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Article: Expression of the mouse α1(II) collagen gene is not restricted to cartilage during development

TitleExpression of the mouse α1(II) collagen gene is not restricted to cartilage during development
Authors
Issue Date1991
PublisherCompany of Biologists Ltd.
Citation
Development, 1991, v. 111 n. 4, p. 945-935 How to Cite?
AbstractThe mouse alpha 1(II) collagen gene has been isolated and a 5' portion of the gene which has low homology to other collagen genes was used to study the pattern of expression during mouse embryogenesis. In situ hybridization studies show that in the mouse, like the chick, alpha 1(II) collagen is expressed in chondrogenic tissues in advance of chondrocyte differentiation. The gene is expressed early in embryogenesis at 9.5 days both in the cranial mesenchyme destined for the chondrocranium, and the sclerotome of the somites, and at 12.5 days in the primordia of the hyoid and the laryngeal cartilage. Type II collagen gene transcripts were found in all the chondrogenic tissues of the axial and appendicular skeleton until the onset of endochondral ossification. Expression of alpha 1(II) collagen mRNA was also observed in non-chondrogenic tissues such as the notochord which may be responsible for inducing chondrogenesis in somitic mesoderm, neural retina, the corneal and conjunctival epithelia and sclera of the developing eye. Expression in the tail tendon was late, at 16.5-18.5 days. Transient expression was also found in the heart at 9.5-12.5 days, the epidermis at 10.5-14.5 days, the calvarial mesenchyme at 12.5-16.5 days, the inner ear at 14.5 days and the fetal brain from 9.5-14.5 days. Within the neural tube, alpha 1(II) collagen mRNA was localized in the proliferative ventricular cells of the forebrain and midbrain of 9.5- to 10.5-day embryos. Subsequently, transcription of the alpha 1(II) collagen gene was confined to restricted areas of the rhombencephalic basal plate, the ventricular layer of the hindbrain and the cervical spinal cord. These examples of expression of the type II collagen gene in the developing nervous system seem to suggest that active transcription of this gene might be associated with early stages of neuroblast differentiation. Type II collagen may therefore have additional roles in development unrelated to chondrogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/44579
ISSN
2015 Impact Factor: 6.059
2015 SCImago Journal Rankings: 5.239
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorLau, ETen_HK
dc.contributor.authorAu, PKCen_HK
dc.contributor.authorTam, PPLen_HK
dc.date.accessioned2007-10-30T06:04:54Z-
dc.date.available2007-10-30T06:04:54Z-
dc.date.issued1991en_HK
dc.identifier.citationDevelopment, 1991, v. 111 n. 4, p. 945-935en_HK
dc.identifier.issn0950-1991en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44579-
dc.description.abstractThe mouse alpha 1(II) collagen gene has been isolated and a 5' portion of the gene which has low homology to other collagen genes was used to study the pattern of expression during mouse embryogenesis. In situ hybridization studies show that in the mouse, like the chick, alpha 1(II) collagen is expressed in chondrogenic tissues in advance of chondrocyte differentiation. The gene is expressed early in embryogenesis at 9.5 days both in the cranial mesenchyme destined for the chondrocranium, and the sclerotome of the somites, and at 12.5 days in the primordia of the hyoid and the laryngeal cartilage. Type II collagen gene transcripts were found in all the chondrogenic tissues of the axial and appendicular skeleton until the onset of endochondral ossification. Expression of alpha 1(II) collagen mRNA was also observed in non-chondrogenic tissues such as the notochord which may be responsible for inducing chondrogenesis in somitic mesoderm, neural retina, the corneal and conjunctival epithelia and sclera of the developing eye. Expression in the tail tendon was late, at 16.5-18.5 days. Transient expression was also found in the heart at 9.5-12.5 days, the epidermis at 10.5-14.5 days, the calvarial mesenchyme at 12.5-16.5 days, the inner ear at 14.5 days and the fetal brain from 9.5-14.5 days. Within the neural tube, alpha 1(II) collagen mRNA was localized in the proliferative ventricular cells of the forebrain and midbrain of 9.5- to 10.5-day embryos. Subsequently, transcription of the alpha 1(II) collagen gene was confined to restricted areas of the rhombencephalic basal plate, the ventricular layer of the hindbrain and the cervical spinal cord. These examples of expression of the type II collagen gene in the developing nervous system seem to suggest that active transcription of this gene might be associated with early stages of neuroblast differentiation. Type II collagen may therefore have additional roles in development unrelated to chondrogenesis.en_HK
dc.format.extent1568475 bytes-
dc.format.extent2034 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherCompany of Biologists Ltd.en_HK
dc.relation.ispartofDevelopmenten_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCartilage-embryologyen_HK
dc.subject.meshCollagen-geneticsen_HK
dc.subject.meshGene-Expression-physiologyen_HK
dc.subject.meshMesoderm-physiologyen_HK
dc.subject.meshNervous-System-embryologyen_HK
dc.titleExpression of the mouse α1(II) collagen gene is not restricted to cartilage during developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-1991&volume=111&issue=4&spage=945&epage=953&date=1991&atitle=Expression+of+the+mouse+alpha+1(II)+collagen+gene+is+not+restricted+to+cartilage+during+developmenten_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid1879363-
dc.identifier.scopuseid_2-s2.0-0025732094en_HK
dc.identifier.volume111en_HK
dc.identifier.issue4en_HK
dc.identifier.spage945en_HK
dc.identifier.epage935en_HK
dc.identifier.isiWOS:A1991FK57600010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridLau, ET=7103086081en_HK
dc.identifier.scopusauthoridAu, PKC=36789243400en_HK
dc.identifier.scopusauthoridTam, PPL=7202539412en_HK

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