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Article: Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures

TitleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
Authors
Issue Date2006
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
Citation
Journal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222 How to Cite?
Abstract
Using full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
Persistent Identifierhttp://hdl.handle.net/10722/45165
ISSN
2013 Impact Factor: 2.551
PubMed Central ID
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital
DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorNg, KHLen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorYip, KTen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorWoo, GKSen_HK
dc.contributor.authorChan, KMen_HK
dc.contributor.authorQue, TIen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2007-10-30T06:18:48Z-
dc.date.available2007-10-30T06:18:48Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222en_HK
dc.identifier.issn0021-9746en_HK
dc.identifier.urihttp://hdl.handle.net/10722/45165-
dc.description.abstractUsing full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.en_HK
dc.format.extent145305 bytes-
dc.format.extent1831689 bytes-
dc.format.extent7189 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacteria,-Anaerobic-classificationen_HK
dc.subject.meshBacterial-Typing-Techniques-methodsen_HK
dc.subject.meshDNA,-Bacterial-geneticsen_HK
dc.subject.meshGram-Positive-Bacteria-classificationen_HK
dc.subject.meshGram-Positive-Bacterial-Infections-diagnosisen_HK
dc.titleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood culturesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=59&issue=2&spage=219&epage=222&date=2006&atitle=Usefulness+of+the+MicroSeq+500+16S+rDNA+bacterial+identification+system+for+identification+of+anaerobic+Gram+positive+bacilli+isolated+from+blood+culturesen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1136/jcp.2004.025247en_HK
dc.identifier.pmid16443743en_HK
dc.identifier.pmcidPMC1860316-
dc.identifier.scopuseid_2-s2.0-32544458383en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32544458383&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue2en_HK
dc.identifier.spage219en_HK
dc.identifier.epage222en_HK
dc.identifier.isiWOS:000234938100020-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridNg, KHL=27467782600en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridYip, KT=7101909925en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridWoo, GKS=7006485416en_HK
dc.identifier.scopusauthoridChan, KM=7406034671en_HK
dc.identifier.scopusauthoridQue, TI=7003786628en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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