitals were subjected to reverse-transcription polymerase chain reaction, for detection of CoV-HKU1. The epidemiological, clinical, and laboratory characteristics of patients with CoV-HKU1-associated pneumonia were analyzed. The pol, spike (S), and nucleocapsid (N) genes were also sequenced. Results. NPAs from 10 (2.4%) of 418 patients with community-acquired pneumonia were found to be positive for CoV-HKU1. All 10 cases occurred in spring and winter. Nine of these patients were adults, and 4 had underlying diseases of the respiratory tract. In the 6 patients from whom serum samples were available, all had a 4-fold change in immunoglobulin (Ig) G titer and/or presence of IgM against CoV-HKU1. The 2 patients who died had significantly lower hemoglobin levels, monocyte counts, albumin levels, and oxygen saturation levels on admission and had more-extensive involvement visible on chest radiographs. Sequence analysis of the pol, S, and N genes revealed 2 genotypes of CoV-HKU1. Conclusions. CoV-HKU1 accounts for 2.4% of community-acquired pneumonia, with 2 genotypes in the study population. Without performance of diagnostic tests, the illness was clinically indistinguishable from other community-acquired pneumonia illnesses. © 2005 by the Infectious Diseases Society of America. All rights reserved. |
| dc.description.nature | published_or_final_version |
| dc.format.extent | 650187 bytes |
| dc.format.extent | 1831689 bytes |
| dc.format.extent | 2713 bytes |
| dc.format.mimetype | application/pdf |
| dc.format.mimetype | application/pdf |
| dc.format.mimetype | text/plain |
| dc.identifier.citation | Journal Of Infectious Diseases, 2005, v. 192 n. 11, p. 1898-1907 [How to Cite?] DOI: http://dx.doi.org/10.1086/497151 |
| dc.identifier.doi | http://dx.doi.org/10.1086/497151 |
| dc.identifier.epage | 1907 |
| dc.identifier.hkuros | 114686 |
| dc.identifier.isi | WOS:000233018200007 |
| dc.identifier.issn | 0022-1899 2011 Impact Factor: 6.41 2011 SCImago Journal Rankings: 0.831 |
| dc.identifier.issue | 11 |
| dc.identifier.openurl | ![]() |
| dc.identifier.pmid | 16267760 |
| dc.identifier.scopus | eid_2-s2.0-27544516178 |
| dc.identifier.spage | 1898 |
| dc.identifier.uri | http://hdl.handle.net/10722/45173 |
| dc.identifier.volume | 192 |
| dc.language | eng |
| dc.publisher | Oxford University Press. The Journal's web site is located at http://jid.oxfordjournals.org |
| dc.publisher.place | United States |
| dc.relation.ispartof | Journal of Infectious Diseases |
| dc.relation.references | References in Scopus |
| dc.rights | Journal of Infectious Diseases. Copyright © University of Chicago Press. |
| dc.rights | Creative Commons: Attribution 3.0 Hong Kong License |
| dc.subject.mesh | Community-Acquired-Infections-epidemiology |
| dc.subject.mesh | Community-Acquired-Infections-mortality |
| dc.subject.mesh | Community-Acquired-Infections-physiopathology |
| dc.subject.mesh | Community-Acquired-Infections-virology |
| dc.subject.mesh | Coronavirus-genetics |
| dc.title | Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia |
| dc.type | Article |
Author Affiliations
- Tseung Kwan O Hospital
- The University of Hong Kong
- Pamela Youde Nethersole Eastern Hospital
- United Christian Hospital Hong Kong


