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Article: Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
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TitleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
 
AuthorsLau, SKP1
Ng, KHL2
Woo, PCY1
Yip, KT2
Fung, AMY1
Woo, GKS1
Chan, KM1
Que, TI2
Yuen, KY1
 
Issue Date2006
 
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
CitationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2004.025247
 
AbstractUsing full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
 
ISSN0021-9746
2012 Impact Factor: 2.439
2012 SCImago Journal Rankings: 0.980
 
DOIhttp://dx.doi.org/10.1136/jcp.2004.025247
 
PubMed Central IDPMC1860316
 
ISI Accession Number IDWOS:000234938100020
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLau, SKP
 
dc.contributor.authorNg, KHL
 
dc.contributor.authorWoo, PCY
 
dc.contributor.authorYip, KT
 
dc.contributor.authorFung, AMY
 
dc.contributor.authorWoo, GKS
 
dc.contributor.authorChan, KM
 
dc.contributor.authorQue, TI
 
dc.contributor.authorYuen, KY
 
dc.date.accessioned2007-10-30T06:18:48Z
 
dc.date.available2007-10-30T06:18:48Z
 
dc.date.issued2006
 
dc.description.abstractUsing full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
 
dc.description.naturepublished_or_final_version
 
dc.format.extent145305 bytes
 
dc.format.extent1831689 bytes
 
dc.format.extent7189 bytes
 
dc.format.mimetypeapplication/pdf
 
dc.format.mimetypeapplication/pdf
 
dc.format.mimetypetext/plain
 
dc.identifier.citationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2004.025247
 
dc.identifier.doihttp://dx.doi.org/10.1136/jcp.2004.025247
 
dc.identifier.epage222
 
dc.identifier.isiWOS:000234938100020
 
dc.identifier.issn0021-9746
2012 Impact Factor: 2.439
2012 SCImago Journal Rankings: 0.980
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC1860316
 
dc.identifier.pmid16443743
 
dc.identifier.scopuseid_2-s2.0-32544458383
 
dc.identifier.spage219
 
dc.identifier.urihttp://hdl.handle.net/10722/45165
 
dc.identifier.volume59
 
dc.languageeng
 
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Clinical Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshBacteria,-Anaerobic-classification
 
dc.subject.meshBacterial-Typing-Techniques-methods
 
dc.subject.meshDNA,-Bacterial-genetics
 
dc.subject.meshGram-Positive-Bacteria-classification
 
dc.subject.meshGram-Positive-Bacterial-Infections-diagnosis
 
dc.titleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Tuen Mun Hospital