Article: Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures

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TitleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
AuthorsLau, SKP2
Ng, KHL1
Woo, PCY2
Yip, KT1
Fung, AMY2
Woo, GKS2
Chan, KM2
Que, TI1
Yuen, KY2
Issue Date2006
PublisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
CitationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2004.025247
AbstractUsing full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
ISSN0021-9746
2011 Impact Factor: 2.306
2011 SCImago Journal Rankings: 0.236
DOIhttp://dx.doi.org/10.1136/jcp.2004.025247
PubMed Central IDPMC1860316
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLau, SKP
dc.contributor.authorNg, KHL
dc.contributor.authorWoo, PCY
dc.contributor.authorYip, KT
dc.contributor.authorFung, AMY
dc.contributor.authorWoo, GKS
dc.contributor.authorChan, KM
dc.contributor.authorQue, TI
dc.contributor.authorYuen, KY
dc.date.accessioned2007-10-30T06:18:48Z
dc.date.available2007-10-30T06:18:48Z
dc.date.issued2006
dc.description.abstractUsing full 16S ribosomal RNA (rRNA) gene sequencing as the gold standard, 20 non-duplicating anaerobic Gram positive bacilli isolated from blood cultures were analysed by the MicroSeq 500 16S rDNA bacterial identification system. The MicroSeq system successfully identified 13 of the 20 isolates. Four and three isolates were misidentified at the genus and species level, respectively. Although the MicroSeq 500 16S rDNA bacterial identification system is better than three commercially available identification systems also evaluated, its database needs to be expanded for accurate identification of anaerobic Gram positive bacilli.
dc.description.naturepublished_or_final_version
dc.format.extent145305 bytes
dc.format.extent1831689 bytes
dc.format.extent7189 bytes
dc.format.mimetypeapplication/pdf
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.identifier.citationJournal Of Clinical Pathology, 2006, v. 59 n. 2, p. 219-222 [How to Cite?]
DOI: http://dx.doi.org/10.1136/jcp.2004.025247
dc.identifier.doihttp://dx.doi.org/10.1136/jcp.2004.025247
dc.identifier.epage222
dc.identifier.isiWOS:000234938100020
dc.identifier.issn0021-9746
2011 Impact Factor: 2.306
2011 SCImago Journal Rankings: 0.236
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmcidPMC1860316
dc.identifier.pmid16443743
dc.identifier.scopuseid_2-s2.0-32544458383
dc.identifier.spage219
dc.identifier.urihttp://hdl.handle.net/10722/45165
dc.identifier.volume59
dc.languageeng
dc.publisherB M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofJournal of Clinical Pathology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Clinical Pathology. Copyright © B M J Publishing Group.
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshBacteria,-Anaerobic-classification
dc.subject.meshBacterial-Typing-Techniques-methods
dc.subject.meshDNA,-Bacterial-genetics
dc.subject.meshGram-Positive-Bacteria-classification
dc.subject.meshGram-Positive-Bacterial-Infections-diagnosis
dc.titleUsefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures
dc.typeArticle
Author Affiliations
  1. Tuen Mun Hospital
  2. The University of Hong Kong