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Article: Human COL2A1-directed SV40 T antigen expression in transgenic and chimeric mice results in abnormal skeletal development

TitleHuman COL2A1-directed SV40 T antigen expression in transgenic and chimeric mice results in abnormal skeletal development
Authors
Issue Date1995
PublisherRockefeller University Press. The Journal's web site is located at http://www.jcb.org
Citation
Journal Of Cell Biology, 1995, v. 128 n. 1-2, p. 223-237 How to Cite?
AbstractThe ability of SV40 T antigen to cause abnormalities in cartilage development in transgenic mice and chimeras has been tested. The cis- regulatory elements of the COL2A1 gene were used to target expression of SV40 T antigen to differentiating chondrocytes in transgenic mice and chimeras derived from embryonal stem (ES) cells bearing the same transgene. The major phenotypic consequences of transgenic (pAL21) expression are malformed skeleton, disproportionate dwarfism, and perinatal/neonatal death. Expression of T antigen was tissue specific and in the main characteristic of the mouse α1(II) collagen gene. Chondrocyte densities and levels of α1(II) collagen mRNAs were reduced in the transgenic mice. Islands of cells which express cartilage characteristic genes such as type IIB procollagen, long form α1(IX) collagen, α2(XI) collagen, and aggrecan were found in the articular and growth cartilages of pAL21 chimeric fetuses and neonates. But these cells, which were expressing T antigen, were not properly organized into columns of proliferating chondrocytes. Levels of α1(II) collagen mRNA were reduced in these chondrocytes. In addition, these cells did not express type X collagen, a marker for hypertrophic chondrocytes. The skeletal abnormality in pAL21 mice may therefore be due to a retardation of chondrocyte maturation or an impaired ability of chondrocytes to complete terminal differentiation and an associated paucity of some cartilage matrix components.
Persistent Identifierhttp://hdl.handle.net/10722/44578
ISSN
2015 Impact Factor: 8.717
2015 SCImago Journal Rankings: 7.923
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorLevy, Aen_HK
dc.contributor.authorTrainor, PAen_HK
dc.contributor.authorWai, AWKen_HK
dc.contributor.authorKuffner, Ten_HK
dc.contributor.authorChi Leung Soen_HK
dc.contributor.authorLeung, KKHen_HK
dc.contributor.authorLovellBadge, RHen_HK
dc.contributor.authorTam, PPLen_HK
dc.date.accessioned2007-10-30T06:04:52Z-
dc.date.available2007-10-30T06:04:52Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Cell Biology, 1995, v. 128 n. 1-2, p. 223-237en_HK
dc.identifier.issn0021-9525en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44578-
dc.description.abstractThe ability of SV40 T antigen to cause abnormalities in cartilage development in transgenic mice and chimeras has been tested. The cis- regulatory elements of the COL2A1 gene were used to target expression of SV40 T antigen to differentiating chondrocytes in transgenic mice and chimeras derived from embryonal stem (ES) cells bearing the same transgene. The major phenotypic consequences of transgenic (pAL21) expression are malformed skeleton, disproportionate dwarfism, and perinatal/neonatal death. Expression of T antigen was tissue specific and in the main characteristic of the mouse α1(II) collagen gene. Chondrocyte densities and levels of α1(II) collagen mRNAs were reduced in the transgenic mice. Islands of cells which express cartilage characteristic genes such as type IIB procollagen, long form α1(IX) collagen, α2(XI) collagen, and aggrecan were found in the articular and growth cartilages of pAL21 chimeric fetuses and neonates. But these cells, which were expressing T antigen, were not properly organized into columns of proliferating chondrocytes. Levels of α1(II) collagen mRNA were reduced in these chondrocytes. In addition, these cells did not express type X collagen, a marker for hypertrophic chondrocytes. The skeletal abnormality in pAL21 mice may therefore be due to a retardation of chondrocyte maturation or an impaired ability of chondrocytes to complete terminal differentiation and an associated paucity of some cartilage matrix components.en_HK
dc.format.extent9383645 bytes-
dc.format.extent1831 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jcb.orgen_HK
dc.relation.ispartofJournal of Cell Biologyen_HK
dc.rightsThe Journal of Cell Biology. Copyright © Rockefeller University Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAbnormalities-geneticsen_HK
dc.subject.meshAntigens,-Polyomavirus-Transforming-biosynthesisen_HK
dc.subject.meshBone-and-Bones-abnormalitiesen_HK
dc.subject.meshChimera-en_HK
dc.subject.meshCollagen-geneticsen_HK
dc.titleHuman COL2A1-directed SV40 T antigen expression in transgenic and chimeric mice results in abnormal skeletal developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9525&volume=128&issue=1-2&spage=223&epage=237&date=1995&atitle=Human+COL2A1-directed+SV40+T+antigen+expression+in+transgenic+and+chimeric+mice+results+in+abnormal+skeletal+developmenten_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.emailLeung, KKH:keithlee@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.identifier.authorityLeung, KKH=rp00298en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid7822417-
dc.identifier.pmcidPMC2120328-
dc.identifier.scopuseid_2-s2.0-0028877808en_HK
dc.identifier.hkuros1709-
dc.identifier.volume128en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage223en_HK
dc.identifier.epage237en_HK
dc.identifier.isiWOS:A1995QB80600020-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridLevy, A=7401905663en_HK
dc.identifier.scopusauthoridTrainor, PA=7003894254en_HK
dc.identifier.scopusauthoridWai, AWK=6603455255en_HK
dc.identifier.scopusauthoridKuffner, T=6603038707en_HK
dc.identifier.scopusauthoridChi Leung So=7409943371en_HK
dc.identifier.scopusauthoridLeung, KKH=7401860467en_HK
dc.identifier.scopusauthoridLovellBadge, RH=7006432550en_HK
dc.identifier.scopusauthoridTam, PPL=7202539412en_HK

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